Recombination between immunoglobulin variable region gene segments is enhanced by transcription (original) (raw)
- Letter
- Published: 11 December 1986
- Mark W. Moore1 na1,
- George D. Yancopoulos1,
- Heikyung Suh1,
- Stuart Lutzker1,
- Erik Selsing1 na1 &
- …
- Frederick W. Alt1
Nature volume 324, pages 585–589 (1986)Cite this article
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Abstract
Immunoglobulin (Ig) variable (V) region genes are assembled in precursor B (pre-B) lymphocytes from multiple germline segments. The heavy-chain _V_- region gene is composed of variable (VH), diversity (D) and joining (_J_H) segments; kappa (κ) and lambda (λ) light-chain _V_-region genes have analogous _V_L, and _J_L segments. Assembly of Ig _V_-gene segments, as well as those of the highly related T-cell receptor, is regulated at several levels and shows both stage and tissue specificity; for example Ig heavy-chain _V_-gene assembly precedes that of Ig light chains during B-cell differentiation1. Joining of all classes of _V_-gene segments involves conserved recognition sequences that are probably targets for a common recombinase2. Evidence has been presented suggesting that rearrangement of specific classes of segments is regulated by modulation of their accessibility to the recombinase3. To elucidate mechanisms which control _V_-region gene assembly, we have investigated the effect of flanking gene expression on the frequency at which introduced _V_-gene segments are assembled in pre-B cell lines. Our findings suggest that transcription may play a direct role in the regulation of immunoglobulin _V_-gene assembly.
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Author notes
- Mark W. Moore and Erik Selsing: Rosensteil Basic Medical Sciences Research Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA
Authors and Affiliations
- Department of Biochemistry and Microbiology, Institute for Cancer Research, Columbia University College of Physicians and Surgeons, 630 W. 168th Street, New York, New York, 10032, USA
T. Keith Blackwell, Mark W. Moore, George D. Yancopoulos, Heikyung Suh, Stuart Lutzker, Erik Selsing & Frederick W. Alt
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- T. Keith Blackwell
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Blackwell, T., Moore, M., Yancopoulos, G. et al. Recombination between immunoglobulin variable region gene segments is enhanced by transcription.Nature 324, 585–589 (1986). https://doi.org/10.1038/324585a0
- Received: 26 June 1986
- Accepted: 23 September 1986
- Issue Date: 11 December 1986
- DOI: https://doi.org/10.1038/324585a0