Vitamin D analogues up-regulate p21 and p27 during growth inhibition of pancreatic cancer cell lines (original) (raw)

British Journal of Cancer volume 76, pages 884–889 (1997) Cite this article

Abstract

To obtain information regarding the growth-inhibitory effect of 1,25-dihydroxyvitamin D3 and its non-calcaemic analogue 22-oxa-1,25-dihydroxyvitamin D3 on pancreatic cancer cell lines, differences in the effects of G1-phase cell cycle-regulating factors were studied in vitamin D-responsive and non-responsive cell lines. Levels of expression of cyclins (D1, E and A), cyclin-dependent kinases (2 and 4) and cyclin-dependent kinase inhibitors (p21 and p27) were analysed by Western blotting after treatment with these compounds. In the responsive cells (BxPC-3, Hs 700T and SUP-1), our observations were: (1) marked up-regulation of p21 and p27 after 24 h treatment with 10(-7) mol l(-1) 1,25-dihydroxyvitamin D3 and 22-oxa-1,25-dihydroxyvitamin D3; and (2) marked down-regulation of cyclins, cyclin-dependent kinases and cyclin-dependent kinase inhibitors after 7 days' treatment. In non-responsive cells (Hs 766T and Capan-1), no such changes were observed. In conclusion, vitamin D analogues up-regulate p21 and p27 as an early event, which in turn could block the G1/S transition and induce growth inhibition in responsive cells.

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Authors and Affiliations

  1. The Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan
    S Kawa

Authors

  1. S Kawa
  2. T Nikaido
  3. Y Aoki
  4. Y Zhai
  5. T Kumagai
  6. K Furihata
  7. S Fujii
  8. K Kiyosawa

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Kawa, S., Nikaido, T., Aoki, Y. et al. Vitamin D analogues up-regulate p21 and p27 during growth inhibition of pancreatic cancer cell lines.Br J Cancer 76, 884–889 (1997). https://doi.org/10.1038/bjc.1997.479

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