Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease (original) (raw)

• Epstein, S. E. et al. The role of infection in restenosis and atherosclerosis: focus on cytomegalovirus. Lancet 348 (suppl. 1). S13–S17 (1996)
  Article CAS Google Scholar
• Patel, P. et al. Association of Helicobacter pylori and Chlamydia pneumoniae infections with coronary heart disease and cardiovascular risk factors. Br. Med. J. 311, 711–714 (1995)
  Article CAS Google Scholar
• Danesh, J., Collins, R. & Peto, R. Chronic infections and coronary heart disease: is there a link? Lancet 350, 430–436 (1997)
  Article CAS Google Scholar
• Saikku, P. et al. Serological evidence of an association of a novel Chlamydia, TWAR, with chronic coronary heart disease and acute myocardial infarction. Lancet 332, 983–986 (1988)
  Article Google Scholar
• O’Connor, C. M. et al. Azithromycin for the secondary prevention of coronary heart disease events—the WIZARD study: a randomized controlled trial. J. Am. Med. Assoc. 290, 1459–1466 (2003)
  Article Google Scholar
• Cannon, C. P. et al. Antibiotic treatment of Chlamydia pneumoniae after acute coronary syndrome. N. Engl. J. Med. 352, 1646–1654 (2005)
  Article CAS Google Scholar
• Andraws, R., Berger, J. S. & Brown, D. L. Effects of antibiotic therapy on outcomes of patients with coronary artery disease: a meta-analysis of randomized controlled trials. J. Am. Med. Assoc. 293, 2641–2647 (2005)
  Article CAS Google Scholar
• Wright, S. D. et al. Infectious agents are not necessary for murine atherogenesis. J. Exp. Med. 191, 1437–1442 (2000)
  Article CAS Google Scholar
• Backhed, F., Ley, R. E., Sonnenburg, J. L., Peterson, D. A. & Gordon, J. I. Host-bacterial mutualism in the human intestine. Science 307, 1915–1920 (2005)
  Article ADS Google Scholar
• Turnbaugh, P. J. et al. An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 444, 1027–1031 (2006)
  Article ADS Google Scholar
• Dumas, M. E. et al. Metabolic profiling reveals a contribution of gut microbiota to fatty liver phenotype in insulin-resistant mice. Proc. Natl Acad. Sci. USA 103, 12511–12516 (2006)
  Article ADS CAS Google Scholar
• Cashman, J. R. et al. Biochemical and clinical aspects of the human flavin-containing monooxygenase form 3 (FMO3) related to trimethylaminuria. Curr. Drug Metab. 4, 151–170 (2003)
  Article CAS Google Scholar
• Al-Waiz, M., Mikov, M., Mitchell, S. C. & Smith, R. L. The exogenous origin of trimethylamine in the mouse. Metabolism 41, 135–136 (1992)
  Article CAS Google Scholar
• Zeisel, S. H., Mar, M. H., Howe, J. C. & Holden, J. M. Concentrations of choline-containing compounds and betaine in common foods. J. Nutr. 133, 1302–1307 (2003)
  Article CAS Google Scholar
• Lang, D. H. et al. Isoform specificity of trimethylamine _N_-oxygenation by human flavin-containing monooxygenase (FMO) and P450 enzymes: selective catalysis by fmo3. Biochem. Pharmacol. 56, 1005–1012 (1998)
  Article CAS Google Scholar
• Zhang, A. Q., Mitchell, S. C. & Smith, R. L. Dietary precursors of trimethylamine in man: a pilot study. Food Chem. Toxicol. 37, 515–520 (1999)
  Article CAS Google Scholar
• Mitchell, S. C. & Smith, R. L. Trimethylaminuria: the fish malodor syndrome. Drug Metab. Dispos. 29, 517–521 (2001)
  CAS PubMed Google Scholar
• Schadt, E. E. et al. An integrative genomics approach to infer causal associations between gene expression and disease. Nature Genet. 37, 710–717 (2005)
  Article CAS Google Scholar
• Dolphin, C. T., Janmohamed, A., Smith, R. L., Shephard, E. A. & Phillips, I. R. Missense mutation in flavin-containing mono-oxygenase 3 gene, FMO3, underlies fish-odour syndrome. Nature Genet. 17, 491–494 (1997)
  Article CAS Google Scholar
• Wang, S. S. et al. Identification of pathways for atherosclerosis in mice: integration of quantitative trait locus analysis and global gene expression data. Circ. Res. 101, e11–e30 (2007)
  CAS PubMed Google Scholar
• Treberg, J. R., Wilson, C. E., Richards, R. C., Ewart, K. V. & Driedzic, W. R. The freeze-avoidance response of smelt Osmerus mordax: initiation and subsequent suppression of glycerol, trimethylamine oxide and urea accumulation. J. Exp. Biol. 205, 1419–1427 (2002)
  CAS PubMed Google Scholar
• Devlin, G. L., Parfrey, H., Tew, D. J., Lomas, D. A. & Bottomley, S. P. Prevention of polymerization of M and Z α1-Antitrypsin (α1-AT) with trimethylamine _N_-oxide. Implications for the treatment of α1-AT deficiency. Am. J. Respir. Cell Mol. Biol. 24, 727–732 (2001)
  Article CAS Google Scholar
• Song, J. L. & Chuang, D. T. Natural osmolyte trimethylamine _N_-oxide corrects assembly defects of mutant branched-chain α-ketoacid decarboxylase in maple syrup urine disease. J. Biol. Chem. 276, 40241–40246 (2001)
  Article CAS Google Scholar
• Bain, M. A., Faull, R., Fornasini, G., Milne, R. W. & Evans, A. M. Accumulation of trimethylamine and trimethylamine-_N_-oxide in end-stage renal disease patients undergoing haemodialysis. Nephrol. Dial. Transplant. 21, 1300–1304 (2006)
  Article CAS Google Scholar
• Dong, C., Yoon, W. & Goldschmidt-Clermont, P. J. DNA methylation and atherosclerosis. J. Nutr. 132, 2406S–2409S (2002)
  Article CAS Google Scholar
• Zaina, S., Lindholm, M. W. & Lund, G. Nutrition and aberrant DNA methylation patterns in atherosclerosis: more than just hyperhomocysteinemia? J. Nutr. 135, 5–8 (2005)
  Article CAS Google Scholar
• Salmon, W. D. & Newberne, P. M. Cardiovascular disease in choline-deficient rats. Effects of choline deficiency, nature and level of dietary lipids and proteins, and duration of feeding on plasma and liver lipid values and cardiovascular lesions. Arch. Pathol. 73, 190–209 (1962)
  CAS PubMed Google Scholar
• Danne, O., Lueders, C., Storm, C., Frei, U. & Mockel, M. Whole blood choline and plasma choline in acute coronary syndromes: prognostic and pathophysiological implications. Clin. Chim. Acta 383, 103–109 (2007)
  Article CAS Google Scholar
• LeLeiko, R. M. et al. Usefulness of elevations in serum choline and free F2-isoprostane to predict 30-day cardiovascular outcomes in patients with acute coronary syndrome. Am. J. Cardiol. 104, 638–643 (2009)
  Article CAS Google Scholar
• Bidulescu, A., Chambless, L. E., Siega-Riz, A. M., Zeisel, S. H. & Heiss, G. Usual choline and betaine dietary intake and incident coronary heart disease: the Atherosclerosis Risk in Communities (ARIC) study. BMC Cardiovasc. Disord. 7, 20 (2007)
  Article Google Scholar
• Eckburg, P. B. et al. Diversity of the human intestinal microbial flora. Science 308, 1635–1638 (2005)
  Article ADS Google Scholar
• Ley, R. E., Turnbaugh, P. J., Klein, S. & Gordon, J. I. Microbial ecology: human gut microbes associated with obesity. Nature 444, 1022–1023 (2006)
  Article ADS CAS Google Scholar
• Li, M. et al. Symbiotic gut microbes modulate human metabolic phenotypes. Proc. Natl Acad. Sci. USA 105, 2117–2122 (2008)
  Article ADS CAS Google Scholar
• Reigstad, C. S., Lunden, G. O., Felin, J. & Backhed, F. Regulation of serum amyloid A3 (SAA3) in mouse colonic epithelium and adipose tissue by the intestinal microbiota. PLoS ONE 4, e5842 (2009)
  Article ADS Google Scholar
• Martin, F. P. et al. Probiotic modulation of symbiotic gut microbial–host metabolic interactions in a humanized microbiome mouse model. Mol. Syst. Biol. 4, 157 (2008)
  PubMed PubMed Central Google Scholar
• Rizzo, M. L. Statistical Computing with R (Chapman & Hall/CRC, 2008)
  MATH Google Scholar
• Rakoff-Nahoum, S., Paglino, J., Eslami-Varzaneh, F., Edberg, S. & Medzhitov, R. Recognition of commensal microflora by toll-like receptors is required for intestinal homeostasis. Cell 118, 229–241 (2004)
  Article CAS Google Scholar
• Wang, S. et al. Genetic and genomic analysis of a fat mass trait with complex inheritance reveals marked sex specificity. PLoS Genet. 2, e15 (2006)
  Article Google Scholar
• Baglione, J. & Smith, J. D. Quantitative assay for mouse atherosclerosis in the aortic root. Methods Mol. Med. 129, 83–95 (2006)
  CAS PubMed Google Scholar
• Folch, J., Lees, M. & Sloane Stanley, G. H. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226, 497–509 (1957)
  CAS PubMed Google Scholar
• Robinet, P., Wang, Z., Hazen, S. L. & Smith, J. D. A simple and sensitive enzymatic method for cholesterol quantification in macrophages and foam cells. J. Lipid Res. 51, 3364–3369 (2010)
  Article CAS Google Scholar
• Millward, C. A. et al. Genetic factors for resistance to diet-induced obesity and associated metabolic traits on mouse chromosome 17. Mamm. Genome 20, 71–82 (2009)
  Article CAS Google Scholar
• Ahn, S. J., Costa, J. & Emanuel, J. R. PicoGreen quantitation of DNA: effective evaluation of samples pre- or post-PCR. Nucleic Acids Res. 24, 2623–2625 (1996)
  Article CAS Google Scholar
• Wang, Z. et al. Protein carbamylation links inflammation, smoking, uremia and atherogenesis. Nature Med. 13, 1176–1184 (2007)
  Article CAS Google Scholar
• Nicholls, S. J. et al. Lipoprotein (a) levels and long-term cardiovascular risk in the contemporary era of statin therapy. J. Lipid Res. 51, 3055–3061 (2010)
  Article CAS Google Scholar
• Stoves, J., Lindley, E. J., Barnfield, M. C., Burniston, M. T. & Newstead, C. G. MDRD equation estimates of glomerular filtration rate in potential living kidney donors and renal transplant recipients with impaired graft function. Nephrol. Dial. Transplant. 17, 2036–2037 (2002)
  Article Google Scholar
• Barham, A. H. et al. Appropriateness of cholesterol management in primary care by sex and level of cardiovascular risk. Prev. Cardiol. 12, 95–101 (2009)
  Article CAS Google Scholar
• daCosta, K. A., Vrbanac, J. J. & Zeisel, S. H. The measurement of dimethylamine, trimethylamine, and trimethylamine _N_-oxide using capillary gas chromatography-mass spectrometry. Anal. Biochem. 187, 234–239 (1990)
  Article CAS Google Scholar
• Schledzewski, K. et al. Lymphatic endothelium-specific hyaluronan receptor LYVE-1 is expressed by stabilin-1+, F4/80+, CD11b+ macrophages in malignant tumours and wound healing tissue in vivo and in bone marrow cultures in vitro: implications for the assessment of lymphangiogenesis. J. Pathol. 209, 67–77 (2006)
  Article CAS Google Scholar
• Cailhier, J. F. et al. Conditional macrophage ablation demonstrates that resident macrophages initiate acute peritoneal inflammation. J. Immunol. 174, 2336–2342 (2005)
  Article CAS Google Scholar
• Kunjathoor, V. V. et al. Scavenger receptors class A-I/II and CD36 are the principal receptors responsible for the uptake of modified low density lipoprotein leading to lipid loading in macrophages. J. Biol. Chem. 277, 49982–49988 (2002)
  Article CAS Google Scholar
• Yang, X. et al. Tissue-specific expression and regulation of sexually dimorphic genes in mice. Genome Res. 16, 995–1004 (2006)
  Article CAS Google Scholar
• Zhou, J., Lhotak, S., Hilditch, B. A. & Austin, R. C. Activation of the unfolded protein response occurs at all stages of atherosclerotic lesion development in apolipoprotein E-deficient mice. Circulation 111, 1814–1821 (2005)
  Article CAS Google Scholar
• Miles, E. A., Wallace, F. A. & Calder, P. C. Dietary fish oil reduces intercellular adhesion molecule 1 and scavenger receptor expression on murine macrophages. Atherosclerosis 152, 43–50 (2000)
  Article CAS Google Scholar
• Westendorf, T., Graessler, J. & Kopprasch, S. Hypochlorite-oxidized low-density lipoprotein upregulates CD36 and PPARγ mRNA expression and modulates SR-BI gene expression in murine macrophages. Mol. Cell. Biochem. 277, 143–152 (2005)
  Article CAS Google Scholar
• Rasooly, R., Kelley, D. S., Greg, J. & Mackey, B. E. Dietary trans 10, cis 12-conjugated linoleic acid reduces the expression of fatty acid oxidation and drug detoxification enzymes in mouse liver. Br. J. Nutr. 97, 58–66 (2007)
  Article CAS Google Scholar
• Zhang, J. & Cashman, J. R. Quantitative analysis of FMO gene mRNA levels in human tissues. Drug Metab. Dispos. 34, 19–26 (2006)
  Article Google Scholar
• de Vries, T. J., Schoenmaker, T., Hooibrink, B., Leenen, P. J. & Everts, V. Myeloid blasts are the mouse bone marrow cells prone to differentiate into osteoclasts. J. Leukoc. Biol. 85, 919–927 (2009)
  Article CAS Google Scholar
• Chen, F. C. M. & Benoiton, L. N. A new method of quatenizing amines and its use in amino acid and peptide chemistry. Can. J. Chem. 54, 3310–3311 (1976)
  Article CAS Google Scholar
• Morano, C., Zhang, X. & Fricker, L. D. Multiple isotopic labels for quantitative mass spectrometry. Anal. Chem. 80, 9298–9309 (2008)
  Article CAS Google Scholar
• Greenberg, M. E. et al. The lipid whisker model of the structure of oxidized cell membranes. J. Biol. Chem. 283, 2385–2396 (2008)
  Article CAS Google Scholar
• Gauvreau, K. & Pagano, M. Student’s _t_-test. Nutrition 9, 386 (1993)
  CAS PubMed Google Scholar
• Wijnand, H. P. & van de Velde, R. Mann–Whitney/Wilcoxon’s nonparametric cumulative probability distribution. Comput. Methods Programs Biomed. 63, 21–28 (2000)
  Article CAS Google Scholar
• Gaddis, M. L. & Gaddis, G. M. Introduction to biostatistics: part 6, correlation and regression. Ann. Emerg. Med. 19, 1462–1468 (1990)
  Article CAS Google Scholar
• Deichmann, M. et al. S100-β, melanoma-inhibiting activity, and lactate dehydrogenase discriminate progressive from nonprogressive American Joint Committee on Cancer stage IV melanoma. J. Clin. Oncol. 17, 1891–1896 (1999)
  Article CAS Google Scholar
• Goodall, C. M., Stephens, O. B. & Moore, C. M. Comparative sensitivity of survival-adjusted chi-square and normal statistics for the mutagenesis fluctuation assay. J. Appl. Toxicol. 6, 95–100 (1986)
  Article CAS Google Scholar
• Traissac, P., Martin-Prevel, Y., Delpeuch, F. & Maire, B. Logistic regression vs other generalized linear models to estimate prevalence rate ratios. Rev. Epidemiol. Sante Publique 47, 593–604 (1999)
  CAS PubMed Google Scholar
• Gautam, S. Test for linear trend in 2 × K ordered tables with open-ended categories. Biometrics 53, 1163–1169 (1997)
  Article MathSciNet CAS Google Scholar