RNA interference in adult mice (original) (raw)

Gene expression

Nature volume 418, pages 38–39 (2002)Cite this article

Abstract

RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo.

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Figure 1: RNA interference in adult mice.

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Authors and Affiliations

  1. Departments of Pediatrics and Genetics, Stanford University School of Medicine, Stanford, 94305-5208, California, USA
    Anton P. McCaffrey, Leonard Meuse, Thu-Thao T. Pham & Mark A. Kay
  2. Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, 11724, New York, USA
    Douglas S. Conklin & Gregory J. Hannon

Authors

  1. Anton P. McCaffrey
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  2. Leonard Meuse
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  3. Thu-Thao T. Pham
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  4. Douglas S. Conklin
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  5. Gregory J. Hannon
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  6. Mark A. Kay
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Corresponding author

Correspondence toMark A. Kay.

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The authors declare no competing financial interests.

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McCaffrey, A., Meuse, L., Pham, TT. et al. RNA interference in adult mice.Nature 418, 38–39 (2002). https://doi.org/10.1038/418038a

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