Dynein binds to β-catenin and may tether microtubules at adherens junctions (original) (raw)

Nature Cell Biology volume 3, pages 913–917 (2001)Cite this article

Abstract

Interactions between microtubule and actin networks are thought to be crucial for mechanical and signalling events at the cell cortex. Cytoplasmic dynein has been proposed to mediate many of these interactions1,2,3. Here, we report that dynein is localized to the cortex at adherens junctions in cultured epithelial cells and that this localization is sensitive to drugs that disrupt the actin cytoskeleton. Dynein is recruited to developing contacts between cells, where it localizes with the junctional proteins β-catenin and E-cadherin. Microtubules project towards these early contacts and we hypothesize that dynein captures and tethers microtubules at these sites. Dynein immunoprecipitates with β-catenin, and biochemical analysis shows that dynein binds directly to β-catenin. Overexpression of β-catenin disrupts the cellular localization of dynein and also dramatically perturbs the organization of the cellular microtubule array. In cells overexpressing β-catenin, the centrosome becomes disorganized and microtubules no longer appear to be anchored at the cortex. These results identify a novel role for cytoplasmic dynein in capturing and tethering microtubules at adherens junctions, thus mediating cross-talk between actin and microtubule networks at the cell cortex.

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References

  1. Karki, S. & Holzbaur, E. L. F. Curr. Opin. Cell. Biol. 11, 45–53 (1999).
    Article CAS Google Scholar
  2. Goode, B. L., Drubin, D. G. & Barnes, G. Curr. Opin. Cell. Biol. 12, 63–71 (2000).
    Article CAS Google Scholar
  3. Fuchs, E. & Karakesisoglou, I. Genes Dev. 15, 1–14 (2001).
    Article CAS Google Scholar
  4. Steinberg, M. S. & McNutt, P. M. Curr. Opin. Cell. Biol. 11, 554–560 (1999).
    Article CAS Google Scholar
  5. Vasioukhin, V. & Fuchs, E. Curr. Opin. Cell. Biol. 13, 76–84 (2001).
    Article CAS Google Scholar
  6. Waterman-Storer, C. M., Salmon, W. C. & Salmon, E. D. Mol. Biol. Cell 11, 2471–2483 (2000).
    Article CAS Google Scholar
  7. King, S. M. et al. Biochemistry 37, 15033–15041 (1998).
    Article CAS Google Scholar
  8. Tai, A. W., Chuang, J. -Z. & Sung, C.-H. J. Cell Biol. 153, 1499–1509 (2001).
    Article CAS Google Scholar
  9. Vasioukhin, V., Bauer, C., Yin, M. & Fuchs, E. Cell 100, 209–219 (2000).
    Article CAS Google Scholar
  10. Karki, S., LaMonte, B. & Holzbaur E. L. F. J. Cell Biol. 142, 1023–1034 (1998).
    Article CAS Google Scholar
  11. Barth, A. I. M., Pollack, A. L., Altschuler, Y., Mostov, K. E. & Nelson, W. J. J. Cell Biol. 136, 693–706 (1997).
    Article CAS Google Scholar
  12. Tokito, M. K., Howland, D. S., Lee, V. M. -Y. & Holzbaur E. L. F. Mol. Biol. Cell 7, 1167–1180 (1996).
    Article CAS Google Scholar
  13. Karki, S., Tokito, M. K. & Holzbaur E. L. F. J. Biol. Chem. 272, 5887–5891 (1997).
    Article CAS Google Scholar
  14. Huber, A. H., Nelson, W. J. & Weis, W. I. Cell 90, 871–882 (1997).
    Article CAS Google Scholar

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Acknowledgements

This work was funded by a US NIH grant to E.L.F.H. and by postdoctoral fellowships from the US NIH to L.A.L. and the American Heart Association Southeastern Affiliate to S.K. E.L.F.H. is an Established Investigator of the American Heart Association.

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Authors and Affiliations

  1. Department of Animal Biology, University of Pennsylvania, Philadelphia, 19104, Pennsylvania, USA
    Lee A. Ligon, Sher Karki, Mariko Tokito & Erika L. F. Holzbaur

Authors

  1. Lee A. Ligon
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  2. Sher Karki
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  3. Mariko Tokito
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  4. Erika L. F. Holzbaur
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Corresponding author

Correspondence toErika L. F. Holzbaur.

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Ligon, L., Karki, S., Tokito, M. et al. Dynein binds to β-catenin and may tether microtubules at adherens junctions.Nat Cell Biol 3, 913–917 (2001). https://doi.org/10.1038/ncb1001-913

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