Bioorganic synthesis of lipid-modified proteins for the study of signal transduction (original) (raw)
References
Resh, M. D. Regulation of cellular signalling by fatty acid acylation and prenylation of signal transduction proteins. Cell. Signal.8, 403–412 (1996). ArticleCAS Google Scholar
Wittinghofer, A. Signal transduction via Ras. Biol. Chem.379, 933–973 (1998). CAS Google Scholar
Gelb, M. H. Protein prenylation, et cetera: signal transduction in two dimensions. Science275, 1750–1751 (1997). ArticleCAS Google Scholar
Waddick, K. G. & Uckun, F. M. Innovative treatment programs against cancer. I. Ras oncoprotein as a molecular target. Biochem. Pharmacol.56, 1411–1426 (1998). ArticleCAS Google Scholar
Page, M. J. et al. Expression and characterization of the Ha-ras p21 protein produced at high levels in the insect/baculovirus system. J. Biol. Chem.264, 19147–19154 (1989). CASPubMed Google Scholar
Schmittberger, T., Cotté, A. & Waldmann, H. Synthesis of characteristic lipopeptides of lipid modified proteins employing the allylester as protecting group. Chem. Comm., 937–938 (1998).
Nagele, E., Schelhaas, M., Kuder, N. & Waldmann, H. Chemoenzymatic synthesis of N-Ras lipopeptides. J. Am. Chem. Soc.120, 6889–6902 (1998). Article Google Scholar
Tucker, J. et al. Expression of p21 proteins in Escherichia coli and stereochemistry of the nucleotide-binding site. EMBO J.5, 1351–1358 (1986). ArticleCAS Google Scholar
Bordier, C. Phase separation of integral membrane proteins in Triton X-114 solution. J. Biol. Chem.256, 1604–1607 (1981). CASPubMed Google Scholar
Kalb, E., Frey, S. & Tamm, L. K. Formation of supported planar bilayers by fusion of vesicles to supported phospholipid monolayers. Biochim. Biophys. Acta1103, 307–316 (1992). ArticleCAS Google Scholar
Schelhaas, M. et al. Chemoenzymatic synthesis of biotinylated ras peptides and their use in membrane binding studies of lipidated model proteins by surface plasmon resonance. Chem. Eur. J.5, 1239–1252 (1999). ArticleCAS Google Scholar
Herrmann, C., Martin, G. A. & Wittinghofer, A. Quantitative analysis of the complex between p21ras and the Ras-binding domain of the human Raf-1 protein kianse. J. Biol. Chem.270, 2901–2905 (1995). ArticleCAS Google Scholar
Lenzen, C., Cool, R. H., Prinz, H., Kuhlmann, J. & Wittinghofer, A. Kinetic analysis by fluorescence of the interaction between Ras and the catalytic domain of the guanine nucleotide exchange factor Cdc25Mm. Biochemistry37, 7420–7930 (1998). ArticleCAS Google Scholar
Bar-Sagi, D. & Feramisco, J. R. Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation. Cell42, 841–848 (1985). ArticleCAS Google Scholar
Schmidt, G. et al. Biochemical and biological consequences of changing the specificity of p21ras from guanosine to xanthosine nucleotides. Oncogene12, 87–96 (1996). CASPubMed Google Scholar
Willumsen, B. M., Cox, A. D., Solski, P. A., Der, C. J. & Buss, J. E. Novel determinants of H-Ras plasma membrane localization and transformation. Oncogene13, 1901–1909 (1996). CASPubMed Google Scholar
Siddiqui, A. A., Garland, J. R., Dalton, M. B. & Sinensky, M. Evidence for a high affinity, saturable, prenylation-dependent p21Ha-ras binding site in plasma membranes. J. Biol. Chem.273, 3712–3717 (1998). ArticleCAS Google Scholar
Shahinian, S. & Silvius, J. R. Doubly-lipid-modified protein sequence motifs exhibit long-lived anchorage to lipid bilayer membranes. Biochemistry34, 3813–3822 (1995). ArticleCAS Google Scholar
MacDonald, R. C. et al. Small-volume extrusion apparatus for preparation of large, unilamellar vesicles. Biochim. Biophys. Acta1061, 297–303 (1991). ArticleCAS Google Scholar
Mayer, L. D., Hope, M. J. & Cullis, P. R. Vesicles of variable sizes produced by a rapid extrusion procedure. Biochim. Biophys. Acta858, 161–168 (1986). ArticleCAS Google Scholar
Schelhaas, M., Glomsda, S., Hänsler, M., Jakubke, H. D. & Waldmann, H. Enzymatic synthesis of peptides and Ras lipopeptides employing choline ester as a solubilizing, protecting, and activating group. Angew. Chem. Int. Edn Engl.35, 106–109 (1996). ArticleCAS Google Scholar