Heparan sulphate bound growth factors: a mechanism for stromal cell mediated haemopoiesis (original) (raw)

Nature volume 332, pages 376–378 (1988)Cite this article

Abstract

The proliferation and development of haemopoietic stem cells takes place in close association with marrow stromal cells1,2. This intimate cell contact presumably enables the stem cells and their progeny to respond to stimuli present on the stromal cell surface. While the nature of these stimuli has not been determined, it is likely that growth factors play some role. Recently, it was demonstrated that the natural and the recombinant haemopoietic growth factor, granulocyte/macrophage colony stimulating factor (GM-CSF), could be adsorbed out of solution by an extract of human marrow stromal extracellular matrix (ECM) with retention of biological activity3. However, the precise ECM molecules involved were not identified. Here, we clearly demonstrate that the major sulphated glycosaminoglycan of mouse marrow stroma, heparan sulphate4, possesses the ability to adsorb both GM-CSF and the multilineage haemopoietic growth factor, Interleukin 3 (IL-3). Furthermore, these growth factors, once bound, can be presented in the biologically active form to haemopoietic cells.

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Authors and Affiliations

  1. Departments of Experimental Haematology Christie Hospital and Holt Radium Institute, Withington, Manchester, M20 9BX, UK
    R. Roberts, E. Spooncer, T. D. Alien, F. Bloomfield & T. M. Dexter
  2. Medical Oncology, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Withington, Manchester, M20 9BX, UK
    J. Gallagher

Authors

  1. R. Roberts
  2. J. Gallagher
  3. E. Spooncer
  4. T. D. Alien
  5. F. Bloomfield
  6. T. M. Dexter

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Roberts, R., Gallagher, J., Spooncer, E. et al. Heparan sulphate bound growth factors: a mechanism for stromal cell mediated haemopoiesis .Nature 332, 376–378 (1988). https://doi.org/10.1038/332376a0

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