Tissue factor and PAR1 promote microbiota-induced intestinal vascular remodelling (original) (raw)

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Acknowledgements

We thank R. Perkins for editing the manuscript; C. Arvidsson, A. Hallén, S. Wagoner, M. Karlsson, D. O’Donell, S. Islam, N. Hörmann and A. Mohammadzadeh for technical assistance; A. Hallén for providing Supplementary Fig. 16; and P. Lindahl, J. Gordon, C. Betsholtz, M. Bergö, A. Wichmann, V. Tremaroli, M. Levin and S. Massberg for comments and suggestions. We are grateful to D. Kirchhofer for the gift of 1H1 monoclonal anti-mouse TF antibody, J. Nichols at Amgen for mL4-3, N. Mackman for the low-TF mice, M. Anderson for the human TF knock-in mice, and J. Gordon for providing CR2-_tox_176 mice. This study was supported by the Swedish Foundation for Strategic Research, the Swedish Research Council, Torsten and Ragnar Söderberg’s foundation, Petrus and Augusta Hedlund’s foundation, and the Swedish federal government under the LUA/ALF agreement to F.B., National Institutes of Health grants HL-60742 and HL-77753 to W.R., and a Marie Curie Fellowship, a Marie Curie Reintegration Grant from the European Union and the German Federal Ministry of Education and Research (BMBF 01EO1003) to C.R.

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Authors and Affiliations

  1. Sahlgrenska Center for Cardiovascular and Metabolic Research/Wallenberg Laboratory, University of Gothenburg, 413 45 Gothenburg, Sweden,
    Christoph Reinhardt, Mattias Bergentall, Thomas U. Greiner, Gunnel Östergren-Lundén & Fredrik Bäckhed
  2. Department of Molecular and Clinical Medicine, University of Gothenburg, 413 45 Gothenburg, Sweden,
    Christoph Reinhardt, Mattias Bergentall, Thomas U. Greiner, Gunnel Östergren-Lundén & Fredrik Bäckhed
  3. Center for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Experimental Research, 55131 Mainz, Germany,
    Christoph Reinhardt
  4. Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, 92037, California, USA
    Florence Schaffner & Wolfram Ruf
  5. Haemostasis Biology, Novo Nordisk A/S, DK-2760 Maalov, Denmark,
    Lars C. Petersen
  6. Novo Nordisk Foundation Center for Basic Metabolic Research, Section for Metabolic Receptology and Enteroendocrinology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, DK-2200, Denmark
    Fredrik Bäckhed

Authors

  1. Christoph Reinhardt
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  2. Mattias Bergentall
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  3. Thomas U. Greiner
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  4. Florence Schaffner
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  5. Gunnel Östergren-Lundén
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  6. Lars C. Petersen
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  7. Wolfram Ruf
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  8. Fredrik Bäckhed
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Contributions

C.R. was responsible for conception and study design, biochemical analysis of TF, analysis of vessel densities, data assembly and analysis, and writing the manuscript. M.B., T.U.G., F.S. and G.Ö.L. performed data collection, analysis and interpretation and commented on the manuscript. L.C.P. provided material. W.R. and F.B. were responsible for conception and study design, data analysis and interpretation, and writing the manuscript.

Corresponding author

Correspondence toFredrik Bäckhed.

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Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Information

This file contains Supplementary Figures 1-17 and Supplementary Table 1. (PDF 2185 kb)

Supplementary Movie 1

This movie shows a three dimensional rendering of a small intestinal epithelial cell stained for tissue factor (red), cytokeratin (green), and nuclei (blue). (MOV 5 kb)

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Reinhardt, C., Bergentall, M., Greiner, T. et al. Tissue factor and PAR1 promote microbiota-induced intestinal vascular remodelling.Nature 483, 627–631 (2012). https://doi.org/10.1038/nature10893

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