Reprogramming of the tumour microenvironment by stromal PTEN-regulated miR-320 (original) (raw)

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Acknowledgements

We thank Kara Batte for technical assistance with the microRNA platform, Rumeysa Biyik for bioinformatics assistance, Abdel-Rasoul Mahmoud for statistical assistance and the Ohio State University Human Tissue Resource Network and the Ohio State University Comprehensive Cancer Center Microarray, Nucleic Acids, Proteomic Shared Facilities for technical assistance. This work was funded by grants from the National Institutes of Health to M.C.O. (R01CA053271, P01CA097189) and to G.L. (R01CA85619, R01HD47470, P01CA097189) and from the Komen Breast Cancer Foundation and Evelyn Simmers Charitable Trust to M.C.O.

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Authors and Affiliations

1. Tumor Microenvironment Program, Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio 43210, USA
   A. Bronisz, G. Leone & M. C. Ostrowski
2. Department of Molecular and Cellular Biochemistry, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA
   A. Bronisz, J. A. Wallace, A.S. Merchant, H. Mathsyaraja, R. Srinivasan, C. K. Martin, F. Li & M. C. Ostrowski
3. Department of Neurological Surgery, Dardinger Laboratory for Neuro-oncology and Neurosciences, The Ohio State University Medical Center and James Comprehensive Cancer Center, Columbus, Ohio 43210, USA
   J. Godlewski, M.O. Nowicki, S. E. Lawler & E. A. Chiocca
4. Department of Molecular Genetics, College of Biological Sciences, Comprehensive Cancer Center, Ohio State University, Columbus, Ohio 43210, USA
   A. J. Trimboli, F. Li, T. Pécot & G. Leone
5. Department of Molecular Virology, Immunology and Medical Genetics, Ohio State University, Columbus, Ohio 43210, USA
   A. J. Trimboli, T. Pécot & G. Leone
6. Center for Biostatistics, Office of Health Sciences, The Ohio State University, Columbus, Ohio 43210, USA
   L. Yu & S. A. Fernandez
7. The Ohio State University Computer Science and Engineering, The Ohio State University Biomedical Informatics, The Ohio State University, Columbus, Ohio 43210, USA
   T. Pécot
8. Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210, USA
   T. J. Rosol
9. McGill Centre for Bioinformatics, McGill University, Montreal, Quebec, H3A0B1, Canada
   S. Cory & M. Hallett
10. Department of Biochemistry, Rosalind and Morris Goodman Cancer Center University, Montreal, Quebec, H3A0B, Canada
    S. Cory, M. Hallett & M. Park
11. Department of Oncology, McGill University, Quebec, H3A 1A1, Canada
    M. Park
12. Division of Pulmonary, Department of Internal Medicine, Allergy, Critical Care, and Sleep Medicine, Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA
    M. G. Piper & C. B. Marsh
13. Department of Surgery, School of Medicine, The Ohio State University, Columbus, Ohio 43210, USA
    L. D. Yee
14. Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905, USA
    R. E. Jimenez
15. Department of Pathology, The Ohio State University Medical Center and James Comprehensive Cancer Center, Columbus, Ohio 43210, USA
    G. Nuovo

Authors

1. A. Bronisz
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2. J. Godlewski
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3. J. A. Wallace
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4. A.S. Merchant
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5. M.O. Nowicki
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6. H. Mathsyaraja
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7. R. Srinivasan
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8. A. J. Trimboli
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9. C. K. Martin
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10. F. Li
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11. L. Yu
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12. S. A. Fernandez
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13. T. Pécot
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14. T. J. Rosol
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15. S. Cory
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16. M. Hallett
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17. M. Park
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18. M. G. Piper
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19. C. B. Marsh
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20. L. D. Yee
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21. R. E. Jimenez
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22. G. Nuovo
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23. S. E. Lawler
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24. E. A. Chiocca
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25. G. Leone
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26. M. C. Ostrowski
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Contributions

M.C.O. and G.L. designed and supervised this study, analysed data and helped write and edit the manuscript. A.B. and J.G. designed and carried out experiments, collected and analysed data and co-wrote the paper. J.A.W., H.M., R.S., A.J.T. and F.L. assisted technically with experiments, and collected and analysed data. M.O.N. assisted with fluorescent and confocal microscopy and immunohistochemistry. L.Y. and S.A.F. contributed to the statistical analyses of data and writing the manuscript. A.S.M., S.C., M.H., M.P. and T.P. contributed to the analysis and comparison of mouse and human profile data. M.G.P. and C.B.M. contributed to the analysis of microRNA data and writing the manuscript. C.K.M., L.D.Y., R.E.J., G.N. and T.J.R. contributed to the histopathological analysis of human samples and writing the manuscript. S.E.L. and E.A.C. contributed to the data analysis and writing the manuscript.

Corresponding authors

Correspondence toG. Leone or M. C. Ostrowski.

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The authors declare no competing financial interests.

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Bronisz, A., Godlewski, J., Wallace, J. et al. Reprogramming of the tumour microenvironment by stromal PTEN-regulated miR-320.Nat Cell Biol 14, 159–167 (2012). https://doi.org/10.1038/ncb2396

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• Received: 10 January 2011
• Accepted: 07 November 2011
• Published: 18 December 2011
• Issue Date: February 2012
• DOI: https://doi.org/10.1038/ncb2396