Cytotoxic T cells specific for the circumsporozoite protein of Plasmodium falciparum (original) (raw)

Nature volume 334, pages 258–260 (1988)Cite this article

Abstract

Malaria is initiated by the inoculation of a susceptible host with sporozoites from an infected mosquito. The sporozoites enter hepatocytes and develop for a period as exoerythrocyte or hepatic stage parasites1. Vaccination with irradiated sporozoites can provide protective immunity1 and a recent study2 shows that this can also be conferred by immunization with a recombinant salmonella expressing only the circumsporozoite protein that normally covers the sporozoites. Protection against infection is likely to be mediated by cytotoxic CD8+ cells, as depletion of CD8+ T cells in a sporozoite-immunized animal can completely abrogate immunity3,4. Here we demonstrate directly the existence of CD8+ cytotoxic T lymphocytes (CTL) that recognize the circumsporozoite protein. B10.BR mice immunized with sporozoites or with recombinant vaccinia virus expressing the CS protein of Plasmodium falciparum contain CTL that specifically kill L cell fibroblasts transfected with the gene encoding the same CS protein. The peptide epitope from the CS protein that is recognized by CTL from this strain of mice is from a variant region of the protein.

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Authors and Affiliations

  1. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20892, USA
    Sanjai Kumar, Louis H. Miller, Isabella A. Quakyi, David B. Keister & Michael F. Good
  2. Biological Resources Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20892, USA
    W. Lee Maloy
  3. Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20892, USA
    Bernard Moss
  4. Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20892, USA
    Jay A. Berzofsky
  5. Department of Molecular Biology, Scripps Clinic and Research Foundation, La Jolla, California, 92037, USA
    Richard A. Houghten

Authors

  1. Sanjai Kumar
  2. Louis H. Miller
  3. Isabella A. Quakyi
  4. David B. Keister
  5. Richard A. Houghten
  6. W. Lee Maloy
  7. Bernard Moss
  8. Jay A. Berzofsky
  9. Michael F. Good

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Kumar, S., Miller, L., Quakyi, I. et al. Cytotoxic T cells specific for the circumsporozoite protein of Plasmodium falciparum.Nature 334, 258–260 (1988). https://doi.org/10.1038/334258a0

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