Defective mitosis due to a mutation in the gene for a fission yeast 26S protease subunit (original) (raw)

Nature volume 366, pages 355–357 (1993)Cite this article

Abstract

WE have isolated a mutant, mts2, in the fission yeast_Schizosaccharomyces pombe_ which is defective in chromosome segregation. The predicted amino-acid sequence of the cloned mts2+ gene product is 75% identical to the S4 subunit of the human 26S ATP/ubiquitin-dependent protease1. The human S4 subunit complementary DNA expressed from an S. pombe expression plasmid can rescue an S. pombe mts2 gene disruption. Both observations demonstrate that the mts2+ gene is the S. pombe_homologue of the human S4 subunit. In addition, we provide genetic evidence for a physical interaction between the S4 and the related S7 subunit in the 26S multiprotein protease. We show that polyubiquitin-conjugated proteins accumulate in the_mts2_mutant at the restrictive temperature, demonstrating that the mutant has an_in vivo_defect in the ubiquitin-dependent proteolysis pathway. Finally, the phenotype for the_mts2 mutant indicates that protein degradation by the 26S protease is essential not for entry into but for the completion of mitosis.

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Author notes

  1. Patrick Dillon and Craig Rosen: Department of Gene Regulation, Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110-1199, USA
  2. Patrick Dillon and Craig Rosen: Human Genome Sciences, 9620 Medical Center Drive, Rockville, Maryland 20850, USA

Authors and Affiliations

  1. MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh, EH4 2XU, UK
    Colin Gordon, Gordon McGurk, Patrick Dillon, Craig Rosen & Nicholas D. Hastie

Authors

  1. Colin Gordon
  2. Gordon McGurk
  3. Patrick Dillon
  4. Craig Rosen
  5. Nicholas D. Hastie

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Gordon, C., McGurk, G., Dillon, P. et al. Defective mitosis due to a mutation in the gene for a fission yeast 26S protease subunit.Nature 366, 355–357 (1993). https://doi.org/10.1038/366355a0

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