Continuous high-titer HIV-1 vector production (original) (raw)

References

  1. Sparacio, S., Pfeiffer, T., Schaal, H. & Bosch, V. Generation of a flexible cell line with regulatable, high-level expression of HIV Gag/Pol particles capable of packaging HIV-derived vectors. Mol. Ther. 3, 602–612 (2001).
    Article CAS PubMed Google Scholar
  2. Kaul, M., Yu, H., Ron, Y. & Dougherty, J.P. Regulated lentiviral packaging cell line devoid of most viral _cis_-acting sequences. Virology 249, 167–174 (2001).
    Article Google Scholar
  3. Klages, N., Zufferey, R. & Trono, D. A stable system for the high-titer production of multiply attenuated lentiviral vectors. Mol. Ther. 2, 170–176 (2000).
    Article CAS PubMed Google Scholar
  4. Srinivasakumar, N. et al. The effect of viral regulatory protein expression on gene delivery by human immunodeficiency virus type 1 vectors produced in stable packaging cell lines. J. Virol. 71, 5841–5848 (1997).
    CAS PubMed PubMed Central Google Scholar
  5. Corbeau, P., Kraus, G. & Wong-Staal, F. Efficient gene transfer by a human immunodeficiency virus type 1 (HIV-1)-derived vector utilizing a stable HIV packaging cell line. Proc. Natl. Acad. Sci. USA 93, 14070–14075 (1996).
    Article CAS PubMed PubMed Central Google Scholar
  6. Carroll, R. et al. A human immunodeficiency virus type 1 (HIV-1)-based retroviral vector system utilizing stable HIV-1 packaging cell lines. J. Virol. 68, 6047–6051 (1994).
    CAS PubMed PubMed Central Google Scholar
  7. Kaplan, A.H. & Swanstrom, R. Human immunodeficiency virus type 1 Gag proteins are processed in two cellular compartments. Proc. Natl. Acad. Sci. USA 88, 4528–4532 (1991).
    Article CAS PubMed PubMed Central Google Scholar
  8. Hoxie, J.A., Haggarty, B.S., Rackowski, J.L., Pillsbury, N. & Levy, J.A. Persistent noncytopathic infection of normal human T lymphocytes with AIDS-associated retrovirus. Science 229, 1400–1402 (1985).
    Article CAS PubMed Google Scholar
  9. Ott, D.E., Nigida, S.M., Henderson, J.L.E. & Arthur, L.O. The majority of cells are superinfected in a cloned cell line that produces high levels of human immunodeficiency virus type 1 strain MN. J. Virol. 69, 2443–2450 (1995).
    CAS PubMed PubMed Central Google Scholar
  10. Kotsopoulo, E., Kim, V.N., Kingsman, A.J., Kingsman, S.M. & Mitrophanous, K.A. A Rev-independent human immunodeficiency virus type 1 (HIV-1)-based vector that exploits a codon-optimized HIV gag-pol gene. J. Virol. 74, 4839–4852 (2000).
    Article Google Scholar
  11. Soneoka, Y. et al. A transient three-plasmid expression system for the production of high-titer retroviral vectors. Nucleic Acids Res. 23, 628–633 (1995).
    Article CAS PubMed PubMed Central Google Scholar
  12. Hacein-Bey-Abina, S. et al. Sustained correction of X-linked severe combined immunodeficiency by ex vivo gene therapy. N. Engl. J. Med. 346, 1185–1193 (2002).
    Article CAS PubMed Google Scholar
  13. Kiem, H.P. et al. Gene transfer into marrow repopulating cells: comparison between amphotropic and gibbon ape leukemia virus pseudotyped retroviral vectors in a competitive repopulation assay in baboons. Blood 90, 4638–4645 (1997).
    CAS PubMed Google Scholar
  14. Kelly, P.F., Vandergriff, J., Nathwani, A., Nienhuis, A.W. & Vanin, E.F. Highly efficient gene transfer into cord blood nonobese diabetic/severe combined immunodeficiency repopulating cells by oncoretroviral vector particles pseudotyped with the feline endogenous retrovirus (RD114) envelope protein. Blood 96, 1206–1214 (2000).
    CAS PubMed Google Scholar
  15. Cosset, F.-L., Takeuchi, Y., Battini, J.-L., Weiss, R.A. & Collins, M.K. High-titer packaging cells producing recombinant retroviruses resistant to human serum. J. Virol. 69, 7430–7436 (1995).
    CAS PubMed PubMed Central Google Scholar
  16. Marandin, A. et al. Retrovirus-mediated gene transfer into human CD34+38low primitive cells capable of reconstituting long-term cultures in vitro and nonobese diabetic-severe combined immunodeficiency mice in vivo. Hum. Gene Ther. 9, 1497–1511 (1998).
    Article CAS PubMed Google Scholar
  17. Demaison, C. et al. High-level transduction and gene expression in hematopoietic repopulation cells using a human immunodeficiency virus type 1–based lentiviral vector containing an internal spleen focus forming virus promoter. Hum. Gene Ther. 13, 803–813 (2002).
    Article CAS PubMed Google Scholar
  18. Schroder, A.R. et al. HIV-1 integration in the human genome favors active genes and local hotspots. Cell 110, 521–529 (2002).
    Article CAS PubMed Google Scholar
  19. Zufferey, R., Nagy, D., Mandel, R.J., Naldini, L. & Trono, D. Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Nat. Biotechnol. 15, 871–875 (1997).
    Article CAS PubMed Google Scholar
  20. Besnier, C., Takeuchi, Y. & Towers, G. Restriction of lentivirus in monkeys. Proc. Natl. Acad. Sci. USA 99, 11920–11925 (2002).
    Article CAS PubMed PubMed Central Google Scholar

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