Phospholipid binding by a synaptic vesicle protein homologous to the regulatory region of protein kinase C (original) (raw)

Nature volume 345, pages 260–263 (1990)Cite this article

Abstract

NEUROTRANSMITTERS are released at synapses by the Ca2+-regulated exocytosis of synaptic vesicles, which are specialized secretory organelles that store high concentrations of neurotransmitters1,2. The rapid Ca2+-triggered fusion of synaptic vesicles is presumably mediated by specific proteins that must interact with Ca2+ and the phospholipid bilayer. We now report that the cytoplasmic domain of p65, a synaptic vesicle-specific protein3 that binds calmodulin4 contains an internally repeated sequence that is homologous to the regulatory C2-region of protein kinase C (PKC)5. The cytoplasmic domain of recombinant p65 binds acidic phospholipids with a specificity indicating an interaction of p65 with the hydrophobic core as well as the headgroups of the phospholipids. The binding specificity resembles PKC, except that p65 also binds calmodulin, placing the C2-regions in a context of potential Ca2+-regulation that is different from PKC. This is a novel homology between a cellular protein and the regulatory domain of protein kinase C. The structure and properties of p65 suggest that it may have a role in mediating membrane interactions during synaptic vesicle exocytosis.

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Authors and Affiliations

  1. Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas, 75235, USA
    Mark S. Perin, Gregory A. Mignery & Thomas C. Südhof
  2. Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas, 75235, USA
    Gregory A. Mignery & Thomas C. Südhof
  3. Department of Biochemistry, St Jude Children's Research Hospital, Memphis, Tennessee, 38101, USA
    Victor A. Fried
  4. MPI für Psychiatrie, Abteilung Neurochemie, 8033, Planegg-Martmsried, FRG
    Reinhard Jahn

Authors

  1. Mark S. Perin
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  2. Victor A. Fried
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  3. Gregory A. Mignery
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  4. Reinhard Jahn
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  5. Thomas C. Südhof
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Perin, M., Fried, V., Mignery, G. et al. Phospholipid binding by a synaptic vesicle protein homologous to the regulatory region of protein kinase C.Nature 345, 260–263 (1990). https://doi.org/10.1038/345260a0

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