Isolation and analysis of naturally processed viral peptides as recognized by cytotoxic T cells (original) (raw)

Nature volume 348, pages 252–254 (1990)Cite this article

Abstract

VIRUS-infected cells can be eliminated by cytotoxic T lymphocytes (CTL), which recognize virus-derived peptides bound to major histocompatibility complex (MHC) class I molecules on the cell surface1,2. Until now, this notion has relied on overwhelming but indirect evidence, as the existence of naturally processed viral peptides has not been previously reported. Here we show that such peptides can be extracted from virus-infected cells by acid elution. Both the naturally processed H–2-Db-restricted and H–2-Kd-restricted peptides from influenza nucleoprotein are smaller than the corresponding synthetic peptides, which have first been used to determine the respective CTL epitopes1,3. As with minor histocompatibility antigens4, occurrence of viral peptides seems to be heavily dependent on MHC class I molecules, because infected H–2d cells do not contain the H–2-Db-restricted peptide, and infected H–2b cells do not contain the H–2-Kd-restricted peptide. Our data provide direct experimental proof for the above notion on MHC-associated viral peptides on virus-infected cells.

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Author notes

  1. Karl Deres, Jörg Metzger and Günther Jung: Institut für Organische Chemie, Universität Tübingen,D-7400 Tübingen, Germany

Authors and Affiliations

  1. Max-Planck-lnstitut für Biologie, Abteilung Immungenetik, Corrensstrasse 42, D-7400, Tubingen, Germany
    Olaf Rötzschke, Kirsten Falk, Karl Deres, Hansjörg Schild, Maria Norda, Jörg Metzger, Günther Jung & Hans-Georg Rammensee

Authors

  1. Olaf Rötzschke
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  2. Kirsten Falk
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  3. Karl Deres
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  4. Hansjörg Schild
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  5. Maria Norda
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  6. Jörg Metzger
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  7. Günther Jung
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  8. Hans-Georg Rammensee
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Rötzschke, O., Falk, K., Deres, K. et al. Isolation and analysis of naturally processed viral peptides as recognized by cytotoxic T cells.Nature 348, 252–254 (1990). https://doi.org/10.1038/348252a0

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