Extensive variation of human immunodeficiency virus type-1 in vivo (original) (raw)

Nature volume 334, pages 440–444 (1988)Cite this article

Abstract

Genotypic variation among independent isolates of human immunodeficiency virus type-1 (HIV-1) is well known1–9, but its molecular basis and biological consequences are poorly understood. We examined the genesis of molecular variation in HIV-1 by sequential virus isolations from two chronically infected individuals and analysis of recombinant HIV-1 genomic clones. In three different virus isolates full-length HIV-1 clones were identified and found to consist, respectively, of 17, 9 and 13 distinguishable, but highly-related, viral genotypes. Thirty-five viral clones derived from two HIV-1 isolates obtained from the same individual but 16 months apart showed progressive change, yet were clearly related. Similar changes in the HIV-1 genome did not occur in vitro during virus isolation and amplification. The results indicate that HIV-1 variation in vivo is rapid, that a remarkably large number of related but distinguishable genotypic variants evolve in parallel and coexist during chronic infection, and that 'isolates' of HIV-1, unless molecularly or biologically cloned, generally consist of complex mixtures of genotypically distinguishable viruses.

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Authors and Affiliations

  1. Department of Internal Medicine, University of Alabama at Birmingham, Birmingham, Alabama, 35294, USA
    Michael S. Saag, Beatrice H. Hahn, Joseph Gibbons, Yuexia Li & George M. Shaw
  2. Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, 35294, USA
    Beatrice H. Hahn & Yuexia Li
  3. Department of Biochemistry, University of Alabama at Birmingham, Birmingham, Alabama, 35294, USA
    George M. Shaw
  4. Department of Pediatrics, University of Miami School of Medicine, Miami, Florida, 33101, USA
    Elizabeth S. Parks & Wade P. Parks

Authors

  1. Michael S. Saag
  2. Beatrice H. Hahn
  3. Joseph Gibbons
  4. Yuexia Li
  5. Elizabeth S. Parks
  6. Wade P. Parks
  7. George M. Shaw

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Saag, M., Hahn, B., Gibbons, J. et al. Extensive variation of human immunodeficiency virus type-1 in vivo.Nature 334, 440–444 (1988). https://doi.org/10.1038/334440a0

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