Induction of endogenous IFN-α and IFN-β genes by a regulatory transcription factor, IRF-1 (original) (raw)
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- Published: 19 January 1989
Nature volume 337, pages 270–272 (1989)Cite this article
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Abstract
Interferons (IFNs) have an important role in cell growth and differentiation. The most well-known function of IFNs is their antiviral activity; viral infections result in induction of the transcription of the IFN-α and IFN-β genes1–3. Recently we isolated the gene encoding a transcription factor, IRF-1, that may play a part in the induction of IFN genes4,5. Interestingly, the IRF-1 gene itself is virus-inducible, suggesting the importance of de novo production of IRF-1 in IFN gene induction5. Here we show that high-level expression of the cloned mouse IRF-1 gene in monkey COS cells results in the induction of endogeneous IFN-α and IFN-β genes without viral stimulation. Furthermore, we demonstrate the induction of these genes by an IRF-1/yeast GAL4 chimaeric transcription factor. This may be the first demonstration of the specific induction of silent chromosomal genes by transfection of a single transcription factor gene in mammalian cells.
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References
- Lengyel, P. A. Rev. Biochem. 51, 251–282 (1982).
Article CAS Google Scholar - Weissmann, C. & Weber, H. Nucleic Acids Res. 33, 251–300 (1986).
CAS Google Scholar - Taniguchi, T. A. Rev. Immun. 6, 439–464 (1988).
Article CAS Google Scholar - Fujita, T. et al. EMBO J. 7, 3397–3405 (1988).
Article CAS Google Scholar - Miyamoto, M. et al. Cell 54, 903–913 (1988).
Article CAS Google Scholar - Fujita, T., Shibuya, H., Hotta, H., Yamanishi, K. & Taniguchi, T. Cell 49, 357–367 (1987).
Article CAS Google Scholar - Adolf, G. J. gen. Virol. 68, 1669–1676 (1987).
Article CAS Google Scholar - Zinn, K., DiMaio, D. & Maniatis, T. Cell 34, 865–879 (1983).
Article CAS Google Scholar - Goodboum, S., Burstein, H. & Maniatis, T. Cell 45, 601–610 (1986).
Article Google Scholar - Kuhl, D. et al. Cell 50, 1057–1069 (1987).
Article CAS Google Scholar - Hiscott, J., Cantell, K. & Weissmann, C. Nucleic Acids Res. 12, 3727–3746 (1984).
Article CAS Google Scholar - Shuttleworth, J., Moser, J. & Burck, D. C. Eur. J. Biochem. 133, 399–404 (1983).
Article CAS Google Scholar - Seed, B. Nature 329, 840–842 (1988).
Article ADS Google Scholar - Ma, J. & Ptashne, M. Cell 48, 847–853 (1987).
Article CAS Google Scholar - Seed, B. & Aruffo, A. Proc. natn. Acad. Sci. U.S.A. 87, 3365–3369 (1987).
Article ADS Google Scholar - Hatakeyama, M. et al. Nature 318, 467–470 (1985).
Article ADS CAS Google Scholar - Wilson, V. et al. J. molec. Biol. 166, 457–475 (1983).
Article CAS Google Scholar - Fujita, T., Ohno, S., Yasumitsu, H. & Taniguchi, T. Cell 41, 489–496 (1985).
Article CAS Google Scholar - Mantei, N. et al. Gene 10, 1–10 (1980).
Article CAS Google Scholar
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Authors and Affiliations
- Institute for Molecular and Cellular Biology Osaka University, Suita-shi, Osaka, 565, Japan
Takashi Fujita, Yoko Kimura, Masaaki Miyamoto, Edward L. Barsoumian & Tadatsugu Taniguchi
Authors
- Takashi Fujita
You can also search for this author inPubMed Google Scholar - Yoko Kimura
You can also search for this author inPubMed Google Scholar - Masaaki Miyamoto
You can also search for this author inPubMed Google Scholar - Edward L. Barsoumian
You can also search for this author inPubMed Google Scholar - Tadatsugu Taniguchi
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Fujita, T., Kimura, Y., Miyamoto, M. et al. Induction of endogenous IFN-α and IFN-β genes by a regulatory transcription factor, IRF-1.Nature 337, 270–272 (1989). https://doi.org/10.1038/337270a0
- Received: 10 October 1988
- Accepted: 30 November 1988
- Issue Date: 19 January 1989
- DOI: https://doi.org/10.1038/337270a0