Cohesin relocation from sites of chromosomal loading to places of convergent transcription (original) (raw)
- Letter
- Published: 30 June 2004
- Yuki Katou3,
- Saori Mori4,5,
- Shihori Yokobayashi6,
- Gavin P. Kelly2,
- Takehiko Itoh7,
- Yoshinori Watanabe6,8,
- Katsuhiko Shirahige3,4 na1 &
- …
- Frank Uhlmann1 na1
Nature volume 430, pages 573–578 (2004)Cite this article
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Abstract
Sister chromatids, the products of eukaryotic DNA replication, are held together by the chromosomal cohesin complex after their synthesis. This allows the spindle in mitosis to recognize pairs of replication products for segregation into opposite directions1,2,3,4,5,6. Cohesin forms large protein rings that may bind DNA strands by encircling them7, but the characterization of cohesin binding to chromosomes in vivo has remained vague. We have performed high resolution analysis of cohesin association along budding yeast chromosomes III–VI. Cohesin localizes almost exclusively between genes that are transcribed in converging directions. We find that active transcription positions cohesin at these sites, not the underlying DNA sequence. Cohesin is initially loaded onto chromosomes at separate places, marked by the Scc2/Scc4 cohesin loading complex8, from where it appears to slide to its more permanent locations. But even after sister chromatid cohesion is established, changes in transcription lead to repositioning of cohesin. Thus the sites of cohesin binding and therefore probably sister chromatid cohesion, a key architectural feature of mitotic chromosomes, display surprising flexibility. Cohesin localization to places of convergent transcription is conserved in fission yeast, suggesting that it is a common feature of eukaryotic chromosomes.
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Acknowledgements
We are indebted to E. Schwob for initiating this collaboration. We also thank A. Nakada and T. Chaplin for technical support, R. Rothstein for reagents, and J. Cau, J. Sgouros, J. Svejstrup and members of our laboratories for discussions and comments on the manuscript. A.L. was supported by an EU Marie Curie individual fellowship and a Journal of Cell Science travelling fellowship; K.S. acknowledges support through a MEXT grants-in-aid for priority areas; F.U. was supported by the EMBO Young Investigator programme.
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- Katsuhiko Shirahige and Frank Uhlmann: These authors contributed equally to this work
Authors and Affiliations
- Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, WC2A 3PX, London, UK
Armelle Lengronne & Frank Uhlmann - Computational Genome Analysis Laboratory, Cancer Research UK London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, WC2A 3PX, London, UK
Gavin P. Kelly - Riken Genomic Science Center, Human Genome Research Group, Genome Informatics Team, 1-7-22-W417 Suehiro, Tsurumi, Yokohama City, Kanagawa, 230-0045, Japan
Yuki Katou & Katsuhiko Shirahige - Center for Biological Resources and Informatics, Division of Gene Research, and Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan
Saori Mori & Katsuhiko Shirahige - Science of Biological Supramolecular Systems, Graduate School of Integrated Science, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan
Saori Mori - Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1-1-1, 113-0032, Tokyo, Japan
Shihori Yokobayashi & Yoshinori Watanabe - Research Center for Advanced Science and Technology, Mitsubishi Research Institute Inc., 2-3-6 Ohtemachi, Chiyoda-ku, Tokyo, 100-8141, Japan
Takehiko Itoh - SORST, Japan Science and Technology Agency, Yayoi 1-1-1, Tokyo, 113-0032, Japan
Yoshinori Watanabe
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Lengronne, A., Katou, Y., Mori, S. et al. Cohesin relocation from sites of chromosomal loading to places of convergent transcription.Nature 430, 573–578 (2004). https://doi.org/10.1038/nature02742
- Received: 19 May 2004
- Accepted: 11 June 2004
- Published: 30 June 2004
- Issue Date: 29 July 2004
- DOI: https://doi.org/10.1038/nature02742