Sensitivity to antitubulin chemotherapeutics is regulated by MCL1 and FBW7 (original) (raw)

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Minor errors in the HTML and PDF were corrected on 09March 2011.

Supplementary Fig. S21 contained a duplicated panel instead of the SB203580-treated cdc27 blot.

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Acknowledgements

We thank P. Ekert for FDM cell lines, J. Stinson for sequencing assistance, S. Johnson and C. Santos for assistance with obtaining patient samples, P. Haverty for bioinformatics analysis, C. Grimaldi for cloning assistance, J. Dynek for TaqMan advice, D. French for tumour analysis, C. Quan and J. Tom for peptide synthesis, I. Zilberleyb and the Baculovirus Expression Group for cloning and protein production, S. Charuvu for generating MCL1 point mutants, A. Bruce for graphics assistance, the Genentech Cancer Genome Project Team, Z. Modrusan, R. Soriano and the microarray lab for ovarian tumour data sets, K. Newton for editorial assistance, W. Wei for sharing unpublished results, and A. Eldridge, D. Kirkpatrick, D. Vucic, E. Varfolomeev, T. Goncharov, A. Cochran, O. Huang, A. Huang, Y. Pereg, A. Loktev, D. Phillips, J. Wu, M. van Delft, D. Eaton, E. Shaulian, T. Hunter, S. Cory, J. Adams, A. Strasser, R. Deshaies and G. Evan for discussions. Work in the Huang laboratory is supported by the National Health and Medical Research Council (program grant #461221, IRIISS grant #361646 and a fellowship to D.C.S.H.), the Leukemia and Lymphoma Society (SCOR 7413), the National Institutes of Health (grants CA043540 and CA80188), the Australian Cancer Research Foundation, and an Australian Research Council Australian Postdoctoral fellowship to T.O. We apologize to our colleagues whose primary work could not be cited owing to space constraints.

Author information

Author notes

  1. Cynthia Lam and Toru Okamoto: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Early Discovery Biochemistry, Genentech, South San Francisco, 94080, California, USA
    Ingrid E. Wertz, Saritha Kusam, Cynthia Lam, Elizabeth Helgason, James A. Ernst, Wayne J. Fairbrother & Erin C. Dueber
  2. The Walter and Eliza Hall Institute of Medical Research, Parkville, 3052, Victoria, Australia
    Toru Okamoto & David C. S. Huang
  3. Department of Protein Chemistry, Genentech, South San Francisco, 94080, California, USA
    Wendy Sandoval, James A. Ernst & Jennie R. Lill
  4. Department of Research Oncology, Genentech, South San Francisco, 94080, California, USA
    Daniel J. Anderson, Mike Eby, Lisa D. Belmont, Peter K. Jackson, Mary J. C. Ludlam, Kevin G. Leong & Heather Maecker
  5. Department of Bioinformatics, Genentech, South San Francisco, 94080, California, USA
    Jinfeng Liu & Joshua S. Kaminker
  6. Department of Physiological Chemistry, Genentech, South San Francisco, 94080, California, USA
    Karen M. O’Rourke & Vishva M. Dixit
  7. Department of Molecular Biology, Genentech, South San Francisco, 94080, California, USA
    Kanan Pujara & Somasekar Seshagiri
  8. Department of Biochemical Pharmacology, Genentech, South San Francisco, 94080, California, USA
    Pawan Bir Kohli & Adam R. Johnson
  9. Department of Structural Biology, Abbott Laboratories, Abbott Park, 60064, Illinois, USA
    Mark L. Chiu
  10. Department of Medical Biology, University of Melbourne, Parkville, 3010, Victoria, Australia
    David C. S. Huang

Authors

  1. Ingrid E. Wertz
  2. Saritha Kusam
  3. Cynthia Lam
  4. Toru Okamoto
  5. Wendy Sandoval
  6. Daniel J. Anderson
  7. Elizabeth Helgason
  8. James A. Ernst
  9. Mike Eby
  10. Jinfeng Liu
  11. Lisa D. Belmont
  12. Joshua S. Kaminker
  13. Karen M. O’Rourke
  14. Kanan Pujara
  15. Pawan Bir Kohli
  16. Adam R. Johnson
  17. Mark L. Chiu
  18. Jennie R. Lill
  19. Peter K. Jackson
  20. Wayne J. Fairbrother
  21. Somasekar Seshagiri
  22. Mary J. C. Ludlam
  23. Kevin G. Leong
  24. Erin C. Dueber
  25. Heather Maecker
  26. David C. S. Huang
  27. Vishva M. Dixit

Contributions

I.E.W., S.K., T.O., J.A.E., P.B.K., A.R.J., C.L., E.C.D., E.H., H.M. and K.G.L. designed and performed in vitro, cell-based and in vivo experiments. D.J.A. and M.J.C.L. designed and performed microscopy experiments. S.K., C.L., K.M.O., M.L.C. and M.E. made constructs. J.L. and J.K. performed bioinformatics analysis, K.P. and S.S. provided sequencing analysis. W.S. and J.R.L. designed and performed mass spectrometry experiments. I.E.W., S.K., C.L., T.O., W.S., D.J.A., M.J.C.L., K.G.L., E.C.D., H.M. and V.M.D. prepared the manuscript and figures. W.S., L.D.B., P.K.J., W.J.F., D.J.A., P.B.K., A.R.J., M.J.C.L., H.M., D.C.S.H. and I.E.W. contributed to the study design and data analysis.

Corresponding author

Correspondence toIngrid E. Wertz.

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The authors declare no competing financial interests.

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Supplementary Information

The file contains Supplementary Figures 1-39 with legends, Supplementary Methods, additional references and Supplementary Tables 1-2. (PDF 6785 kb)

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This file contains a corrected version of Supplementary Figure S21 and legend. This file was uploaded on 21 November 2011. (PDF 294 kb)

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Wertz, I., Kusam, S., Lam, C. et al. Sensitivity to antitubulin chemotherapeutics is regulated by MCL1 and FBW7.Nature 471, 110–114 (2011). https://doi.org/10.1038/nature09779

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