Two zinc fingers of a yeast regulatory protein shown by genetic evidence to be essential for its function (original) (raw)

Nature volume 328, pages 443–445 (1987) Cite this article

Abstract

The best-understood protein structure involved in DNA binding is the helix-turn-helix motif1. A second DNA-binding domain, the finger structure, has been proposed on the basis of sequence analysis2,3, partial proteolysis2 and zinc content2 of Xenopus transcription factor TFIIIA. Other eukaryotic proteins were subsequently found to contain contiguous repeat units of the postulated finger motif4–6. Each repeat unit contains thirty amino acids and is thought to bind a zinc atom using two cysteines and two histidines as ligands. The protein loop or finger between apparent zinc ligands is rich in DNA-binding residues and is thought to make specific contacts with DNA2. The yeast protein ADR1, a positive regulator of transcription of the gene ADH2, contains two finger domains in a region of the protein required for transcriptional activation6. Nineteen independently isolated adr1 mutations induced by hydroxylamine were found at nine different amino-acid positions, seven of which are in the two finger domains. All four mutations that altered invariant cysteine or histidine residues led to an adr1 null phenotype. Only one other mutation caused an adr1 null phenotype. Thus, one finger domain is not sufficient for ADR1 activity. This provides the first evidence that, as is consistent with the proposed model, the invariant cysteine and histidine residues are essential for the formation of the finger structure.

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Authors and Affiliations

  1. Department of Biochemistry SJ-70, University of Washington, Seattle, Washington, 98195, USA
    Hal Blumberg, Arri Eisen, Dorit Bader & Elton T. Young
  2. Zymosgenetics Inc., 2121 North 35th Street, Seattle, Washington, 98103, USA
    Andrzej Sledziewski

Authors

  1. Hal Blumberg
  2. Arri Eisen
  3. Andrzej Sledziewski
  4. Dorit Bader
  5. Elton T. Young

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Blumberg, H., Eisen, A., Sledziewski, A. et al. Two zinc fingers of a yeast regulatory protein shown by genetic evidence to be essential for its function.Nature 328, 443–445 (1987). https://doi.org/10.1038/328443a0

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