Tumour-suppressor activity of H19 RNA (original) (raw)
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- Published: 21 October 1993
Nature volume 365, pages 764–767 (1993)Cite this article
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Abstract
LOSS of heterozygosity in certain human embryonal tumours implicates a tumour-suppressor gene at chromosome 11p15.5 and selective loss of maternal alleles suggests that this gene is paternally imprinted1–4. The human HI9 gene maps to 11p1S.5, is expressed in differentiating fetal cells5–11 and is paternally imprinted12–16. We report here that two embryonal tumour cell lines, RD and G401, showed growth retardation and morphological changes when transfected with an H19 expression construct. More importantly, clonogenicity in soft agar and tumorigenicity in nude mice were abrogated in the G401-H19 transfectants. In addition to demonstrating its tumour-suppressor potential, this transfection system should help structural and functional studies of the enigmatic H19 gene.
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- Benjamin Tycko: To whom correspondence should be addressed.
Authors and Affiliations
- Divisions of Oncology and Neuropathology, Department of Pathology, Department of Pediatrics, Columbia University College of Physicians and Surgeons, New York, New York, 10032, USA
Yue Hao, Taria Crenshaw & Benjamin Tycko - Division of Pediatric Hematology-Oncology, Department of Pediatrics, Columbia University College of Physicians and Surgeons, New York, New York, 10032, USA
Thomas Moulton - Department of Pathology, New York University School of Medicine, New York, New York, 10016, USA
Elizabeth Newcomb
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- Yue Hao
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Hao, Y., Crenshaw, T., Moulton, T. et al. Tumour-suppressor activity of H19 RNA.Nature 365, 764–767 (1993). https://doi.org/10.1038/365764a0
- Received: 16 June 1993
- Accepted: 17 August 1993
- Issue Date: 21 October 1993
- DOI: https://doi.org/10.1038/365764a0