Recovery of immunodeficient mice from a vaccinia virus/IL-2 recombinant infection (original) (raw)

Nature volume 329, pages 545–546 (1987)Cite this article

Abstract

Vaccinia virus recombinants that express cloned genes encoding antigens of unrelated infectious agents, such as hepatitis B virus1 and human immunodeficiency virus (HIV)2,3, provide a new approach to the development of live vaccines. Although there is evidence that genetically engineered vaccinia viruses have reduced pathogenicity4 a major obstacle to their use as vaccines is that severe complications can occur after vaccination, especially in immunodeficient individuals5,6. We describe here a recombinant vaccinia virus expressing murine interleukin-2 (IL-2) and show that athymic nude mice infected with the recombinant virus resolve the virus infection rapidly whereas mice infected with control virus develop a progressive vaccinal disease. By incorporating the gene for IL-2 in live virus vaccines it may be possible to prevent the severe complications that arise in recipients with an impaired immune system.

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References

  1. Smith, G. L., Mackett, M. & Moss, B. Nature 302, 490–495 (1983).
    Article ADS CAS Google Scholar
  2. Chakrabarti, S., Robert-Guroff, M., Wong-Staal, F., Gallo, R. C. & Moss, B. Nature 320, 535–537 (1986).
    Article ADS CAS Google Scholar
  3. Hu, S.-L., Kosowski, S. G. & Dalrymple, J. M. Nature 320, 537–540 (1986).
    Article ADS CAS Google Scholar
  4. Buller, R. M. L., Smith, G. L., Cremer, K., Notkins, A. L. & Moss, B. Nature 317, 813–815 (1985).
    Article ADS CAS Google Scholar
  5. Dick, G. Prog. med. Viro'l. 8, 1–29 (1966).
    CAS Google Scholar
  6. Redfield, R. R. et al. N. Engl. J. Med. 316, 673–676 (1987).
    Article CAS Google Scholar
  7. Smith, K. A. A. Rev. Immun. 2, 319–333 (1984).
    Article CAS Google Scholar
  8. Yokota, T. et al. Proc. natn. Acad. Sci. U.S.A 82, 68–72 (1985).
    Article ADS CAS Google Scholar
  9. Andrew, M. E., Coupar, B. E. H., Ada, G. L. & Boyle, D. B. Microbial Pathogenesis 1, 443–452 (1986).
    Article CAS Google Scholar
  10. Lifson, J. D., Benike, C. J., Mark, D. F., Koths, K. & Engleman, E. G. Lancet i, 698–702 (1984).
    Article Google Scholar
  11. Gluckman, J. C., Klatzmann, D. & Montagnier, L. A Rev. Immun. 4, 97–117 (1986).
    Article CAS Google Scholar
  12. Gillis, S., Ferm, M. M., Ou, W. & Smith, K. A. J. Immun. 120, 2027–2032 (1978).
    CAS Google Scholar
  13. Mosmann, T. J. immunol. Meth. 65, 55–63 (1983).
    Article CAS Google Scholar

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Authors and Affiliations

  1. Department of Microbiology, John Curtin School of Medical Research, Australian National University, GPO Box 334, Canberra, ACT, 2601, Australia
    Marion E. Andrew, Susan M. Phillips, David B. Boyle & Barbara E. H. Coupar
  2. Department of Experimental Pathology, John Curtin School of Medical Research, Australian National University, GPO Box 334, Canberra, ACT, 2601, Australia
    Ian A. Ramshaw
  3. CSIRO Australian Animal Health Laboratory, Geelong, Australia
    Marion E. Andrew, David B. Boyle & Barbara E. H. Coupar

Authors

  1. Ian A. Ramshaw
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  2. Marion E. Andrew
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  3. Susan M. Phillips
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  4. David B. Boyle
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  5. Barbara E. H. Coupar
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Ramshaw, I., Andrew, M., Phillips, S. et al. Recovery of immunodeficient mice from a vaccinia virus/IL-2 recombinant infection.Nature 329, 545–546 (1987). https://doi.org/10.1038/329545a0

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