Thy-1-mediated T-cell activation requires co-expression of CD3/Ti complex (original) (raw)

Nature volume 326, pages 505–507 (1987)Cite this article

Abstract

In addition to monoclonal antibodies against the CD3 (T3)-T-cell antigen receptor (CD3/Ti) complex1–3, several other monoclonals directed towards distinct cell surface structures on human (CD2 (T11)4 and Tp445,6) and murine (Thy-17–9, TAP10, and Ly-611) T lymphocytes are capable of activating T cells. It has been proposed that such structures may function as alternative pathways of stimulation. To examine directly whether any relationship exists between Thy-1-dependent activation phenomena and T-cell activation mediated through the CD3/Ti complex, we have transfected several CD3/Ti– variants12,13 of the human T-cell line Jurkat with the murine Thy-1.2 gene. Our data indicate that in CD3/Ti–, Thy-1.2+ transfectants, monoclonal antibodies against Thy-1.2 can induce a rise in cytoplasmic free calcium ([Ca2+]i), but fail to stimulate interleukin-2 (IL-2) production. The only defect in these variant cell lines responsible for the inability to produce IL-2 in response to Thy-1 stimulation was in the expression of the CD3/Ti complex, because replacement of defective Ti α- or β-chain genes reconstributed both surface expression of CD3/Ti and responsiveness to Thy-1 in the IL-2 production assay.

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References

  1. Chang, T. W., Kung, P. C., Gingras, S. P. & Goldstein, G. Proc. natn. Acad. Sci. U.S.A. 78, 1805–1808 (1982).
    Article ADS Google Scholar
  2. Reinherz, E. L. et al. Immun. Rev. 81, 95–129 (1984).
    Article CAS Google Scholar
  3. Haskins, K. et al. J. exp. Med. 157, 1149–1169 (1983).
    Article CAS Google Scholar
  4. Meuer, S. C. et al. Cell 36, 897–906 (1984).
    Article CAS Google Scholar
  5. Hara, T., Fu, S. M. & Hansen, J. A. J. exp. Med. 161, 1513–1524 (1985).
    Article CAS Google Scholar
  6. Moretta, A., Pantako, G., Lopez-Botet, M. & Moretta, L. J. exp. Med. 162, 823–838 (1985).
    Article CAS Google Scholar
  7. Gunter, K. C., Malek, T. R. & Shevach, E. M. J. exp. Med. 159, 716–730 (1984).
    Article CAS Google Scholar
  8. MacDonald, H. R., Bron, C., Rousseaux, M., Horvath, C. & Cerottini, J.-C. Eur. J. Immun. 15, 495–501 (1985).
    Article CAS Google Scholar
  9. Kroczek, R. A., Gunter, K. C., Seligmann, B. & Shevach, E. M. J. Immun. 136, 4379–4384 (1986).
    CAS PubMed Google Scholar
  10. Rock, K. L. et al. J. exp. Med. 163, 315–333 (1986).
    Article CAS Google Scholar
  11. Malek, T. R., Ortega, G., Chan, C., Kroczek, R. A. & Shevach, E. M. J. exp. Med. 164, 709–722 (1986).
    Article CAS Google Scholar
  12. Weiss, A. & Stobo, J. D. J. exp. Med. 160, 1284–1299 (1984).
    Article CAS Google Scholar
  13. Ohashi, P. S. et al. Nature 316, 606–609 (1985).
    Article ADS CAS Google Scholar
  14. Gunter, K. C., Kroczek, R. A., Shevach, E. M. & Germain, R. N. J. exp. Med. 163, 285–300 (1986).
    Article CAS Google Scholar
  15. Kroczek, R. A., Gunter, K. C., Germain, R. N. & Shevach, E. M. Nature 322, 181–183 (1986).
    Article ADS CAS Google Scholar
  16. Saito, T., Weiss, A., Miller, J., Norcross, M. & Germain, R. N. Nature 325, 125–130 (1987).
    Article ADS CAS Google Scholar
  17. Weiss, A., Manger, B. & Imboden, I. J. Immun. 137, 819–825 (1986).
    CAS PubMed Google Scholar
  18. O'Flynn, K. et al. Eur. J. Immun. 16, 580–584 (1986).
    Article CAS Google Scholar
  19. June, C. H. et al. J. clin. Invest. 77, 1224–1232 (1986).
    Article CAS Google Scholar
  20. Low, M. G. & Kincade, P. W. Nature 318, 62–64 (1985).
    Article ADS CAS Google Scholar

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Author notes

  1. Arthur Weiss: Howard Hughes Medical Institue and the Department of Medicine, University of California San Francisco, San Francisco, California 94143, USA

Authors and Affiliations

  1. Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20892, USA
    Kurt C. Gunter, Ronald N. Germain, Richard A. Kroczek, Takashi Saito, Wayne M. Yokoyama, Christina Chan, Arthur Weiss & Ethan M. Shevach

Authors

  1. Kurt C. Gunter
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  2. Ronald N. Germain
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  3. Richard A. Kroczek
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  4. Takashi Saito
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  5. Wayne M. Yokoyama
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  6. Christina Chan
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  7. Arthur Weiss
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  8. Ethan M. Shevach
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Gunter, K., Germain, R., Kroczek, R. et al. Thy-1-mediated T-cell activation requires co-expression of CD3/Ti complex.Nature 326, 505–507 (1987). https://doi.org/10.1038/326505a0

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