Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA (original) (raw)

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• Published: 24 September 1987

Nature volume 329, pages 341–343 (1987)Cite this article

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Abstract

E-cadherin is a cell surface glycoprotein responsible for Ca2+-dependent intercellular adhesion between epithelial cells1; it is also called uvomorulin2, L-CAM (ref. 3), cell-CAM 120/80 (ref. 4) or Arc-1 (ref. 5). Because blocking the action of E-cadherin by monoclonal antibodies causes dispersion of compact cell colonies1, this molecule is thought to be an important factor for maintenance of multicellular systems. To demonstrate directly that E-cadherin is involved in cell–cell adhesion, we cloned full-length cDNA encoding E-cadherin from F9 cells and introduced it into L fibroblasts deficient in E-cadherin. These L cells acquire strong Ca2+-dependent aggregating activity by expressing the E-cadherin derived from the introduced cDNA, and were morphologically transformed so as to form colonies in which cells were tightly connected to each other.

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Author notes

1. Masatoshi Takeichi: To whom correspondence should be addressed.

Authors and Affiliations

1. Department of Biophysics, Faculty of Science, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto, 606, Japan
   Akira Nagafuchi, Yasuaki Shirayoshi, Kenji Okazaki, Kunio Yasuda & Masatoshi Takeichi

Authors

1. Akira Nagafuchi
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2. Yasuaki Shirayoshi
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3. Kenji Okazaki
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4. Kunio Yasuda
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5. Masatoshi Takeichi
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Nagafuchi, A., Shirayoshi, Y., Okazaki, K. et al. Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA.Nature 329, 341–343 (1987). https://doi.org/10.1038/329341a0

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• Received: 17 June 1987
• Accepted: 04 August 1987
• Issue Date: 24 September 1987
• DOI: https://doi.org/10.1038/329341a0

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