Identification of a specific lns(l,3,4,5)P4-binding protein as a member of the GAP1 family (original) (raw)
- Letter
- Published: 10 August 1995
- J. Justin Hsuan3,
- Oanh Truong3,
- Andrew J. Letcher1,
- Trevor R. Jackson4,
- Alan P. Dawson2 &
- …
- Robin F. Irvine1
Nature volume 376, pages 527–530 (1995)Cite this article
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Abstract
INOSITOL 1,3,4,5-tetrakisphosphate (Ins(l,3,4,5)P4) is produced rapidly from inositol 1,4,5-trisphosphate (Ins(l,4,5)P3) in stimulated cells1,2. Despite extensive experimentation, no clearly defined cellular function has yet been described for this inositol phosphate. Binding sites specific for Ins(l,3,4,5)P4 have been identified in several tissues3,4, and we have purified one such protein to homogeneity5. Its high affinity for Ins(l,3,4,5)P4, and its exquisite specificity for this isomeric configuration5,6, suggest it may be an Ins(l,3,4,5)P4 receptor. Here we report the cloning and characterization of this protein as a GTPase-activating protein, specifically a member of the GAP1 family. In vitro it shows GAP activity against both Rap and Ras, but only the Ras GAP activity is inhibited by phospholipids and is specifically stimulated by Ins(l,3,4,5)P4.
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References
- Irvine, R. F. in Inositol Phosphates and Calcium Signalling (ed. Putney, J. W. Jr.) 161–185 (Raven Press, New York, 1992).
Google Scholar - Batty, I. R., Nahorski, S. R. & Irvine, R. F. Biochem. J. 232, 211–215 (1985).
Article CAS Google Scholar - Cullen, P. J. & Irvine, R. F. Biochem. J. 288, 149–154 (1992).
Article CAS Google Scholar - Irvine, R. F. & Cullen, P. J. Curr. Biol. 3, 540–543 (1993).
Article CAS Google Scholar - Cullen, P. J. Dawson, A. P. & Irvine, R. F. Biochem. J. 305, 139–143 (1995).
Article CAS Google Scholar - Cullen, P. J. Chung, S.-K., Chang Y-T., Dawson, A. P. & Irvine, R. F. FEBS Lett. 358, 240–243 (1995).
Article CAS Google Scholar - Gaul, U., Mardon, G. & Rubin, G. M. Cell 68, 1007–1019 (1992).
Article CAS Google Scholar - Maekawa, M. et al. Molec. cell. Biol. 14, 6879–6885 (1994).
Article CAS Google Scholar - Gibson, T. J., Hyvonen, M., Musacchio, A., Saraste, M. & Birney, E. Trends biol. Sci. 19, 349–353 (1994).
Article CAS Google Scholar - Pawson, T. Nature 373, 573–580 (1995).
Article ADS CAS Google Scholar - Cullen, P. J., Patel, Y., Kakkar, V. V., Irvine, R. F. & Authi, K. S. Biochem J. 298, 739–742 (1994).
Article CAS Google Scholar - Maekawa, M., Nakamura, S. & Hattori, S. J. biol. Chem. 268, 22948–22952 (1993).
CAS PubMed Google Scholar - Bender, A. Proc. natn. Acad. Sci. U.S.A. 90, 9926–9929 (1993).
Article ADS CAS Google Scholar - Park, H. O., Chant, J. & Herskowitz, I. Nature 365, 269–274 (1993).
Article ADS CAS Google Scholar - Fukuda, M., Aruga, J., Niinoke, M., Aimoto, S. & Mikishiba, K. J. biol. Chem. 269, 29206–29211 (1994).
CAS PubMed Google Scholar - Ramirez, M. T., Post, G. R., Sulakhe, P. V. & Brown, J. H. J. biol. Chem. 270, 8846–8451 (1995).
Article Google Scholar - Manning, S. D. & Brass, L. F. Thrombos. Haemost. 66, 393–399 (1991).
Article CAS Google Scholar - Corvazier, E. et al. Biochem. J. 281, 323–331 (1991).
Google Scholar - Hashii, M., Nozawa, Y. & Higashida, H. J. biol. Chem. 268, 19403–19410 (1993).
CAS PubMed Google Scholar - Totty, N. F., Waterfield, M. D. & Hsuan, J. J. Prot. Sci. 1, 1215–1224 (1992).
Article CAS Google Scholar - Kozak, M. Nucleic Acids Res. 15, 8125–8148 (1987).
Article CAS Google Scholar
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Authors and Affiliations
- Inositide Laboratory, The Babraham Institute, Babraham Hall, Cambridge, CB2 4AT, UK
Peter J. Cullen, Andrew J. Letcher & Robin F. Irvine - School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
Peter J. Cullen & Alan P. Dawson - Ludwig Institute of Cancer Research, University College London Medical School, 91 Riding House Street, London, W1P 8BT, UK
J. Justin Hsuan & Oanh Truong - The Babraham Institute, Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, Cambridge, CB2 3EJ, UK
Trevor R. Jackson
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Cullen, P., Hsuan, J., Truong, O. et al. Identification of a specific lns(l,3,4,5)P4-binding protein as a member of the GAP1 family.Nature 376, 527–530 (1995). https://doi.org/10.1038/376527a0
- Received: 21 April 1995
- Accepted: 15 June 1995
- Issue Date: 10 August 1995
- DOI: https://doi.org/10.1038/376527a0