Activation in vitro of sequence-specific DNA binding by a human regulatory factor (original) (raw)
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- Published: 22 September 1988
Nature volume 335, pages 372–375 (1988)Cite this article
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An Erratum to this article was published on 10 November 1988
Abstract
The human heat-shock factor (HSF) regulates heat-shock genes in response to elevated temperature1–6. When human cells are heated to 43 °C, HSF is modified post-translationally from a form that does not bind DNA to a form that binds to a specific sequence (the heat-shock element, HSE7,8) found upstream of heat-shock genes6. To investigate the transduction of the heat signal to HSF, and more generally, how mammalian cells respond at the molecular level to environmental stimuli, we have developed a cell-free system that exhibits heat-induced activation of human HSF in vitro. Comparison of HSF activation in vitro and in intact cells suggests that the response of human cells to heat shock involves at least two steps. First, an ATP-independent, heat-induced alteration of HSF allows it to bind the HSE; the temperature at which activation occurs in vitro implies that a human factor directly senses temperature. Second, HSF is phosphorylated. It is possible that similar multi-step activation mechanisms play a role in the response of eukaryotic cells to a variety of environmental stimuli, and that these mechanisms evolved to increase the range and flexibility of the response.
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References
- Ritossa, F. M. Experimenlia 18, 571–572 (1962).
Article CAS Google Scholar - Nover, L. Heal Shock Response of Eukaryolic Cells (Springer, Berlin, 1984).
Google Scholar - Craig, E. A. CRC Crit. Rev. Biochem. 18, 239–280 (1985).
Article CAS Google Scholar - Pelham, H. R. B. Trends Genet. 1, 31–35 (1985).
Article CAS Google Scholar - Lindquist, S. A. Rev. Biochem. 55, 1151–1191 (1986).
Article CAS Google Scholar - Kingston, R. E., Schuetz, T. J. & Larin, Z. Molec. cell. Biol. 7, 1530–1534 (1987).
Article CAS Google Scholar - Pelham, H. R. B. & Bienz, M. EMBO J. 1, 1473–1477 (1982).
Article CAS Google Scholar - Mirault, M.-E., Southgate, R. & Delwart, E. EMBO J. 1, 1279–1285 (1982).
Article CAS Google Scholar - Yamamoto, K. R. A. Rev. Genet. 19, 209–252 (1985).
Article CAS Google Scholar - Sen, R. & Baltimore, D. Cell 47, 921–928 (1986).
Article CAS Google Scholar - Prywes, R. & Roeder, R. G. Cell 47, 777–784 (1986).
Article CAS Google Scholar - Hayes, T. E., Kitchen, A. M. & Cochran, B. H. Proc. nat. Acad. Sci. U.S.A. 84, 1272–1276 (1987).
Article ADS CAS Google Scholar - Zimarino, V. & Wu, C. Nature 327, 727–730 (1987).
Article ADS CAS Google Scholar - Seguin, C. & Hamer, D. H. Science 235, 1383–1387 (1987).
Article ADS CAS Google Scholar - Baeuerle, P. A. & Baltimore, D. Cell 53, 211–217 (1988).
Article CAS Google Scholar - Sorger, P. K., Lewis, M. J. & Pelham, H. R. B. Nature 329, 81–84 (1987).
Article ADS CAS Google Scholar - Dignam, J. D., Lebovitz, R. M. & Roeder, R. G. Nucleic Acids Res. 11, 1475–1589 (1983).
Article CAS Google Scholar - Fried, M. & Crothcrs, D. M. Nucleic Acids Res. 9, 6505–6525 (1981).
Article CAS Google Scholar - Siebenlist, U. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 77, 122–126 (1980).
Article ADS CAS Google Scholar - Gilman, M. Z., Wilson, R. N. & Weinberg, R. A. Molec. cell. Biol. 6, 4305–4316 (1986).
Article CAS Google Scholar - Anantham, J., Goldberg, A. L. & Voellmy, R. Science 232, 522–524 (1986).
Article ADS Google Scholar - Finley, D., Ciechanover, A. & Varshavsky, A. Cell 37, 43–55 (1984).
Article CAS Google Scholar - Golf, S. A. & Goldberg, A. L. Cell 41, 587–595 (1985).
Article Google Scholar - Munro, S. & Pelham, H. Nature 317, 477–478 (1985).
Article ADS CAS Google Scholar - Wu, C. et al. Science 238, 1247–1253 (1987).
Article ADS CAS Google Scholar - Verjee, Z. H. M. Eur. J. Biochem. 9, 439–444 (1969).
Article CAS Google Scholar - Beckwith, J. & Zipser, D. The lactose operon (Cold Spring Harbor, New York, 1970).
Google Scholar - Wurm, F. M., Gwinn, K. A. & Kingston, R. E. Proc. natn. Acad. Sci. U.S.A. 83, 5415–5418 (1986).
Article ADS Google Scholar - Holmgren, R., Livak, K., Morimoto, R., Freund, R. & Meselson, M. Cell 18, 1359–1370 (1979).
Article CAS Google Scholar
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Authors and Affiliations
- Department of Genetics, Harvard Medical School and Department of Molecular Biology, Massachusetts General Hospital, Wellman 10, Boston, Massachusetts, 02114, USA
Jeffrey S. Larson, Thomas J. Schuetz & Robert E. Kingston
Authors
- Jeffrey S. Larson
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Larson, J., Schuetz, T. & Kingston, R. Activation in vitro of sequence-specific DNA binding by a human regulatory factor.Nature 335, 372–375 (1988). https://doi.org/10.1038/335372a0
- Received: 08 April 1988
- Accepted: 11 August 1988
- Issue Date: 22 September 1988
- DOI: https://doi.org/10.1038/335372a0