A functionally characterized test set of human induced pluripotent stem cells (original) (raw)
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11 February 2011
In the version of this article initially published online, a funder was inadvertently omitted from the Acknowledgments. The error has been corrected for the print, PDF and HTML versions of this article.
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Acknowledgements
We thank H. Mitsumoto, J. Montes, P. Kaufmann and J. Andrews for collecting skin biopsies; K. Koszka, A. Sproul, A. Hon and A. Garcia-Diaz for technical assistance; M. Park, A. Meissner and C. Bock for manuscript assistance, as well as S. Brenner-Morton and T. Jessell for providing Islet antibodies. This work was funded by Project A.L.S., P2ALS, NYSTEM and the National Institutes of Health (NIH) GO grant 1RC2 NS069395-01. G.L.B. is a Harvard Stem Cell Institute/NIH Trainee. E.K. is an EMBO Postdoctoral Fellow. B.J.W. is supported by NIH Training Grant 5T32GM007592. C.J.W. is supported by grants from the National Institute of Neurological Disorders and Stroke and the National Institute of Child Health and Development. K.E. is a Howard Hughes Medical Institute early career scientist.
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Author notes
- John T Dimos
Present address: Present address: iPierian, Inc., South San Francisco, California, USA., - Gabriella L Boulting, Evangelos Kiskinis, Gist F Croft, Mackenzie W Amoroso and Derek H Oakley: These authors contributed equally to this work.
Authors and Affiliations
- The Howard Hughes Medical Institute, Cambridge, Massachusetts, USA
Gabriella L Boulting, Evangelos Kiskinis, Mariko Yamaki & Kevin Eggan - Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, USA
Gabriella L Boulting, Evangelos Kiskinis, Mariko Yamaki, Lance Davidow, Shravani Mikkilineni & Kevin Eggan - Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA
Gabriella L Boulting, Christopher T Rodolfa, John T Dimos, Shravani Mikkilineni & Kevin Eggan - Project A.L.S./Jenifer Estess Laboratory for Stem Cell Research, Columbia University, New York, New York, USA
Gist F Croft, Mackenzie W Amoroso, Derek H Oakley, Christopher E Henderson & Hynek Wichterle - Departments of Pathology, Neurology and Neuroscience, Columbia University, Center for Motor Neuron Biology and Disease (MNC), and Columbia Stem Cell Initiative (CSCI), New York, New York, USA
Gist F Croft, Mackenzie W Amoroso, Derek H Oakley, Christopher E Henderson & Hynek Wichterle - Program in Neurobiology and FM Kirby Neurobiology Center, Children's Hospital Boston, Boston, Massachusetts, USA
Brian J Wainger & Clifford J Woolf - Department of Neurobiology, Harvard Medical School, Boston, Massachusetts, USA
Brian J Wainger & Clifford J Woolf - Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA
Brian J Wainger - Departments of Physiology and Cellular Biophysics, and Neuroscience, Columbia University, New York, New York, USA
Damian J Williams & Amy B MacDermott - The New York Stem Cell Foundation, Inc. (NYSCF), New York, New York, USA
David J Kahler
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- Gabriella L Boulting
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Contributions
G.F.C., M.W.A. and D.H.O. maintained human fibroblasts. C.T.R. and J.T.D. reprogrammed all iPSC lines. G.L.B. and E.K. expanded all iPSC lines. G.L.B. and E.K. led and contributed equally to all other experiments and analyses in the Eggan laboratory. G.F.C., M.W.A. and D.H.O. led and contributed equally to all other experiments and analyses in the Project ALS laboratory. D.J.K. did FC analysis. A.B.M., D.J.W. and D.H.O. designed and carried out Ca2+ imaging. B.J.W., G.L.B. and C.J.W. did recordings. M.Y. assisted with teratomas. L.D. assisted with quantitative analysis. S.M. assisted with stem cell culture. G.L.B., E.K., K.E., G.F.C., M.W.A., D.H.O., C.E.H. and H.W. conceived the experiments and wrote the manuscript.
Corresponding authors
Correspondence toHynek Wichterle or Kevin Eggan.
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The authors declare no competing financial interests.
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Boulting, G., Kiskinis, E., Croft, G. et al. A functionally characterized test set of human induced pluripotent stem cells.Nat Biotechnol 29, 279–286 (2011). https://doi.org/10.1038/nbt.1783
- Received: 28 October 2010
- Accepted: 19 January 2011
- Published: 03 February 2011
- Issue Date: March 2011
- DOI: https://doi.org/10.1038/nbt.1783