Cofilin/ADF is required for cell motility during Drosophila ovary development and oogenesis (original) (raw)
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- Published: 18 January 2001
Nature Cell Biology volume 3, pages 204–209 (2001)Cite this article
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Abstract
The driving force behind cell motility is the actin cytoskeleton. Filopodia and lamellipodia are formed by the polymerization and extension of actin filaments towards the cell membrane1,2. This polymerization at the barbed end of the filament is balanced by depolymerization at the pointed end, recycling the actin in a 'treadmilling' process2,3. One protein involved in this process is cofilin/actin-depolymerizing factor (ADF), which can depolymerize actin filaments, allowing treadmilling to occur at an accelerated rate3,4. Cofilin/ADF is an actin-binding protein that is required for actin-filament disassembly, cytokinesis and the organization of muscle actin filaments4,5,6,7. There is also evidence that cofilin/ADF enhances cell motility3,8,9, although a direct requirement in vivo has not yet been shown. Here we show that Drosophila cofilin/ADF6,10, which is encoded by the twinstar (tsr) gene, promotes cell movements during ovary development and oogenesis. During larval development, cofilin/ADF is required for the cell rearrangement needed for formation of terminal filaments, stacks of somatic cells that are important for the initiation of ovarioles. It is also required for the migration of border cells during oogenesis. These results show that cofilin/ADF is an important regulator of actin-based cell motility during Drosophila development.
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Acknowledgements
We thank H. D. Pham for excellent technical assistance, J. Pendelton, J. Peredo and T. Tran for their assistance in screening the 1,800 P-element lethal lines, and M. Bejar, J. Monroy and L. Ng for help with mapping tsr ntf. We also thank T. Orr-Weaver, P. Morcillo, the Bloomington Stock Center and T. Laverty (Berkeley Drosophila Genome Project) for stocks, and U. Tepass for critical reading of the manuscript. This work was supported by USPHS National Research Service Awards GM07185 and GM07617 (to J.C. and K.G., respectively). D.G. was supported by the Natural Science and Engineering Research Council of Canada. F.A.L. and M.G. were supported by NIH grants GM40451 and GM48430, respectively.
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Authors and Affiliations
- Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, 90095, California, USA
Jiong Chen & Frank A. Laski - Molecular Biology Institute, University of California at Los Angeles, Los Angeles, 90095 , California, USA
Frank A. Laski - Department of Zoology, University of Toronto, Toronto, M5S 3G5, Ontario, Canada
Dorothea Godt - Department of Molecular Biology and Genetics, Cornell University, Ithaca, 14853, New York, USA
Kris Gunsalus & Michael Goldberg - Institute of Genetics, Hungarian Academy of Sciences , Szeged, H-6701, Hungary
Istvan Kiss
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- Jiong Chen
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Correspondence toFrank A. Laski.
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Chen, J., Godt, D., Gunsalus, K. et al. Cofilin/ADF is required for cell motility during Drosophila ovary development and oogenesis.Nat Cell Biol 3, 204–209 (2001). https://doi.org/10.1038/35055120
- Received: 08 December 1999
- Revised: 07 August 2000
- Accepted: 09 October 2000
- Published: 18 January 2001
- Issue Date: February 2001
- DOI: https://doi.org/10.1038/35055120