Senescence surveillance of pre-malignant hepatocytes limits liver cancer development (original) (raw)

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Acknowledgements

We thank D. Largaespada and M. Kay for providing transposon vectors and transposase encoding vectors. We thank L. Gröbe, A. Rinkel, N. Struever, H. Riedesel, the team of the Helmholtz Centre for Infection Research (HZI) animal facility, M. Rothe, K. Schulze, A. Kobold, F. Heinzmann, N. Jedicke, C. Schneider, M. Pesic, H. Klimek and A. Samuels for technical assistance and assistance with animal work, and F. Alves, S. Kimmina, C. Dullin and S. Greco for assistance with flat panel-volumetric computer tomography. We thank the tissue bank of the National Center for Tumor Diseases Heidelberg for providing liver explant tissues. We thank S. Lowe, H. Tillmann, F. Greten and members of the Lowe and Zender laboratory for advice and discussions. This work was supported by the Helmholtz Association of German Research Centres (VH-NG-424 to L.Z.), the German Research Foundation, DFG (Emmy Noether Programme ZE 545/2-1 to L.Z., SFB/TRR77 and the ‘Rebirth’ Cluster of Excellence), the Wilhelm Sander Stiftung, the Bear Necessities Pediatric Cancer Foundation, the Federal German Ministry for Education and Research (BMBF) (ARCHES AWARD to L.Z.) and the European Commission (project ‘Heptromic’). L.Z. holds an adjunct assistant Professorship with the Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA. This work is dedicated to J. Wehland.

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Author notes

  1. Tae-Won Kang and Tetyana Yevsa: These authors contributed equally to this work.

Authors and Affiliations

  1. Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany
    Tae-Won Kang, Tetyana Yevsa, Lisa Hoenicke, Torsten Wuestefeld, Daniel Dauch, Anja Hohmeyer, Marcus Gereke, Ramona Rudalska, Anna Potapova, Siegfried Weiss, Dunja Bruder & Lars Zender
  2. Hannover Medical School, Dept. of Gastroenterology, Hepatology and Endocrinology, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany
    Tetyana Yevsa, Norman Woller, Torsten Wuestefeld, Anja Hohmeyer, Michael Manns & Lars Zender
  3. Twincore Centre for Experimental and Clinical Infection Research, Feodor-Lynen Strasse 7, 30625 Hannover, Germany
    Marcus Iken & Michael Ott
  4. Department of Internal Medicine III, University Hospital Aachen, 52074 Aachen, Germany
    Mihael Vucur, Frank Tacke & Tom Luedde
  5. Department of Pathology, University Hospital Zurich, CH 8091 Zurich, Switzerland
    Mathias Heikenwalder
  6. Institute of Virology, Helmholtz Centre Munich, Technical University Munich, 81675 Munich, Germany
    Mathias Heikenwalder
  7. MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College, Hammersmith Campus, London W12 0NN, UK
    Sadaf Khan & Jesus Gil
  8. University of Heidelberg, Institute of Pathology, Im Neuenheimer Feld 220/21, 69120 Heidelberg, Germany
    Peter Schirmacher & Thomas Longerich
  9. Clinic for Internal Medicine, District Hospital Reutlingen, Steinenbergstrasse 31, 72764 Reutlingen, Germany
    Stefan Kubicka

Authors

  1. Tae-Won Kang
  2. Tetyana Yevsa
  3. Norman Woller
  4. Lisa Hoenicke
  5. Torsten Wuestefeld
  6. Daniel Dauch
  7. Anja Hohmeyer
  8. Marcus Gereke
  9. Ramona Rudalska
  10. Anna Potapova
  11. Marcus Iken
  12. Mihael Vucur
  13. Siegfried Weiss
  14. Mathias Heikenwalder
  15. Sadaf Khan
  16. Jesus Gil
  17. Dunja Bruder
  18. Michael Manns
  19. Peter Schirmacher
  20. Frank Tacke
  21. Michael Ott
  22. Tom Luedde
  23. Thomas Longerich
  24. Stefan Kubicka
  25. Lars Zender

Contributions

L.Z. designed and guided the research and wrote the manuscript. T.-W.K., T.Y. and N.W. conducted experiments and contributed to research design and manuscript preparation. L.H., T.W., D.D., A.H., M.G., R.R., A.P., M.I., M.V., S.W., M.H., S.K., J.G., F.T., To.L., D.B. M.M., M.O. and S.K. contributed to research design and/or conducted experiments. P.S. and Th.L. performed histopathological analyses.

Corresponding author

Correspondence toLars Zender.

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Competing interests

The authors declare no competing financial interests.

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Kang, TW., Yevsa, T., Woller, N. et al. Senescence surveillance of pre-malignant hepatocytes limits liver cancer development.Nature 479, 547–551 (2011). https://doi.org/10.1038/nature10599

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