Independent inactivation of MPF and cytostatic factor (Mos) upon fertilization of Xenopus eggs (original) (raw)
- Letter
- Published: 18 July 1991
Nature volume 352, pages 247–248 (1991)Cite this article
- 209 Accesses
- 161 Citations
- 3 Altmetric
- Metrics details
Abstract
IN vertebrates, mature eggs are arrested at the second meiotic metaphase by the cytostatic factor (CSF)1, now known to be the_c-_ mos proto-oncogene product (Mos)2, 3. Fertilization or egg activaá-tion triggers a transient increase in the cytoplasmic free calcium4, 5 and releases the meiotic arrest by inactivating maturation/mitosis-promoting factor (MPF)6, 7. CSF or Mos, which is also inactivated by the calcium transient8, 9, seems to stabilize MPF in mature eggs and CSF-injected embryos2, 6, 10. Thus, it was assumed that CSF inactivation is the primary cause of MPF inactivation on meiotic release2, 6, 8, 10–14. We have directly compared the degradation kinetics of CSF (Mos) and MPF during meiotic release, using the same batch of Xenopus eggs. We report here that, at the molecular level, cyclin subunits of MPF are degraded before Mos is degraded and, at the physiological level, that MPF activity is inactivated before CSF activity during activation of Xenopus eggs. These results, in conjunction with circumstantial evidence, support the novel view that a calcium transient on fertilization induces a CSF-independent pathway for MPF inactivation, whereas CSF inactivation during meiotic release serves only to allow the fertilá-ized egg to enter mitosis.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
References
- Masui, Y. & Markert, C. L. J. exp. Zool. 177, 129–146 (1971).
Article CAS Google Scholar - Sagata, N., Watanabe, N., Vande Woude, G. F. & Ikawa, Y. Nature 342, 512–518 (1989).
Article ADS CAS Google Scholar - Okazaki, K. et al. Jap. J. Cancer Res. 82, 250–253 (1991).
Article CAS Google Scholar - Busa, W. B. & Nuccitelli, R. J. Cell Biol. 100, 1325–1329 (1989).
Article Google Scholar - Kubota, H. Y., Yoshimoto, Y., Yoneda, M. & Hiramoto, Y. Devl Biol. 119, 129–136 (1987).
Article CAS Google Scholar - Newport, J. W. & Kirschner, M. W. Cell 37, 731–742 (1984).
Article CAS Google Scholar - Murray, A. W. & Kirschner, M. W. Nature 339, 275–280 (1989).
Article ADS CAS Google Scholar - Meyerhof, P. G. & Masui, Y. Devl Biol. 61, 214–229 (1977).
Article CAS Google Scholar - Watanabe, N., Vande Woude, G. F., Ikawa, Y. & Sagata, N. Nature 342, 505–511 (1989).
Article ADS CAS Google Scholar - Gerhart, J., Wu, M. & Kirschner, M. J. Cell Biol. 98, 1247–1255 (1984).
Article CAS Google Scholar - Murray, A. W., Solomon, M. J. & Kirschner, M. W. Nature 339, 280–286 (1989).
Article ADS CAS Google Scholar - Hunt, T. Nature 342, 483–484 (1989).
Article ADS CAS Google Scholar - Roy, L. M. et al. Cell 61, 825–831 (1990).
Article CAS Google Scholar - Lorca, T. et al. Molec. cell. Biol. 11, 1171–1175 (1991).
Article CAS Google Scholar - Gautier, J. et al. Cell 60, 487–494 (1990).
Article CAS Google Scholar - Minshull, J., Golsteyn, R., Hill, C. S. & Hunt, T. EMBO J. 9, 2865–2875 (1990).
Article CAS Google Scholar - Sagata, N., Oskarsson, M., Copeland, T., Brumbaugh, J. & Vande Woude, G. F. Nature 335, 519–525 (1988).
Article ADS CAS Google Scholar - Freeman, R. S., Kanki, J. P., Ballantyne, S. M., Pickham, K. M. & Donoghue, D. J. J. Cell Biol. 111, 533–541 (1990).
Article CAS Google Scholar - Yew, N., Oskarsson, M., Daar, I., Blair, D. G. & Vande Woude, G. F. Molec. cell. Biol. 11, 604–610 (1991).
Article CAS Google Scholar - Shibuya, E. K. & Masui, Y. Devl Biol. 129, 253–264 (1988).
Article CAS Google Scholar - Karsenti, E., Newport, J., Hubble, R. & Kirschner, M. J. Cell Biol. 98, 1730–1745 (1984).
Article CAS Google Scholar - Glotzer, M., Murray, A. W. & Kirschner, M. W. Nature 349, 132–138 (1991).
Article ADS CAS Google Scholar - Studier, F. W. & Moffatt, B. A. J. molec. Biol. 189, 113–130 (1986).
Article CAS Google Scholar - Sagata, N., Daar, I., Oskarsson, M., Showalter, S. D. & Vande Woude, G. F. Science 245, 643–646 (1989).
Article ADS CAS Google Scholar
Author information
Authors and Affiliations
- Tsukuba Life Science Centre, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, Ibaraki, 305, Japan
Nobumoto Watanabe & Yoji Ikawa - ICRF Clare Hall Laboratories, South Mimms, Hertfordshire, EN6 3LD, UK
Tim Hunt - Tokyo Medical and Dental University, Yushima, Bunkyo, Tokyo, 113, Japan
Yoji Ikawa - Division of Molecular Genetics, Institute of Life Science, Kurume University, 2432-3 Aikawa, Kurume, Fukuoka, 830, Japan
Noriyuki Sagata
Authors
- Nobumoto Watanabe
You can also search for this author inPubMed Google Scholar - Tim Hunt
You can also search for this author inPubMed Google Scholar - Yoji Ikawa
You can also search for this author inPubMed Google Scholar - Noriyuki Sagata
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Watanabe, N., Hunt, T., Ikawa, Y. et al. Independent inactivation of MPF and cytostatic factor (Mos) upon fertilization of Xenopus eggs.Nature 352, 247–248 (1991). https://doi.org/10.1038/352247a0
- Received: 24 April 1991
- Accepted: 10 June 1991
- Issue Date: 18 July 1991
- DOI: https://doi.org/10.1038/352247a0