CTLA4 Silencing with siRNA Promotes Deviation of Th1/Th2 in Chronic Hepatitis B Patients (original) (raw)

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• Published: 01 April 2009

Cellular & Molecular Immunology volume 6, pages 123–127 (2009)Cite this article

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Abstract

To determine whether RNA interference (RNAi) could block cytotoxic T-lymphocyte antigen 4 (CTLA4) in human lymphocytes in vitro and promote IFN-γ and IL-2 secretions, three small interfering RNAs (siRNAs) were selected based on target specificity sequences of human CTLA4 and transfected into human lymphocytes of chronic HBV patients. As a result, the expression of human CTLA4 mRNA was efficiently suppressed by all the three siRNAs. Compared with negative control (siRNA-co), siRNA-1 inhibited the expression of CTLA4 most efficiently and was used in the further study. The expressions of IFN-γ and IL-2 were upregulated and the level of IL-4 was almost unchanged in lymphocytes transfected with siRNA-1 compared with the blank control. These results indicated that siRNA-1 led to IFN-γ and IL-2 secretions, which is a main response of Th1/Th2. In a conclusion, RNAi significantly suppressed the expression of human CTLA4 mRNA in human lymphocytes in vitro, and could induce Th1/Th2 response. It could be a new therapeutic strategy for chronic HBV infection.

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Author notes

1. Yongsheng Yu and Hao Wu: These authors contributed equally to this work

Authors and Affiliations

1. Department of Infectious Diseases, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, 200233, China
   Yongsheng Yu, Hao Wu, Zhenghao Tang & Guoqing Zang

Authors

1. Yongsheng Yu
2. Hao Wu
3. Zhenghao Tang
4. Guoqing Zang

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Correspondence toYongsheng Yu or Guoqing Zang.

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Yu, Y., Wu, H., Tang, Z. et al. CTLA4 Silencing with siRNA Promotes Deviation of Th1/Th2 in Chronic Hepatitis B Patients.Cell Mol Immunol 6, 123–127 (2009). https://doi.org/10.1038/cmi.2009.17

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• Received: 12 January 2008
• Accepted: 30 March 2009
• Issue date: 01 April 2009
• DOI: https://doi.org/10.1038/cmi.2009.17

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