Polarized sorting of glypiated proteins in hippocampal neurons (original) (raw)
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- Published: 10 January 1991
Nature volume 349, pages 158–161 (1991)Cite this article
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Abstract
OUR recent studies suggested that neurons and epithelial cells sort viral glycoproteins in a similar manner. The apical influenza virus haemagglutinin was preferentially delivered to the axon of hippocampal neurons in culture, whereas the basolateral vesicular stomatitis virus glycoprotein was sorted to the dendrites.1 To investigate whether other membrane proteins showed similar sorting in neurons and epithelial cells, we have analysed the localization of a glypiated (glycosylphosphatidylinositol-anchored) protein, Thy-1, in hippocampal neurons in culture. In MDCK and other epithelial cells, endogenous glycosylphosphatidylinositol (GPI)-anchored proteins, as well as mutated exogenous proteins containing the GPI-attachment signal, undergo preferential delivery to the apical surface2–4. This polarized sorting of GPI-anchored proteins has been proposed to occur by the same mechanisms as the sorting of glycolipids to the apical surface5,6. We report here that the neuronal GPI-protein Thy-1 is present in hippocampal neurons in culture and is exclusively located on the axonal surface. This finding further strengthens our hypothesis that the mechanisms of sorting of surface components may be similar in neurons and epithelial cells.
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References
- Dotti, C. & Simons, K. Cell 62, 63–72 (1990).
Article CAS Google Scholar - Lisanti, M., Sargiacomo, M., Graeve, C., Saltiel, A. & Roderiguez-Boulan, E. Proc. natn. Acad. Sci. U.S.A. 85, 9557–9561 (1988).
Article ADS CAS Google Scholar - Lisanti, M., Caras, J., Davitz, M. & Rodriguez-Boulan, E. J. Cell Biol. 109, 2140–2156 (1989).
Google Scholar - Wilson, J., Fasel, N. & Kraehenbuhl, J-P. J. Cell Sci. 96, 143–149 (1990).
CAS PubMed Google Scholar - Lisanti, M. & Roderiguez-Boulan, E. Trends Biochem. Sci. 15, 113–118 (1990).
Article CAS Google Scholar - Simons, K. & Wandinger-Ness, A. Cell 62, 207–210 (1990).
Article CAS Google Scholar - Conzelmann, A., Spiazzi, A. & Bron, C. Biochem. J. 246, 605–610 (1989).
Article Google Scholar - Dotti, C., Sullivan, C. & Banker, G. J. Neurosci. 8, 1454–1468 (1988).
Article CAS Google Scholar - Banker, G. & Waxman, A. In Intrinsic Determinants of Neuronal Form and Function (ed. Lesek, R. J.) 61–82 (Alan Liss, New York, 1988).
Google Scholar - Cáceres, A., Banker, G., Steward, O., Binder, L. & Payne, M. Devl Brain Res. 13, 314–318 (1988).
Article Google Scholar - Bartlett, W. & Banker, G. J. Neurosci. 4, 1954–1965 (1984).
Article CAS Google Scholar - Rothman, S. & Cowan, W. J. comp. Neurol. 195, 141–155 (1981).
Article CAS Google Scholar - Simons, K. & van Meer, G. Biochemistry 27, 6197–6202 (1988).
Article CAS Google Scholar - Gumbiner, B. Am. J. Physiol. 253, C749–C758 (1987).
Article CAS Google Scholar - Ishihara, D., Hou, Y. & Jacobson, K. Proc. natn. Acad. Sci. U.S.A. 84, 1290–1293 (1987).
Article ADS CAS Google Scholar - Bennett, M., Wandinger-Ness, A. & Simons, K. EMBO J. 7, 4075–4085 (1988).
Article CAS Google Scholar - Laemmli, V. Nature 257, 418–420 (1979).
Google Scholar - Towbin, H., Straehelin, T. & Gordon, J. Proc. natn. Acad. Sci. U.S.A. 76, 4350–4354 (1979).
Article ADS CAS Google Scholar - Mason, D. & Williams, A. Biochem. J. 147, 1–20 (1980).
Article Google Scholar - Morris, R., Beech, J., Barber, P. & Raisman, G. J. Neurocytol. 14, 427–452 (1985).
Article CAS Google Scholar
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Authors and Affiliations
- Cell Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-6900, Heidelberg, Germany
Carlos G. Dotti, Robert G. Parton & Kai Simons
Authors
- Carlos G. Dotti
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Dotti, C., Parton, R. & Simons, K. Polarized sorting of glypiated proteins in hippocampal neurons.Nature 349, 158–161 (1991). https://doi.org/10.1038/349158a0
- Received: 28 August 1990
- Accepted: 01 November 1990
- Issue Date: 10 January 1991
- DOI: https://doi.org/10.1038/349158a0