Endocytic vesicles move at the tips of actin tails in cultured mast cells (original) (raw)
- Brief Communication
- Published: May 1999
- Stephen E. Moss1,
- Christoph Ballestrem2,
- Beat A. Imhof2,
- Günter Giese3,
- Ilse Wunderlich3 &
- …
- Wolfhard Almers3 nAff4
Nature Cell Biology volume 1, pages 72–74 (1999)Cite this article
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Actin polymerization is thought to provide the motive force for crawling cells by driving the spread of lamellipodia 1. It may also be involved when membrane ruffles engulf external fluid during macropinocytosis 2. Here we show that, in cells transfected with a fusion protein consisting of green fluorescent protein (GFP) and β-actin, pinosomes (vesicles used for fluid uptake) ignite a burst of actin polymerization when they are pinched off from the plasma membrane. Pinosomes then move into the cytosol at the tips of short-lived actin ‘comet tails’ that are similar to those that propel Listeria 3 and other microorganisms 4,5 through infected cells. Like Listeria, pinosomes appear to carry the machinery required for propulsive actin polymerization. The direction of pinosome movement indicates that they may acquire this machinery from the crests of membrane ruffles. We suggest that actin polymerization moves the leading edge of ruffles. Endocytic vesicles may also use actin polymerization to move into the cytosol after being pinched off from the plasma membrane.
The results shown in Fig. 1a were recorded by evanescent field fluorescence microscopy (EFM) 8, a technique that selectively images the bottom of cells where they adhere to a glass coverslip. Some actin tails seemed to originate in the middle of the cell in such recordings; such tails were also seen when the bottom of chemically fixed cells was viewed by laser-scanning confocal microscopy (LSCM). However, three-dimensional images of such tails reconstructed from serial confocal sections showed them to extend to the plasma membrane above (data not shown). In four cells analysed in this way, the appearance of all tails was consistent with the tails having begun at the plasma membrane.
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Acknowledgements
This work was supported by the Schweizerische Krebsliga and the Swiss National Science Foundation, by a European Commission grant and by the Max Planck Society. C.J.M. was the recipient of an EMBO short-term fellowship. We thank T. Soldati and T. Lang for comments on the manuscript.
Correspondence and requests for materials should be addressed to W. A.
Supplementary information is available on _Nature Cell Biology_’s World-Wide Web site (http://www.nature.com/ncb/webfocus/index.html).
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- Christien J. Merrifield & Wolfhard Almers
Present address: Vollum Institute, 3181 Sam Jackson Park Road, Portland, Oregon, 97201-3098, USA
Authors and Affiliations
- Department of Physiology, University College London, Gower Street, London , WC1E 6BT, UK
Christien J. Merrifield & Stephen E. Moss - Department of Pathology, Centre Medical Universitaire, Rue Michel-Servet 1, Geneva, CH-1211, Switzerland
Christoph Ballestrem & Beat A. Imhof - Max Planck Institute für Medizinische Forschung, Jahnstrasse 29, Heidelberg, 69120 , Germany
Günter Giese, Ilse Wunderlich & Wolfhard Almers
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Merrifield, C., Moss, S., Ballestrem, C. et al. Endocytic vesicles move at the tips of actin tails in cultured mast cells .Nat Cell Biol 1, 72–74 (1999). https://doi.org/10.1038/9048
- Received: 25 February 1999
- Revised: 06 April 1999
- Accepted: 06 April 1999
- Issue Date: May 1999
- DOI: https://doi.org/10.1038/9048