Regulation of telomere length and function by a Myb-domain protein in fission yeast (original) (raw)
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- Published: 20 February 1997
Nature volume 385, pages 744–747 (1997)Cite this article
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Abstract
Telomeres, the specialized nucleoprotein structures that comprise the ends of eukaryotic chromosomes1,2, are essential for complete replication3–5, and regulation of their length has been a focus of research on tumorigenesis6–8. In the budding yeast Saccharomyces cerevisiae, the protein Raplp binds to telomeric DNA and functions in the regulation of telomere length9–12. A human telomere protein, hTRF (human TTAGGG repeat factor) binds the telomere sequence _in vitro_13 and localizes to telomeres cytologically14, but its functions are not yet known. Here we use a genetic screen to identify a telomere protein in fission yeast, Tazlp (telomere-associated in Schizosaccharomyces pombe), that shares homology to the Myb proto-oncogene DNA-binding domain with hTRF. Disruption or deletion of the taz1+ gene causes a massive increase in telomere length. Tazlp is required for the repression of telomere-adjacent gene expression and for normal meiosis or sporulation. It may be a negative regulator of the telomere-replicating enzyme, telomerase1,3, or may protect against activation of telomerase-independent pathways of telomere elongation8.
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Author notes
- Julia Promisel Cooper
Present address: Imperial Cancer Research Fund, Lincoln's Inn Fields, London, WC2A 3PX, UK
Authors and Affiliations
- Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado, 80309, USA
Julia Promisel Cooper & Thomas R. Cech - MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh, EH4 2XU, UK
Elaine R. Nimmo & Robin C. Allshire
Authors
- Julia Promisel Cooper
You can also search for this author inPubMed Google Scholar - Elaine R. Nimmo
You can also search for this author inPubMed Google Scholar - Robin C. Allshire
You can also search for this author inPubMed Google Scholar - Thomas R. Cech
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Cooper, J., Nimmo, E., Allshire, R. et al. Regulation of telomere length and function by a Myb-domain protein in fission yeast.Nature 385, 744–747 (1997). https://doi.org/10.1038/385744a0
- Received: 28 August 1996
- Accepted: 23 January 1997
- Issue Date: 20 February 1997
- DOI: https://doi.org/10.1038/385744a0
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