A functionally characterized test set of human induced pluripotent stem cells (original) (raw)

Change history

In the version of this article initially published online, a funder was inadvertently omitted from the Acknowledgments. The error has been corrected for the print, PDF and HTML versions of this article.

References

  1. Kiskinis, E. & Eggan, K. Progress toward the clinical application of patient-specific pluripotent stem cells. J. Clin. Invest. 120, 51–59 (2010).
    Article CAS Google Scholar
  2. Dimos, J.T. et al. Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons. Science 321, 1218–1221 (2008).
    Article CAS Google Scholar
  3. Park, I.H. et al. Disease-specific induced pluripotent stem cells. Cell 134, 877–886 (2008).
    Article CAS Google Scholar
  4. Lee, G. et al. Modelling pathogenesis and treatment of familial dysautonomia using patient-specific iPSCs. Nature 461, 402–406 (2009).
    Article CAS Google Scholar
  5. Soldner, F. et al. Parkinson's disease patient-derived induced pluripotent stem cells free of viral reprogramming factors. Cell 136, 964–977 (2009).
    Article CAS Google Scholar
  6. Chin, M.H. et al. Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures. Cell Stem Cell 5, 111–123 (2009).
    Article CAS Google Scholar
  7. Doi, A. et al. Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts. Nat. Genet. 41, 1350–1353 (2009).
    Article CAS Google Scholar
  8. Stadtfeld, M. et al. Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells. Nature 465, 175–181 (2010).
    Article CAS Google Scholar
  9. Hu, B.Y. et al. Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency. Proc. Natl. Acad. Sci. USA 107, 4335–4340 (2010).
    Article CAS Google Scholar
  10. Kanning, K.C., Kaplan, A. & Henderson, C.E. Motor neuron diversity in development and disease. Annu. Rev. Neurosci. 33, 409–440 (2010).
    Article CAS Google Scholar
  11. Bock, C. et al. Reference maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines. Cell published online, doi:10.1016/j.cell.2010.12.032 (3 February 2011).
  12. Di Giorgio, F.P., Boulting, G.L., Bobrowicz, S. & Eggan, K.C. Human embryonic stem cell-derived motor neurons are sensitive to the toxic effect of glial cells carrying an ALS-causing mutation. Cell Stem Cell 3, 637–648 (2008).
    Article CAS Google Scholar
  13. Wichterle, H., Lieberam, I., Porter, J.A. & Jessell, T.M. Directed differentiation of embryonic stem cells into motor neurons. Cell 110, 385–397 (2002).
    Article CAS Google Scholar
  14. Arber, S. et al. Requirement for the homeobox gene Hb9 in the consolidation of motor neuron identity. Neuron 23, 659–674 (1999).
    Article CAS Google Scholar
  15. Chambers, S.M. et al. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat. Biotechnol. 27, 275–280 (2009).
    Article CAS Google Scholar
  16. Zhou, J. et al. High-efficiency induction of neural conversion in human ESCs and human induced pluripotent stem cells with a single chemical inhibitor of transforming growth factor beta superfamily receptors. Stem Cells 28, 1741–1750 (2010).
    Article CAS Google Scholar
  17. Taura, D. et al. Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells. FEBS Lett. 583, 1029–1033 (2009).
    Article CAS Google Scholar
  18. Tokumoto, Y., Ogawa, S., Nagamune, T. & Miyake, J. Comparison of efficiency of terminal differentiation of oligodendrocytes from induced pluripotent stem cells versus embryonic stem cells in vitro. J. Biosci. Bioeng. 109, 622–628 (2010).
    Article CAS Google Scholar
  19. Xi, J. et al. Comparison of contractile behavior of native murine ventricular tissue and cardiomyocytes derived from embryonic or induced pluripotent stem cells. FASEB J. 24, 2739–2751 (2010).
    Article CAS Google Scholar
  20. Armstrong, L. et al. Human induced pluripotent stem cell lines show stress defense mechanisms and mitochondrial regulation similar to those of human embryonic stem cells. Stem Cells 28, 661–673 (2010).
    Article CAS Google Scholar
  21. Ghosh, Z. et al. Persistent donor cell gene expression among human induced pluripotent stem cells contributes to differences with human embryonic stem cells. PLoS ONE 5, e8975 (2010).
    Article Google Scholar
  22. Grigoriadis, A.E. et al. Directed differentiation of hematopoietic precursors and functional osteoclasts from human ES and iPS cells. Blood 115, 2769–2776 (2010).
    Article CAS Google Scholar
  23. Cowan, C.A. et al. Derivation of embryonic stem-cell lines from human blastocysts. N. Engl. J. Med. 350, 1353–1356 (2004).
    Article CAS Google Scholar
  24. James, D., Noggle, S.A., Swigut, T. & Brivanlou, A.H. Contribution of human embryonic stem cells to mouse blastocysts. Dev. Biol. 295, 90–102 (2006).
    Article CAS Google Scholar

Download references

Acknowledgements

We thank H. Mitsumoto, J. Montes, P. Kaufmann and J. Andrews for collecting skin biopsies; K. Koszka, A. Sproul, A. Hon and A. Garcia-Diaz for technical assistance; M. Park, A. Meissner and C. Bock for manuscript assistance, as well as S. Brenner-Morton and T. Jessell for providing Islet antibodies. This work was funded by Project A.L.S., P2ALS, NYSTEM and the National Institutes of Health (NIH) GO grant 1RC2 NS069395-01. G.L.B. is a Harvard Stem Cell Institute/NIH Trainee. E.K. is an EMBO Postdoctoral Fellow. B.J.W. is supported by NIH Training Grant 5T32GM007592. C.J.W. is supported by grants from the National Institute of Neurological Disorders and Stroke and the National Institute of Child Health and Development. K.E. is a Howard Hughes Medical Institute early career scientist.

Author information

Author notes

  1. John T Dimos
    Present address: Present address: iPierian, Inc., South San Francisco, California, USA.,
  2. Gabriella L Boulting, Evangelos Kiskinis, Gist F Croft, Mackenzie W Amoroso and Derek H Oakley: These authors contributed equally to this work.

Authors and Affiliations

  1. The Howard Hughes Medical Institute, Cambridge, Massachusetts, USA
    Gabriella L Boulting, Evangelos Kiskinis, Mariko Yamaki & Kevin Eggan
  2. Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, USA
    Gabriella L Boulting, Evangelos Kiskinis, Mariko Yamaki, Lance Davidow, Shravani Mikkilineni & Kevin Eggan
  3. Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA
    Gabriella L Boulting, Christopher T Rodolfa, John T Dimos, Shravani Mikkilineni & Kevin Eggan
  4. Project A.L.S./Jenifer Estess Laboratory for Stem Cell Research, Columbia University, New York, New York, USA
    Gist F Croft, Mackenzie W Amoroso, Derek H Oakley, Christopher E Henderson & Hynek Wichterle
  5. Departments of Pathology, Neurology and Neuroscience, Columbia University, Center for Motor Neuron Biology and Disease (MNC), and Columbia Stem Cell Initiative (CSCI), New York, New York, USA
    Gist F Croft, Mackenzie W Amoroso, Derek H Oakley, Christopher E Henderson & Hynek Wichterle
  6. Program in Neurobiology and FM Kirby Neurobiology Center, Children's Hospital Boston, Boston, Massachusetts, USA
    Brian J Wainger & Clifford J Woolf
  7. Department of Neurobiology, Harvard Medical School, Boston, Massachusetts, USA
    Brian J Wainger & Clifford J Woolf
  8. Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA
    Brian J Wainger
  9. Departments of Physiology and Cellular Biophysics, and Neuroscience, Columbia University, New York, New York, USA
    Damian J Williams & Amy B MacDermott
  10. The New York Stem Cell Foundation, Inc. (NYSCF), New York, New York, USA
    David J Kahler

Authors

  1. Gabriella L Boulting
    You can also search for this author inPubMed Google Scholar
  2. Evangelos Kiskinis
    You can also search for this author inPubMed Google Scholar
  3. Gist F Croft
    You can also search for this author inPubMed Google Scholar
  4. Mackenzie W Amoroso
    You can also search for this author inPubMed Google Scholar
  5. Derek H Oakley
    You can also search for this author inPubMed Google Scholar
  6. Brian J Wainger
    You can also search for this author inPubMed Google Scholar
  7. Damian J Williams
    You can also search for this author inPubMed Google Scholar
  8. David J Kahler
    You can also search for this author inPubMed Google Scholar
  9. Mariko Yamaki
    You can also search for this author inPubMed Google Scholar
  10. Lance Davidow
    You can also search for this author inPubMed Google Scholar
  11. Christopher T Rodolfa
    You can also search for this author inPubMed Google Scholar
  12. John T Dimos
    You can also search for this author inPubMed Google Scholar
  13. Shravani Mikkilineni
    You can also search for this author inPubMed Google Scholar
  14. Amy B MacDermott
    You can also search for this author inPubMed Google Scholar
  15. Clifford J Woolf
    You can also search for this author inPubMed Google Scholar
  16. Christopher E Henderson
    You can also search for this author inPubMed Google Scholar
  17. Hynek Wichterle
    You can also search for this author inPubMed Google Scholar
  18. Kevin Eggan
    You can also search for this author inPubMed Google Scholar

Contributions

G.F.C., M.W.A. and D.H.O. maintained human fibroblasts. C.T.R. and J.T.D. reprogrammed all iPSC lines. G.L.B. and E.K. expanded all iPSC lines. G.L.B. and E.K. led and contributed equally to all other experiments and analyses in the Eggan laboratory. G.F.C., M.W.A. and D.H.O. led and contributed equally to all other experiments and analyses in the Project ALS laboratory. D.J.K. did FC analysis. A.B.M., D.J.W. and D.H.O. designed and carried out Ca2+ imaging. B.J.W., G.L.B. and C.J.W. did recordings. M.Y. assisted with teratomas. L.D. assisted with quantitative analysis. S.M. assisted with stem cell culture. G.L.B., E.K., K.E., G.F.C., M.W.A., D.H.O., C.E.H. and H.W. conceived the experiments and wrote the manuscript.

Corresponding authors

Correspondence toHynek Wichterle or Kevin Eggan.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Rights and permissions

About this article

Cite this article

Boulting, G., Kiskinis, E., Croft, G. et al. A functionally characterized test set of human induced pluripotent stem cells.Nat Biotechnol 29, 279–286 (2011). https://doi.org/10.1038/nbt.1783

Download citation