A new antibiotic kills pathogens without detectable resistance (original) (raw)

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Primary accessions

GenBank/EMBL/DDBJ

Data deposits

The biosynthetic gene cluster for teixobactin has been deposited with GenBank under accession number KP006601.

Change history

Two minor typos were corrected in the main text and Methods.

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Acknowledgements

This work was supported by NIH grant T-RO1 AI085585 to K.L., by NIH grant AI085612 to A.L.S., by the Charles A. King Trust to B.P.C., and by the German Research Foundation (DFG; SCHN1284/1-2) and the German Center for Infection Research (DZIF) to T.S. and I.E. The NRS strains were provided by the Network on Antimicrobial Resistance in Staphylococcus aureus for distribution by BEI Resources, NIAID, NIH. Preclinical Services offered by NIAID are gratefully acknowledged. We thank H. G. Sahl for reading the manuscript and making comments, A. Makriyannis for suggestions, P. Muller, B. Berdy and S. Kaluziak for taxonomy analysis, and M. Josten for performing mass spectrometry analysis.

Author information

Author notes

  1. Losee L. Ling and Tanja Schneider: These authors contributed equally to this work.

Authors and Affiliations

  1. NovoBiotic Pharmaceuticals, Cambridge, 02138, Massachusetts, USA
    Losee L. Ling, Aaron J. Peoples, Amy L. Spoering, Dallas E. Hughes, Douglas R. Cohen, Cintia R. Felix, K. Ashley Fetterman, William P. Millett, Anthony G. Nitti & Ashley M. Zullo
  2. Institute of Medical Microbiology, Immunology and Parasitology—Pharmaceutical Microbiology Section, University of Bonn, Bonn 53115, Germany,
    Tanja Schneider, Ina Engels & Anna Mueller
  3. German Centre for Infection Research (DZIF), Partner Site Bonn-Cologne, 53115 Bonn, Germany,
    Tanja Schneider, Ina Engels, Anna Mueller & Till F. Schäberle
  4. Department of Biology, Antimicrobial Discovery Center, Northeastern University, Boston, 02115, Massachusetts, USA
    Brian P. Conlon, Chao Chen & Kim Lewis
  5. Institute for Pharmaceutical Biology, University of Bonn, Bonn 53115, Germany,
    Till F. Schäberle
  6. Department of Biology, Northeastern University, Boston, 02115, Massachusetts, USA
    Slava Epstein
  7. Selcia, Ongar, Essex CM5 0GS, UK,
    Michael Jones, Linos Lazarides & Victoria A. Steadman

Authors

  1. Losee L. Ling
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  2. Tanja Schneider
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  3. Aaron J. Peoples
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  4. Amy L. Spoering
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  5. Ina Engels
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  6. Brian P. Conlon
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  7. Anna Mueller
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  8. Till F. Schäberle
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  9. Dallas E. Hughes
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  10. Slava Epstein
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  11. Michael Jones
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  12. Linos Lazarides
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  13. Victoria A. Steadman
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  14. Douglas R. Cohen
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  15. Cintia R. Felix
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  16. K. Ashley Fetterman
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  17. William P. Millett
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  18. Anthony G. Nitti
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  19. Ashley M. Zullo
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  20. Chao Chen
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  21. Kim Lewis
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Contributions

K.L. and T.S. designed the study, analysed results, and wrote the paper. L.L.L. designed the study and analysed results. A.J.P. designed the study, performed compound isolation and structure determination and analysed data. B.P.C. designed the study, performed susceptibility experiments and wrote the paper. D.E.H. oversaw preclinical work including designing studies and analysing data. S.E. designed cultivation experiments and analysed data. M.J., L.L. and V.A.S. designed and performed experiments on structure determination and analysed data. I.E. and A.M. designed and performed experiments on mechanism of action. A.L.S., D.R.C., C.R.F., K.A.F., W.P.M., A.G.N., A.M.Z. and C.C. performed experiments on compound production, isolation, susceptibility testing and data analysis. T.F.S. identified the biosynthetic cluster.

Corresponding author

Correspondence toKim Lewis.

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Competing interests

The following authors, L. L. Ling, A. J. Peoples, A. L. Spoering, D. E. Hughes, D. R. Cohen, C. R. Felix, K. A. Fetterman, W. P. Millett, A. G. Nitti, A. M. Zullo, K. Lewis, and S. Epstein, declare competing financial interests as they are employees and consultants of NovoBiotic Pharmaceuticals.

Extended data figures and tables

Extended Data Figure 1 The iChip.

a–c, The iChip (a) consists of a central plate (b) which houses growing microorganisms, semi-permeable membranes on each side of the plate, which separate the plate from the environment, and two supporting side panels (c). The central plate and side panels have multiple matching through-holes. When the central plate is dipped into suspension of cells in molten agar, the through-holes capture small volumes of this suspension, which solidify in the form of small agar plugs. Alternatively, molten agar can be dispensed into the chambers. The membranes are attached and the iChip is then placed in soil from which the sample originated.

Extended Data Figure 2 16S rRNA gene phylogeny of Eleftheria terrae.

a, The phylogenetic position of E. terrae within the class β-proteobacteria. The 16S rRNA gene sequences were downloaded from Entrez at NCBI using accession numbers retrieved from peer-reviewed publications. b, The phylogenetic position of E. terrae among its closest known relatives. The sequences were downloaded from NCBI using accession numbers retrieved from the RDP Classifier Database. For both trees, multiple sequence alignments (MSA) were constructed using ClustalW2, implementing a default Cost Matrix, the Neighbour-Joining (NJ) clustering algorithm, as well as optimized gap penalties. Resulting alignments were manually curated and phylogenetic trees were constructed leveraging PhyML 3.0 with a TN93 substitution model and 500 Bootstrap iterations of branch support. Topology search optimization was conducted using the Subtree–Pruning–Regrafting (SPR) algorithm with an estimated Transition–Transversion ratio and gamma distribution parameters as well as fixed proportions of invariable sites.

Extended Data Figure 3 NMR assignment of teixobactin.

a, 13C-NMR of teixobactin (125 mHz, δ in p.p.m.). b, Structure of teixobactin with the NMR assignments.

Extended Data Figure 4 NMR spectra of teixobactin.

a, 13C NMR spectrum of teixobactin. b, 1H NMR spectrum. c, HMBC NMR spectrum. d, HSQC NMR spectrum. e, COSY NMR spectrum.

Extended Data Figure 5 Hypothetical biosynthesis pathway of teixobactin.

The eleven modules of the non-ribosomal peptide synthetases Txo1 and Txo2 are depicted with the growing chain attached. Each module is responsible for the incorporation of one specific amino acid in the nascent peptide chain. The _N_-methylation of the first amino acid phenylalanine is catalysed by the methyltransferase domain in module 1. The ring closure (marked by a dashed arrow) between the last isoleucine and threonine is catalysed by the thioesterase domains during molecule off-loading, resulting in teixobactin.

Extended Data Figure 6 Teixobactin activity against vancomycin-resistant strains.

a, Vancomycin intermediate S. aureus (VISA) were grown to late exponential phase and challenged with vancomycin or teixobactin. Cell numbers were determined by plating for colony counts. Data are representative of 3 independent experiments ± s.d. b, Complex formation of teixobactin with cell wall precursor variants as formed by vancomycin-resistant strains. Purified lipid intermediates with altered stem peptides were incubated with teixobactin at a molar ratio of 2:1 (TEIX:lipid II variant). Reaction mixtures were extracted with BuOH/PyrAc and binding of teixobactin to lipid II variants is indicated by its absence on the thin-layer chromatogram. Migration behaviour of unmodified lipid II is used for comparison. The figure is representative of 3 independent experiments.

Extended Data Figure 7 Model for the mechanism of action of teixobactin.

Inhibition of cell wall synthesis by teixobactin. Lipid II, precursor of peptidoglycan, is synthesized in the cytoplasm and flipped to the surface of the inner membrane by MurJ48 or FtsW49. Lipid III, a precursor of wall teichoic acid (WTA), is similarly formed inside the cell and WTA lipid-bound precursors are translocated across the cytoplasmic membrane by the ABC-transporter TarGH50. Teixobactin (TEIX) forms a stoichiometric complex with cell wall precursors, lipid II and lipid III. Abduction of these building blocks simultaneously interrupts peptidoglycan (right), WTA (left) biosynthesis as well as precursor recycling. Binding to multiple targets within the cell wall pathways obstructs the formation of a functional cell envelope. Left panel, teixobactin targeting and resistance. The producer of teixobactin is a Gram-negative bacterium which is protected from this compound by exporting it outside of its outer membrane permeability barrier. The target Gram-positive organisms do not have an outer membrane. CM, cytoplasmic membrane; CW, cell wall; OM, outer membrane; LTA, lipoteichoic acid; WTA, wall teichoic acid.

Extended Data Figure 8 Pharmacokinetic analysis of teixobactin.

a, The mean plasma concentrations of teixobactin after a single i.v. injection of 20 mg per kg teixobactin (3 mice per time point). Data are the mean of plasma concentration, and error bars represent the standard deviation from 3 animals in each time point. b, Pharmacokinetic parameters of teixobactin calculated with a non-compartmental analysis model based on WinNonlin.

Extended Data Table 1 Antibacterial spectrum of teixobactin

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Extended Data Table 2 Antagonization of the antimicrobial activity of teixobactin by cell wall precursors

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Ling, L., Schneider, T., Peoples, A. et al. A new antibiotic kills pathogens without detectable resistance.Nature 517, 455–459 (2015). https://doi.org/10.1038/nature14098

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