Asian Journal of Andrology (original) (raw)

Volume 11, Issue 5 (September 2009) 11, 541–547; 10.1038/aja.2009.53

Expression of TRPC6 in benign and malignant human prostate tissues

Dan Yue1, Yong Wang2, Jian-Ying Xiao3, Ping Wang2 and Chang-Shan Ren1

1 Cancer Research Institute, First Affiliated Hospital, China Medical University, Shenyang 110001, China
2 Department of Urology, Fourth Affiliated Hospital, China Medical University, Shenyang 110032, China
3 Department of Biochemistry, Liaoning Medical University, Jinzhou 121001, China

Correspondence: Dr Chang-Shan Ren,csrencmu@yahoo.com.cn

Received 3 June 2009; Revised 13 July 2009; Accepted 29 July 2009; Published online 24 August 2009.

| Abstract | | | | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ---------------------------- | | | We investigated the expression of transient receptor potential canonical 6 (TRPC6) protein in benign and malignant human prostate tissues and in prostate cancer cell lines and the association with the stage, grade and androgen responsiveness of the tumors. Immunohistochemical techniques, Western blot and reverse transcription polymerase chain reaction (RT-PCR) were used to investigate TRPC6 expression. TRPC6 protein was detected in 9 of 20 (45.0%) of benign prostatic hyperplasia (BPH) cases, and there was a significant difference compared with prostate cancer (129 of 149 [86.6%])(P < 0.01). TRPC6 expression was associated with the histological grade and extraprostatic extension (P < 0.01). Tumors of higher stage tended to have a higher frequency of TRPC6 protein staining, but the difference was not significant among T2, T3 and T4. TRPC6 expression difference between androgen-independent (AI) tumors and androgen-dependent (AD) tumors was not statistically significant. TRPC6 was also observed in prostate cancer cell lines. In summary, TRPC6 is detected in benign and malignant human prostate tissues and prostate cancer cell lines and is associated with the histological grade, Gleason score and extraprostatic extension of prostate cancer.Keywords: benign prostatic hyperplasia, immunohistochemistry, prostate cancer, TRPC6 PDF | PDF | 中文摘要 | | | | | | | Browse: 3511 | | |