The yeast SWI4 protein contains a motif present in developmental regulators and is part of a complex involved in cell-cycle-dependent transcription (original) (raw)

Nature volume 342, pages 830–833 (1989)Cite this article

Abstract

TRANSCRIPTION of the HO gene of Saccharomyces cerevisiae, which encodes a site-specific endonuclease that initiates cell-type switching (reviewed in refs. 1,2), is restricted to a short window of the cell cycle in late Gl (refs 3,4). A repeated element in the upstream region of HO (the cell-cycle box, CCB) and two regulatory proteins, SWI4 and SWI6, are required for cell-cycle-dependent expression of _HO_5,6. Biochemical experiments have identified a factor, CCBF (cell-cycle box factor), that binds to the CCB elements7 and that presumably plays a key part in cell-cycle regulation of HO. The SWI4 and SWI6 genes are required for formation of the CCBF-DNA complex. Here we report the nucleotide sequence of the SWI4 gene and show that it contains two copies of the conserved SWI6-cdclO motif observed in SWI6 of budding yeast8, the Schizosaccharomyces pombe cdc10 gene required for progression through G19, the Drosophila Notch gene, and in the Caenorhabditis elegans lin-12 and glp-1 genes10–12. We demonstrate by using antibodies to the SWI4 protein in gel-shift assays that the protein is present in the CCBF–DNA complex.

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References

  1. Nasmyth, K. A. & Shore, D. Science 237, 1162–1170 (1987).
    Article ADS CAS Google Scholar
  2. Herskowitz, I. Microbiol. Rev. 52, 536–553 (1988).
    CAS PubMed PubMed Central Google Scholar
  3. Jensen, R. E., Sprague, G. F. & Herskowitz, I. Proc. natn Acad Sci. U.S.A. 80, 3035–3039 (1983).
    Article ADS CAS Google Scholar
  4. Nasmyth, K. A. Nature 302, 670–676 (1983).
    Article ADS CAS Google Scholar
  5. Nasmyth, K. A. Cell 42, 225–235 (1985).
    Article CAS Google Scholar
  6. Breeden, L. & Nasmyth, K. A. Cell 48, 389–397 (1987).
    Article CAS Google Scholar
  7. Andrews, B. J. & Herskowitz, I. Cell 57, 21–29 (1989).
    Article CAS Google Scholar
  8. Breeden, L. & Nasmyth, K. Nature 329, 651–654 (1987).
    Article ADS CAS Google Scholar
  9. Aves, S. J., Durkacz, B. W., Carr, A. & Nurse, P. EMBO J. 4, 457–463 (1985).
    Article CAS Google Scholar
  10. Wharton, K. A., Johansen, K., Xu, T. & Artavanis-Tsakonas, S. Cell 43, 567–581 (1985).
    Article CAS Google Scholar
  11. Yochem, J., Weston, K. & Greenwald, I. Nature 335, 547–550 (1988).
    Article ADS CAS Google Scholar
  12. Yochem, J. & Greenwald, I. Cell 58, 887–898 (1989).
    Article Google Scholar
  13. Courey, A. J. & Tijan, R. Cell 55, 887–898 (1988).
    Article CAS Google Scholar
  14. Stern, M., Jensen, R. & Herskowitz, I. J. molec. Biol 178, 853–868 (1984).
    Article CAS Google Scholar
  15. Johnston, L. H., White, J. H. M., Johnson, A. L., Lucchini, G. & Plevani, P. Nucleic Acids Res. 15, 5017–5030 (1987).
    Article CAS Google Scholar
  16. Sternberg, P. W., Stern, M., Clark, I. & Herskowitz, I. Cell 48, 567–577 (1987).
    Article CAS Google Scholar
  17. Kleid, D., Yamsura, D., Small, B. & Dowbenko, D. Science 214, 1125–1128 (1981).
    Article ADS CAS Google Scholar
  18. Towbin, H., Staehelin, T. & Gordon, J. Proc. natn. Acad. Sci. U.S.A. 76, 4350–4354 (1979).
    Article ADS CAS Google Scholar
  19. Bender, A. & Sprague, G. F. Cell 50, 681–691 (1987).
    Article CAS Google Scholar
  20. Snyder, M. J. cell. Biol. 108, 1419–1429 (1989).
    Article CAS Google Scholar

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, 94143, USA
    Brenda J. Andrews & Ira Herskowitz

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  1. Brenda J. Andrews
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  2. Ira Herskowitz
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Andrews, B., Herskowitz, I. The yeast SWI4 protein contains a motif present in developmental regulators and is part of a complex involved in cell-cycle-dependent transcription.Nature 342, 830–833 (1989). https://doi.org/10.1038/342830a0

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