Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation (original) (raw)

Nature volume 362, pages 175–179 (1993)Cite this article

Abstract

BASAL transcription by human RNA polymerase II requires the coordinate action of several ancillary factors (TFIIA-J)1 and can be regulated by various promoter-specific DNA binding proteins. An additional class of factors, called coactivators, are dispensable for basal transcription but are indispensable for regulation by transcriptional activators2–4. Biochemical studies established that some coactivators are associated with the TATA-binding protein (TBP) to form the TFIID complex3–6. We therefore set out to define the relationship between TBP and these TBP-associated factors (TAFs). Here we describe the cloning, expression and properties of the first human TAF, hTAFII250. The hTAFII250 gene is identical to a gene, CCG1 (refs 7, 8), implicated in cell-cycle progression. Recombinant hTAFII250 binds directly to TBP both in vitro and in yeast, and participates in the formation of the TFIID complex. This largest TAF may therefore play a central role in TFIID assembly by interacting with both TBP and other TAFs, as well as serving to link the control of transcription to the cell cycle.

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References

  1. Zawel, L. & Reinberg, D. Prog. Nucl. Acids Res. molec. Biol. 44, 67–108 (1993).
    Article CAS Google Scholar
  2. Pugh, B. F. & Tjian, R. Cell 61, 1187–1197 (1990).
    Article CAS Google Scholar
  3. Dyniacht, B. D., Hoey, T. & Tjian, R. Cell 55, 563–576 (1991).
    Article Google Scholar
  4. Tanese, N., Pugh, B. F. & Tjian, R. Genes Dev. 5, 2212–2224 (1991).
    Article CAS Google Scholar
  5. Pugh, B. F. & Tjian, R. Genes Dev. 5, 1935–1945 (1991).
    Article CAS Google Scholar
  6. Hoey, T. et al. Cell 72, 1–20 (1993).
    Article Google Scholar
  7. Sekiguchi, T., Miyata, T. & Nishimoto, T. EMBO J. 7, 1683–1687 (1988).
    Article CAS Google Scholar
  8. Sekiguchi, T., Nohiro, Y., Nakamura, Y., Hisamoto, N. & Nishimoto, T. Molec. cell. Biol. 11, 3317–3325 (1991).
    Article CAS Google Scholar
  9. Talavera, A. & Basilico, C. J. cell. Physiol. 92, 425–436 (1977).
    Article CAS Google Scholar
  10. Fields, S. & Song, O. Nature 340, 245–246 (1989).
    Article ADS CAS Google Scholar
  11. Nikolov, D. B. et al. Nature 360, 40–46 (1992).
    Article ADS CAS Google Scholar
  12. Liu, H. T. et al. J. biol. Chem. 260, 3269–3274 (1985).
    CAS PubMed Google Scholar
  13. Zhou, Q., Liebermann, P. M., Boyer, T. G. & Berk, A.-J. Genes Dev. 6, 1964–1974 (1992).
    Article CAS Google Scholar
  14. Kaelin, W. G. et al. Cell 70, 351–364 (1992).
    Article CAS Google Scholar
  15. Jantzen, H.-M., Chow, A. M., King, D. S. & Tjian, R. Genes Dev. 6, 1950–1963 (1992).
    Article CAS Google Scholar
  16. Harlow, E. & Lane, D. Antibodies. A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, 1988).
    Google Scholar
  17. Ruppert, S., Cole, T. J., Boshart, M., Schmid, E. & Schuetz, G. EMBO J. 11, 1503–1512 (1992).
    Article CAS Google Scholar
  18. Short, J. M., Fernandez, J. M., Sorge, J. A. & Huse, W. D. Nucleic Acids Res. 16, 7583–7600 (1988).
    Article CAS Google Scholar
  19. Field, J. et al. Molec. cell. Biol. 8, 2159–2165 (1988).
    Article CAS Google Scholar
  20. Peterson, M. G., Tanese, N., Pugh, B. F. & Tjian, R. Science 248, 1625–1630 (1990).
    Article ADS CAS Google Scholar
  21. Dynlacht, B. D., Attardi, L. D., Admon, A., Freeman, M. & Tjian, R. Genes Dev. 3, 1677–1688 (1989).
    Article CAS Google Scholar
  22. Chien, C.-T., Bartel, P. L., Sternglanz, R. & Fields, S. Proc. natn. Acad. Sci. U.S.A. 88, 9578–9582 (1991).
    Article ADS CAS Google Scholar

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  1. Howard Hughes Medical Institute, Department of Molecular and Cell Biology, 401 Barker Hall, University of California at Berkeley, Berkeley, California, 94720, USA
    Siegfried Ruppert, Edith H. Wang & Robert Tjian

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  1. Siegfried Ruppert
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  2. Edith H. Wang
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  3. Robert Tjian
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Ruppert, S., Wang, E. & Tjian, R. Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation.Nature 362, 175–179 (1993). https://doi.org/10.1038/362175a0

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