Characterization of a naturally processed MHC class II-restricted T-cell determinant of hen egg lysozyme (original) (raw)

Nature volume 342, pages 682–684 (1989)Cite this article

Abstract

COMPELLING evidence indicates that T cells recognize complexes formed by major histocompatibility complex-encoded molecules and antigenic peptide fragments1–3. This is based largely on the ability of small synthetic peptides to substitute for naturally processed antigen in stimulating T cells. Naturally processed fragments of exogenous antigen are thought to arise by limited pro-teolytic degradation of native antigen inside acidic compartments of antigen-presenting cells3–6, but until now no physiologically processed antigen has been directly analysed. Here we report the characterization of physiologically processed antigen eluted from mouse class II major histocompatibility complex I–Ed molecules. The antigenic material corresponds to a previously described antigenic determinant of hen egg lysozyme (HEL 107–116) and has a relative molecular mass _M_r of about 2,000. HPLC analysis identified at least two or three separate molecular species, suggesting limited, albeit significant, heterogeneity of naturally processed peptides. Finally, under our experimental conditions, it was calculated that a substantial proportion (10–40%) of I–Ed molecules were occupied by these HEL-derived antigenic determinants.

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References

  1. Shimonkevitz, R., Colon, S., Kappler, J. W., Marrack, P. & Grey, H. M. J. Immun. 133, 2067–2074 (1984).
    CAS PubMed Google Scholar
  2. Babbitt, B., Allen, P., Matsueda, G., Haber, E. & Unanue, E. Nature 317, 359–361 (1985).
    Article ADS CAS PubMed Google Scholar
  3. Buus, S. et al. Proc. natn. Acad. Sci. U.S.A. 83, 3968–3971 (1986).
    Article ADS CAS Google Scholar
  4. Chesnut, R. W., Colon, S. M. & Grey, H. M. J. Immun. 129, 2382–2387 (1982).
    CAS PubMed Google Scholar
  5. Ziegler, K. & Unanue, E. R. J. Immun. 127, 1869–1875 (1981).
    CAS PubMed Google Scholar
  6. Streicher, H. Z., Berkower, I. J., Busch, M., Gurd, F. R. N. & Berzofsky, J. Proc. natn. Acad. Sci. U.S.A. 81, 6831–6835 (1984).
    Article ADS CAS Google Scholar
  7. Adorini, L. et al. Proc. natn. Acad. Sci. U.S.A. 85, 5181–5185 (1988).
    Article ADS CAS Google Scholar
  8. Coeshott, C. M. et al. J. Immun. 136, 2832–2838 (1986).
    CAS PubMed Google Scholar
  9. Buus, S., Sette, A., Colon, S. M., Jenis, D. M. & Grey, H. M. Cell 47, 1071–1077 (1986).
    Article CAS PubMed Google Scholar
  10. Sette, A., Buus, S., Colon, S., Miles, C. & Grey, H. M. J. Immun. 142, 35–40 (1989).
    CAS PubMed Google Scholar
  11. Buus, S., Sette, A., Colon, S. M. & Grey, H. M. Science 242, 1045–1047 (1988).
    Article ADS CAS PubMed Google Scholar
  12. Falo, L. D. Jr., Benacerraf, B. & Rock, K. L. Proc. natn. Acad. Sci. U.S.A. 83, 6994–6997 (1986).
    Article ADS CAS Google Scholar

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Author notes

  1. E. Appella: National Cancer Institute, National Institutes of Health, Building 37, Room 1B04, Bethesda, Maryland 20892, USA

Authors and Affiliations

  1. Cytel, 11099 North Torrey Pines Road, La Jolla, California, 92037, USA
    S. Demotz, H. M. Grey, E. Appella & A. Sette

Authors

  1. S. Demotz
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  2. H. M. Grey
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  3. E. Appella
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  4. A. Sette
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Demotz, S., Grey, H., Appella, E. et al. Characterization of a naturally processed MHC class II-restricted T-cell determinant of hen egg lysozyme.Nature 342, 682–684 (1989). https://doi.org/10.1038/342682a0

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