Selective modulation of protein kinase C-Θ during T-cell activation (original) (raw)

Nature volume 385, pages 83–86 (1997)Cite this article

Abstract

EVERY cell contains many families of protein kinases, and may express several structurally related yet genetically distinct kinases of each family. The activity of the serine/threonine protein kinase C (PKC) enzymes1–2 has long been implicated in T-cell activation3, but it is not known which members of the PKC family regulate the T-cell response to foreign antigens. The activation of T cells by antigen-presenting cells (APCs) is spatially restricted to their site of contact, where receptors on the T cells engage their counter-receptors on the APCs4,5. We used this localized engagement to identify, at the single-cell level, intracel-lular proteins involved in the activation process. By digital immunofluorescence microscopy, we localized six isoforms of PKC in antigen-specific T-cell clones activated by APCs. Surprisingly, only PKC-Θ translocated to the site of cell contact. Accordingly, in vitro kinase activity assays of PKC immunoprecipitates from the conjugates of T cells and APCs showed a selective increase in the activity of PKC-Θ, indicating that the translocated enzyme is active. Several modes of partial T-cell activation that failed to cause PKC-Θ translocation also failed to cause T-cell proliferation, further suggesting the involvement of PKC-Θ in T-cell activation.

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Authors and Affiliations

  1. Division of Basic Sciences, National Jewish Center for Immunology, 1400 Jackson Street, Denver, Colorado, 80206, USA
    Colin R. F. Monks, Hannah Kupfer, Idan Tamir & Abraham Kupfer
  2. Department of Cellular and Structural Biology, University of Colorado Health Sciences Center, Denver, Colorado, 80262, USA
    Colin R. F. Monks & Abraham Kupfer
  3. Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut, 06510, USA
    Avlin Barlow

Authors

  1. Colin R. F. Monks
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  2. Hannah Kupfer
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  3. Idan Tamir
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  4. Avlin Barlow
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  5. Abraham Kupfer
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Monks, C., Kupfer, H., Tamir, I. et al. Selective modulation of protein kinase C-Θ during T-cell activation.Nature 385, 83–86 (1997). https://doi.org/10.1038/385083a0

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