SHARPIN forms a linear ubiquitin ligase complex regulating NF-κB activity and apoptosis (original) (raw)

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Acknowledgements

We thank E. Kim, K. Rajalingham and H.-J. Kreienkampfor reagents used in this study, I. Matic for initial MS analysis of HOIP/HOIL-1L samples, S. Wahl for sample preparation, and V. Dötsch and members of the Dikic lab for discussions and comments. This work was supported by grants from the Deutsche Forschungsgemeinschaft (DI 931/3-1), the Cluster of Excellence “Macromolecular Complexes” of the Goethe University Frankfurt (EXC115) to I.D., Landesstiftung Baden-Württemberg to B.M., the Medical Research Council UK to K.R. and B.S., JSPS Postdoctoral Fellowships for Research Abroad to F.I., EMBO long-term fellowship to S.S.S. and The National Institutes of Health (AR049288 to J.P.S.). V.N. was supported by the Unity Through Knowledge Fund, 3B Grant. C.G. acknowledges support from The International Human Frontier Science Program Organization.

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Author notes

1. Yonathan Lissanu Deribe and Sigrid S. Skånland: These authors contributed equally to this work.

Authors and Affiliations

1. Frankfurt Institute for Molecular Life Sciences and Institute of Biochemistry II, Goethe University School of Medicine, Theodor-Stern-Kai 7, D-60590 Frankfurt (Main), Germany,
   Fumiyo Ikeda, Yonathan Lissanu Deribe, Sigrid S. Skånland, Caroline Grabbe, Sjoerd J. L. van Wijk, Panchali Goswami & Ivan Dikic
2. MRC-National Institute for Medical Research, The Ridgeway, London NW7 1AA, UK,
   Benjamin Stieglitz & Katrin Rittinger
3. Department of Molecular Biology, Umeå University, Building 6L, 901 87 Umeå, Sweden,
   Caroline Grabbe
4. Proteome Center Tübingen, Interfaculty Institute for Cell Biology, University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany,
   Mirita Franz-Wachtel & Boris Macek
5. IMBA-Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr. Bohrgasse 3, 1030 Vienna, Austria,
   Vanja Nagy
6. School of Medicine, University of Split, Soltanska 2, Split, HR-21000, Croatia,
   Janos Terzic & Ivan Dikic
7. Department of Biophysics and Biochemistry, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan,
   Fuminori Tokunaga, Tomoko Nakagawa & Kazuhiro Iwai
8. Institute for Genetics, Centre for Molecular Medicine (CMMC), and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Zülpicher Str. 47a, 50674 Cologne, Germany,
   Ariadne Androulidaki & Manolis Pasparakis
9. Cell Biology and Metabolism Group, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan,
   Kazuhiro Iwai
10. The Jackson Laboratory, Bar Harbor, 04609, Maine, USA
    John P. Sundberg
11. Division Nephropharmakologie, Allgemeine Pharmakologie und Toxikologie, Klinikum der Goethe Universität, Theodor-Stern Kai 7, 60590 Frankfurt, Germany,
    Liliana Schaefer

Authors

1. Fumiyo Ikeda
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2. Yonathan Lissanu Deribe
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3. Sigrid S. Skånland
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4. Benjamin Stieglitz
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5. Caroline Grabbe
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6. Mirita Franz-Wachtel
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7. Sjoerd J. L. van Wijk
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8. Panchali Goswami
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9. Vanja Nagy
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10. Janos Terzic
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11. Fuminori Tokunaga
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12. Ariadne Androulidaki
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13. Tomoko Nakagawa
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14. Manolis Pasparakis
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15. Kazuhiro Iwai
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16. John P. Sundberg
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17. Liliana Schaefer
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18. Katrin Rittinger
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19. Boris Macek
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20. Ivan Dikic
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Contributions

F.I., Y.L.D., S.S.S., B.S., C.G., S.J.L.v.W., B.M., V.N., M.F.-W. and P.G. performed the experiments. F.T., A.A. and T.N. contributed with reagents used throughout the study. F.I., Y.L.D., S.S.S., C.G., M.P., J.T., K.I., J.P.S., L.F., B.M. and K.R. contributed to the project by co-ordination of experimental work and writing the manuscript. I.D. provided ideas, co-ordinated the entire project and wrote the manuscript.

Corresponding author

Correspondence toIvan Dikic.

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The authors declare no competing financial interests.

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Ikeda, F., Deribe, Y., Skånland, S. et al. SHARPIN forms a linear ubiquitin ligase complex regulating NF-κB activity and apoptosis.Nature 471, 637–641 (2011). https://doi.org/10.1038/nature09814

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• Received: 06 April 2010
• Accepted: 11 January 2011
• Published: 30 March 2011
• Issue Date: 31 March 2011
• DOI: https://doi.org/10.1038/nature09814