Isolation and characterization of the gene for a yeast mitochondrial import receptor (original) (raw)

Nature volume 347, pages 488–491 (1990)Cite this article

Abstract

WE have previously identified an integral membrane protein (p32) from Saccharomyces cerevisiae as a receptor for protein import into mitochondria, and have localized it to the mitochondrial outer membrane at contact sites1. Here we report isolation of the corresponding mitochondrial import receptor gene, termed MIR1 The deduced amino-acid sequence of p32 shows roughly 40% identity with proteins of bovine heart2 and rat liver3 that have been suggested to be mitochondrial phosphate carriers. Haploid cells carrying a disrupted MIR1 allele were unable to grow on a non-fermentable carbon source but grew in media containing glucose, indicating that the MIR1 protein is essential for mitochondrial function. Compared with wild type, amounts of some mitochondrial proteins were markedly reduced in cells containing a disrupted MIR1 allele, whereas levels of others were unchanged. This indicates that yeast contains more than one pathway for protein import into mitochondria.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

References

  1. Pain, D., Murakami, H. & Blobel, G. Nature 347, 444–449 (1990).
    Article ADS CAS Google Scholar
  2. Runswick, M. J., Powell, S. J., Nyren, P. & Walker, J. E. EMBO J. 6, 1367–1373 (1987).
    Article CAS Google Scholar
  3. Ferreira, G. C., Pratt, R. D. & Pedersen, P. L. J. biol. Chem. 264, 15628–15633 (1989).
    CAS PubMed Google Scholar
  4. Green, M. R. A. Rev. Genet. 20, 671–708 (1986).
    Article CAS Google Scholar
  5. Hamilton, R., Watanabe, C. K. & de Boer, H. A. Nucleic Acids Res. 15, 3581–3593 (1987).
    Article CAS Google Scholar
  6. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).
    Article CAS Google Scholar
  7. Kaplan, R. S., Pratt, R. D. & Pedersen, P. L. Meth. Enzym. 173, 732–745 (1989).
    Article CAS Google Scholar
  8. Schulte, U. et al. Nature 339, 147–149 (1989).
    Article ADS CAS Google Scholar
  9. Brandriss, M. C. in Protein Transfer and Organelle Biogenesis (eds Das, R. C. & Robbins, P. W.) 655–676 (Academic, San Diego, 1988).
    Google Scholar
  10. Werner-Washburne, M., Stone, D. E. & Craig, E. A. Molec. cell. Biol. 7, 2568–2577 (1987).
    Article CAS Google Scholar
  11. Lawson, J. E. & Douglas, M. G. J. biol. Chem. 263, 14812–14818 (1988).
    CAS PubMed Google Scholar
  12. Maruyama, T., Gojobori, T., Aota, S. & Ikemura, T. Nucleic Acids Res. 14, r151–r197 (1986).
    Article CAS Google Scholar
  13. Mullis, K. B. & Faloona, F. A. Meth. Enzym. 155, 335–350 (1987).
    Article CAS Google Scholar
  14. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
    Article ADS CAS Google Scholar
  15. Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).
    Google Scholar
  16. Feinberg, A. P. & Vogelstein, B. Analyt. Biochem. 132, 6–13 (1983).
    Article CAS Google Scholar
  17. Feinberg, B. & MacLaughlin, C. S. in Yeast. A Practical Approach (eds Campbell, I. & Duffus, J. H.) 147–161 (IRL, Oxford 1988).
    Google Scholar
  18. Murakami, H., Pain, D. & Blobel, G. J. Cell Biol. 107, 2051–2057 (1988).
    Article CAS Google Scholar
  19. Sherman, F., Fink, G. R. & Hicks, J.B. Laboratory Course Manual for Methods in Yeast Genetics (Cold Spring Harbor Laboratory, New York, 1986).
    Google Scholar
  20. Lipman, D. J. & Pearson, W. R. Science 227, 1435–1441 (1985).
    Article ADS CAS Google Scholar
  21. Needleman, S. B. & Wunsch, C. D. J. molec. Biol. 48, 443–453 (1970).
    Article CAS Google Scholar
  22. Vieira, J. & Messing, J. Meth. Enzym. 153, 3–11 (1987).
    Article CAS Google Scholar
  23. Henikoff, S. Gene 28, 351–359 (1984).
    Article CAS Google Scholar
  24. Rothstein, R. J. Meth. Enzym. 101, 202–211 (1983).
    Article CAS Google Scholar

Download references

Author information

Authors and Affiliations

  1. Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York, 10021, USA
    Hiroshi Murakami, Günter Blobel & Debkumar Pain

Authors

  1. Hiroshi Murakami
    You can also search for this author inPubMed Google Scholar
  2. Günter Blobel
    You can also search for this author inPubMed Google Scholar
  3. Debkumar Pain
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Murakami, H., Blobel, G. & Pain, D. Isolation and characterization of the gene for a yeast mitochondrial import receptor.Nature 347, 488–491 (1990). https://doi.org/10.1038/347488a0

Download citation