Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana (original) (raw)

Nature Biotechnology volume 21, pages 1215–1221 (2003) Cite this article

Abstract

Understanding the functional connections between genes, proteins, metabolites and mineral ions is one of biology's greatest challenges in the postgenomic era. We describe here the use of mineral nutrient and trace element profiling as a tool to determine the biological significance of connections between a plant's genome and its elemental profile. Using inductively coupled plasma spectroscopy, we quantified 18 elements, including essential macro- and micronutrients and various nonessential elements, in shoots of 6,000 mutagenized M2 Arabidopsis thaliana plants. We isolated 51 mutants with altered elemental profiles. One mutant contains a deletion in FRD3, a gene known to control iron-deficiency responses in A. thaliana. Based on the frequency of elemental profile mutations, we estimate 2–4% of the A. thaliana genome is involved in regulating the plant's nutrient and trace element content. These results demonstrate the utility of elemental profiling as a useful functional genomics tool.

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Acknowledgements

This project is part of a larger collaborative effort funded by the National Science Foundation Plant Functional Genomics program (0077378-DBI) awarded to Mary Lou Guerinot, David Eide, Jeff Harper, David E. Salt and Julian Schroeder. More details about the collaborators and project can be found at http://plantst.sdsc.edu/. We also thank Venugopal Naga Venkata Gudimetla and Yanrong Zhaoy for assistance with database design and data analysis.

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Authors and Affiliations

  1. Center for Plant Environmental Stress Physiology, Horticulture Building, 625 Agriculture Mall Drive, Purdue University, West Lafayette, 47907, Indiana, USA
    Brett Lahner, Mehrzad Mahmoudian, Ellen L Smith & David E Salt
  2. Division of Biological Science, Cell and Developmental Biology Section and Center for Molecular Genetics 0116, University of California, San Diego, 9500 Gilman Drive, La Jolla, 92093, California, USA
    Jiming Gong, Khush B Abid & Julian I Schroeder
  3. Department of Nutritional Sciences, 217B Gwynn Hall, University of Missouri, Columbia, 65211, Missouri, USA
    Elizabeth E Rogers
  4. Department of Biological Sciences, 6044 Gilman, Dartmouth College, Hanover, 03755, New Hampshire, USA
    Mary L Guerinot
  5. Department of Cell Biology, The Scripps Research Institute, Mail Drop BCC283, 10550 North Torrey Pines Road, La Jolla, 92037, California, USA
    Jeffrey F Harper
  6. Department of Plant Biology, University of Minnesota, 250 Biological Science Center, 1445 Gortner Avenue, St. Paul, 55108, Minnesota, USA
    John M Ward
  7. Department of Agronomy, Agricultural Genomics, 915 State Street, Purdue University, West Lafayette, 47907, Indiana, USA
    Lauren McIntyre

Authors

  1. Brett Lahner
  2. Jiming Gong
  3. Mehrzad Mahmoudian
  4. Ellen L Smith
  5. Khush B Abid
  6. Elizabeth E Rogers
  7. Mary L Guerinot
  8. Jeffrey F Harper
  9. John M Ward
  10. Lauren McIntyre
  11. Julian I Schroeder
  12. David E Salt

Corresponding author

Correspondence toDavid E Salt.

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Competing interests

The authors declare no competing financial interests.

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Lahner, B., Gong, J., Mahmoudian, M. et al. Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana.Nat Biotechnol 21, 1215–1221 (2003). https://doi.org/10.1038/nbt865

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