Transgenic expression of green fluorescence protein can cause dilated cardiomyopathy (original) (raw)

Nature Medicine volume 6, pages 482–483 (2000)Cite this article

To the editor

A common approach for determining the molecular mechanisms of cardiac hypertrophy and failure has been the use of transgenic mice1. Many transgenic models of dilated cardiac hypertrophy have been reported2. In these models, the non-transgenic littermates are generally used as controls. However, because non-specific effects of a transgene can be dose- and time-dependent, non-transgenic littermates might not be a sufficient control for transgenic experiments.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 12 print issues and online access

$209.00 per year

only $17.42 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Figure 1: Confirmation of GFP expression by western blot analysis, and cardiac pathology.

References

  1. James, J.F., Hewett T.E. & Robbins, J. Cardiac physiology in transgenic mice. Circ. Res. 82, 407–415 ( 1998).
    Article CAS Google Scholar
  2. Kadambi, V. I. & Kranias, E. G. Genetically engineered mice: model systems for left ventricular failure. J. Card. Fail. 4, 349–361 (1998).
    Article CAS Google Scholar
  3. Heim, R. Prasher, D.C. & Tsien, R.Y. Wavelength mutations and posttranslational autoxidation of green fluorescence protein. Proc. Natl. Acad. Sci. USA 91, 12501–12504 (1994).
    Article CAS Google Scholar
  4. Godwin,A.R. et al. Detection of targeted GFP-Hox gene fusions during mouse embryogenesis . Proc. Natl. Acad. Sci. USA 95, 13042– 13047 (1998).
    Article CAS Google Scholar
  5. Subramaniam, A. et al. Tissue-specific regulation of the alpha-myosin heavy chain gene promoter in transgenic mice. J. Biol. Chem. 266 , 24613–24620 (1991).
    CAS PubMed Google Scholar
  6. Okabe, M. et al. Green mice as a source of ubiquitous green cells. FEBS Lett. 407, 313–319 (1997).
    Article CAS Google Scholar

Download references

Author information

Authors and Affiliations

  1. Cardiovascular division, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, 02215, Massachusetts , USA
    Weei-Yuarn Huang, Pamela S. Douglas & Seigo Izumo
  2. The Center for Blood Research, 200 Longwood Avenue, Boston, 02215, Massachusetts, USA
    Jose Aramburu

Authors

  1. Weei-Yuarn Huang
    You can also search for this author inPubMed Google Scholar
  2. Jose Aramburu
    You can also search for this author inPubMed Google Scholar
  3. Pamela S. Douglas
    You can also search for this author inPubMed Google Scholar
  4. Seigo Izumo
    You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toSeigo Izumo.

Rights and permissions

About this article

Cite this article

Huang, WY., Aramburu, J., Douglas, P. et al. Transgenic expression of green fluorescence protein can cause dilated cardiomyopathy.Nat Med 6, 482–483 (2000). https://doi.org/10.1038/74914

Download citation