A role for Rac in Tiaml-induced membrane ruffling and invasion (original) (raw)
- Letter
- Published: 25 May 1995
- Gaston G. M. Habets1,
- Jord C. Stam1,
- Rob A. van der Kammen1 &
- …
- John G. Collard1 na1
Nature volume 375, pages 338–340 (1995)Cite this article
- 895 Accesses
- 6 Altmetric
- Metrics details
Abstract
RHO-LIKE GTPases have been implicated in the regulation of the actin cytoskeleton which controls the morphology, adhesion and motility of cells1-3. Like Ras proteins, they become activated when bound GDP is exchanged for GTP, a process catalysed by GDP-dissociation stimulator (GDS) proteins4. Several GDS proteins specific for Rho-like GTPases have been identified5-8. Most of these contain a conserved catalytic domain, the DBL-homology (DH) domain9, and activate Cdc42 or Rho but not Rac5-8. We have isolated the invasion-inducing Tiaml gene, which also encodes a protein with a DH domain10. Here we show that Tiaml is a GDS protein for Rho-like GTPases in vitro. In fibroblasts, Tiaml induces a similar phenotype as constitutively activated (Vll)Racl, including membrane ruffling, and this is inhibited by dominant negative (N17)Racl. Moreover, T-lymphoma cells expressing V12Racl become invasive, indicating that the Tiaml-Rae signalling pathway could be operating in the invasion and metastasis of tumour cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
References
- Hall, A. Molec. Biol. Cell 3, 475–479 (1992).
Article CAS Google Scholar - Tominaga, T. et al. J. Cell Biol. 120, 1529–1537 (1993).
Article CAS Google Scholar - Takaishi, K. et al. Molec. cell. Biol. 13, 72–79 (1993).
Article CAS Google Scholar - Boguski, M. S. & McCormick, F. Nature 366, 643–654 (1993).
Article ADS CAS Google Scholar - Hiraoka, K. et al. Biochem. biophys. Res. Commun. 182, 921–930 (1992).
Article CAS Google Scholar - Hart, M. J. et al. Nature 354, 311–314 (1991).
Article ADS CAS Google Scholar - Zheng, Y., Cerione, R. & Bender, A. J. biol. Chem. 269, 2369–2372 (1994).
CAS PubMed Google Scholar - Horii, Y. et al. EMBO J. 13, 4776–4786 (1994).
Article CAS Google Scholar - Hart, M. J. et al. J. biol. Chem. 269, 62–65 (1994).
CAS PubMed Google Scholar - Habets, G. G. M. et al. Cell 77, 537–549 (1994).
Article CAS Google Scholar - Chuang, T. H., Xu, X. M., Quilliam, L. A. & Bokoch, G. M. Biochemical J. 303, 761–767 (1994).
Article CAS Google Scholar - Shinjo, K. et al. Proc. natn. Acad. Sci. U.S.A. 87, 9853–9857 (1990).
Article ADS CAS Google Scholar - Ridley, A. J. & Hall, A. Cell 70, 389–399 (1992).
Article CAS Google Scholar - Ridley, A. J., Paterson, H., Johnston, C., Diekman, D. & Hall, A. Cell 70, 401–410 (1992).
Article CAS Google Scholar - Sekine, A., Fujiwara, M. & Narumiya, S. J. biol. Chem. 264, 8602–8605 (1989).
CAS PubMed Google Scholar - Nishiyama, T. et al. Molec. cell. Biol. 14, 2447–2456 (1994).
Article CAS Google Scholar - Nobes, C. & Hall, A. Curr. Opin. Genet. Dev. 4, 77–81 (1994).
Article CAS Google Scholar - Cook, S. & McCormick, F. Nature 369, 361–362 (1994).
Article ADS CAS Google Scholar - Habets, G. G. M., van der Kammen, R. A., Stam, J. C., Michiels, F. & Collard, J. G. Oncogene 10, 1371–1376 (1995).
CAS PubMed Google Scholar - Bokoch, G. M. Curr. Opin. Cell Biol. 6, 212–218 (1994).
Article CAS Google Scholar - Qiu, R-G., Chen, J., Kirn, D., McCormick, F. & Symons, M. Nature 374, 457–459 (1995).
Article ADS CAS Google Scholar - Evan, G. I. et al. Molec. cell. Biol. 5, 3610–3616 (1985).
Article CAS Google Scholar - Smith, D. B. & Johnson, K. S. Gene 67, 31–40 (1988).
Article CAS Google Scholar - Collard, J. G., Schijven, J. F. & Roos, E. Cancer Res. 47, 754–759 (1987).
CAS PubMed Google Scholar - Dranoff, G. et al. Proc. natn. Acad. Sci. U.S.A. 90, 3539–3543 (1993).
Article ADS CAS Google Scholar - Pear, W. S. et al. Proc. natn. Acad. Sci. U.S.A. 90, 8392–8396 (1993).
Article ADS CAS Google Scholar
Author information
Author notes
- John G. Collard: To whom correspondence should be addressed.
Authors and Affiliations
- Division of Cell Biology, The Netherlands Cancer Institute, 121 Plesmanlaan, 1066 CX, Amsterdam, The Netherlands
Frits Michiels, Gaston G. M. Habets, Jord C. Stam, Rob A. van der Kammen & John G. Collard
Authors
- Frits Michiels
You can also search for this author inPubMed Google Scholar - Gaston G. M. Habets
You can also search for this author inPubMed Google Scholar - Jord C. Stam
You can also search for this author inPubMed Google Scholar - Rob A. van der Kammen
You can also search for this author inPubMed Google Scholar - John G. Collard
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Michiels, F., Habets, G., Stam, J. et al. A role for Rac in Tiaml-induced membrane ruffling and invasion.Nature 375, 338–340 (1995). https://doi.org/10.1038/375338a0
- Received: 28 December 1994
- Accepted: 29 March 1995
- Issue Date: 25 May 1995
- DOI: https://doi.org/10.1038/375338a0