Organ targeting In vivo using phage display peptide libraries (original) (raw)

Nature volume 380, pages 364–366 (1996)Cite this article

Abstract

PREFERENTIAL homing of tumour cells1,2 and leukocytes3,4 to specific organs indicates that tissues carry unique marker molecules accessible to circulating cells. Organ-selective address molecules on endothelial surfaces have been identified for lymphocyte homing to various lymphoid organs and to tissues undergoing inflammation5–8, and an endothelial marker responsible for tumour homing to the lungs has also been identified9. Here we report a new approach to studying organ-selective targeting based on _in vivo_screening of random peptide sequences. Peptides capable of mediating selective localization of phage to brain and kidney blood vessels were identified, and showed up to 13-fold selectivity for these organs. One of the peptides displayed by the brain-localizing phage was synthesized and shown to specifically inhibit the localization of the homologous phage into the brain. When coated onto glutaraldehyde-fixed red blood cells, the peptide caused selective localization of intravenously injected cells into the brain. These peptide sequences represent the first step towards identifying selective endothelial markers, which may be useful in targeting cells, drugs and genes into selected tissues.

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Authors and Affiliations

  1. La Jolla Cancer Research Center, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, California, 92037, USA
    Renata Pasqualini & Erkki Ruoslahti

Authors

  1. Renata Pasqualini
  2. Erkki Ruoslahti

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Pasqualini, R., Ruoslahti, E. Organ targeting In vivo using phage display peptide libraries.Nature 380, 364–366 (1996). https://doi.org/10.1038/380364a0

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