Toll-like receptor 9 dependent activation of MAPK and NF-kB is required for the CpG ODN-induced matrix metalloproteinase-9 expression (original) (raw)

Abstract

Unmethylated CpG oligodeoxynucleotides (CpG ODNs) activate immune cells to produce immune mediators. This study demonstrates that in murine macrophage RAW 264.7 cells, CpG ODN-mediated matrix metalloproteinase-9 (MMP-9) expression is regulated at transcriptional level and requires de novo protein synthesis. Inhibition of ERK and p38 MAPK, but not JNK, results in significant decrease of CpG ODN-induced MMP-9 expression. We found that endosomal maturation inhibitors, chloroquine and bafilomycin A, block CpG ODN-induced ERK and p38 MAPK activation and the subsequent MMP-9 expression. We also observed that CpG ODN induces NF-κ B activation and NF-κ B is a downstream target of p38 MAPK. Taken together, our data demonstrate that CpG ODN triggers MMP-9 expression via TLR-9 dependent ERK and p38 MAPK activation followed by NF-κ B activation.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University, Daegu, 705-717, Korea
    Eun-Jung Lim

Authors

  1. Eun-Jung Lim
  2. Sun-Hye Lee
  3. Jin-Gu Lee
  4. Jae-Ryong Kim
  5. Sung-Su Yun
  6. Suk-Hwan Baek
  7. ChuHee Lee

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