MutT protein specifically hydrolyses a potent mutagenic substrate for DNA synthesis (original) (raw)
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- Published: 16 January 1992
Nature volume 355, pages 273–275 (1992)Cite this article
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Abstract
ERRORS in the replication of DNA are a major source of spontaneous mutations, and a number of cellular functions are involved in correction of these errors to keep the frequency of spontaneous mutations very low1. We report here a novel mechanism which prevents replicational errors by degrading a potent mutagenic substrate for DNA synthesis. This error-avoiding process is catalysed by a protein encoded by the_mutT_ gene of Escherichia coli, mutations of which increase the occurrence of A · T-→ C · G transversions 100 to 10,000 times the level of the wild type2. Spontaneous oxidation of dGTP forms 8-oxo-7,8-dihydro-2'-dGTP (8-oxodGTP), which is inserted opposite dA and dC residues of template DNA with almost equal efficiency, and the MutT protein specifically degrades 8-oxodGTP to the monophosphate. This indicates that elimination from the nucleotide pool of the oxidized form of guanine nucleotide is important for the high fidelity of DNA synthesis.
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References
- Horiuchi, T., Maki, H. & Sekiguchi, M. Bull. Inst. Pasteur 87, 309–336 (1989).
CAS Google Scholar - Yanofsky, C., Cox, E. C. & Horn, V. Proc. natn. Acad. Sci. U.S.A. 55, 274–281 (1966).
Article ADS CAS Google Scholar - Akiyama, M., Maki, H., Sekiguchi, M. & Horiuchi, T. Proc. natn. Acad. Sci. U.S.A. 86, 3949–3952 (1989).
Article ADS CAS Google Scholar - Bhatnagar, S. K. & Bessman, M. J. biol. Chem. 263, 8953–8957 (1988).
CAS PubMed Google Scholar - Kasai, H. & Nishimura, S. Nucleic Acids Res. 12, 2137–2145 (1984).
Article CAS Google Scholar - Kasai, H. & Nishimura, S. in Oxidative Stress: Oxidants and Antioxidants (ed. Sies, H.), 99–116 (Academic, London, 1991).
Google Scholar - Shibutani, S., Takeshita, M. & Grollman, A. P. Nature 349, 431–434 (1991).
Article ADS CAS Google Scholar - Maki, H. & Kornberg, A. J. biol. Chem. 260, 12987–12992 (1985).
CAS PubMed Google Scholar - Sloane, D. L., Goodman, M. F. & Echols, H. Nucleic Acids Res. 16, 6465–6475 (1988).
Article CAS Google Scholar - Kasai, H., Tanooka, H. & Nishimura, S. Gann 75, 1037–1039 (1984).
CAS PubMed Google Scholar - Wood, M. L., Dizdaroglu, M., Gajewski, E. & Essigman, J. M. Biochemistry 29, 7024–7032 (1990).
Article CAS Google Scholar - Moriya, M. et al. Mutation Res. 254, 281–288 (1991).
Article CAS Google Scholar - Tchou, J. et al. Proc. natn. Acad. Sci. U.S.A. 88, 4690–4694 (1991).
Article ADS CAS Google Scholar - Cabrera, M., Nghiem, Y. & Miller, J. H. J. Bact. 170, 5405–5407 (1988).
Article CAS Google Scholar - Michaels, M. L., Pham, L., Cruz, C. & Miller, J. Nucleic Acids Res. 19, 3629–3632 (1988).
Article Google Scholar - Boosalis, M. S., Petruska, J. & Goodman, M. F. J. biol. Chem. 262, 14689–14696 (1987).
CAS PubMed Google Scholar - Fersht, A. in Enzyme Structure and Mechanism 91–92 (Freeman, San Francisco, 1977).
Google Scholar - Akiyama, M., Horiuchi, T. & Sekiguchi, M. Molec. gen. Genet. 206, 9–16 (1987).
Article CAS Google Scholar - Bradford, M. M. Analyt. Biochem. 72, 248–254 (1976).
Article CAS Google Scholar
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Authors and Affiliations
- Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka, 812, Japan
Hisaji Maki & Mutsuo Sekiguchi
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- Hisaji Maki
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Maki, H., Sekiguchi, M. MutT protein specifically hydrolyses a potent mutagenic substrate for DNA synthesis.Nature 355, 273–275 (1992). https://doi.org/10.1038/355273a0
- Received: 11 September 1991
- Accepted: 14 October 1991
- Issue Date: 16 January 1992
- DOI: https://doi.org/10.1038/355273a0