Positive selection of antigen-specific T cells in thymus by restricting MHC molecules (original) (raw)

Nature volume 335, pages 730–733 (1988)Cite this article

Abstract

Thymus-derived lymphocytes (T cells) recognize antigen in the context of class I or class II molecules encoded by the major histocompatibility complex (MHC) by virtue of the heterodimeric αβ T-cell receptor (TCR)1,2. CD4 and CDS molecules expressed on the surface of T cells bind to nonpolymorphic portions of class II and class I MHC molecules and assist the TCR in binding and possibly in signalling3,4. The analysis of T-cell development in TCR transgenic mice has shown that the CD4/CD8 phenotype of T cells is determined by the interaction of the αβ TCR expressed on immature CD4+8+ thymocytes with polymorphic domains of thymic MHC molecules in the absence of nominal antigen5. Here we provide direct evidence that positive selection of antigen-specific, class I MHC-restricted CD4−8+ T cells in the thymus requires the specific interaction of the αβ TCR with the restricting class I MHC molecule.

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References

  1. Dembic, Z. et al. Nature 320, 232–238 (1986).
    Article ADS CAS Google Scholar
  2. Saito, T. et al. Nature 325, 125–130 (1987).
    Article ADS CAS Google Scholar
  3. Gabert, J. et al. Cell 50, 545–554 (1987).
    Article CAS Google Scholar
  4. Gay, D. et al. Nature 328, 626–629 (1987).
    Article ADS CAS Google Scholar
  5. Teh, H. S. et al. Nature 335, 229–233 (1988).
    Article ADS CAS Google Scholar
  6. Kisielow, P. et al. Nature 333, 742–746 (1988).
    Article ADS CAS Google Scholar
  7. Staerz, U., Rammensee, H., Benedetto, J. & Bevan, M. J. Immun. 134, 3994–4000 (1985).
    CAS PubMed Google Scholar
  8. Bevan, M. J. Proc. natn. Acad. Sci. U.S.A. 74, 2094–2098 (1977).
    Article ADS CAS Google Scholar
  9. Lemmonier, F., Burakoff, S. J., Germain, R. N. & Benaceraff, B. Proc. natn. Acad. Sci. U.S.A. 74, 1229–1233 (1977).
    Article ADS Google Scholar
  10. Sredni, B. & Schwartz, R. H. Immun. Rev. 54, 187–223 (1981).
    Article CAS Google Scholar
  11. von Boehmer, H. et al. Eur. J. Immun. 9, 592–597 (1979).
    Article CAS Google Scholar
  12. Bevan, M. J. Nature 269, 417–419 (1977).
    Article ADS CAS Google Scholar
  13. Zinkernagel, R. M. et al. J. exp. Med. 147, 882–896 (1987).
    Article Google Scholar
  14. von Boehmer, H., Haas, W. & Jerne, N. K. Proc. natn. Acad. Sci. U.S.A. 75, 2434–2442 (1978).
    Article ADS Google Scholar
  15. Kappler, J. & Marrack, P. J. J. exp. Med. 148, 1510–1522 (1978).
    Article CAS Google Scholar
  16. Klein, J. in Immunology: the Science of Self-Non-Self Discrimination 267 (Wiley, New York, 1982).
    Google Scholar
  17. Matzinger, P. Nature 292, 497–499 (1981).
    Article ADS CAS Google Scholar
  18. Egorov, I. K., Mnatsakanyan & Pospelov, L. E. Immunogenetics 5, 65–74 (1977).
    Article Google Scholar
  19. Meo, T., Vives, J., Miggiano, V. & Shreffler, D. Trans. Proc. 5, 377–381 (1973).
    CAS Google Scholar
  20. Hünig, T. & Bevan, M. J. J. exp. Med. 155, 111–125 (1982).
    Article Google Scholar
  21. Marrack, P. & Kappler, J. Nature 332, 840–843 (1988).
    Article ADS CAS Google Scholar
  22. Teh, H. S., Bennink, J. & von Boehmer, H. Eur. J. Immun. 12, 887–892 (1982).
    Article CAS Google Scholar

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Authors and Affiliations

  1. Basel Institute for Immunology, 487 Grenzacherstrasse, CH-4058, Basel, Switzerland
    Pawel Kisielow, Hung Sia Teh & Harald von Boehmer
  2. Central Research Units, F. Hoffmann–La Roche & Co. Ltd, CH-4002, Basel, Switzerland
    Horst Blüthmann
  3. The Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
    Pawel Kisielow
  4. Department of Microbiology, University of British Columbia, Vancouver, V6T 1W5, Canada
    Hung Sia Teh

Authors

  1. Pawel Kisielow
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  2. Hung Sia Teh
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  3. Horst Blüthmann
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  4. Harald von Boehmer
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Kisielow, P., Teh, H., Blüthmann, H. et al. Positive selection of antigen-specific T cells in thymus by restricting MHC molecules.Nature 335, 730–733 (1988). https://doi.org/10.1038/335730a0

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