Dissecting the link between stress fibres and focal adhesions by CALI with EGFP fusion proteins (original) (raw)

References

  1. Wang, F.-S. & Jay, D. G. Chromophore-assisted laser inactivation (CALI): probing protein function in situ with a high degree of spatial and temporal resolution. Trends Cell Biol. 6, 442–445 (1996).
    Article CAS PubMed Google Scholar
  2. Jay, D. G. Selective destruction of protein function by chromophore-assisted laser inactivation. Proc. Natl Acad. Sci. USA 85, 5454–5458 (1988).
    Article CAS PubMed PubMed Central Google Scholar
  3. Liao, J. C., Roider, J. & Jay, D. G. Chromophore-assisted laser inactivation of proteins is mediated by the photogeneration of free radicals. Proc. Natl Acad. Sci. USA 91, 2659–2663 (1994).
    Article CAS PubMed PubMed Central Google Scholar
  4. Schmucker, D., Su, A. L., Beermann, A., Jackle, H. & Jay, D. Chromophore-assisted laser inactivation of patched protein switches cell fate in the larval visual system of Drosophila. Proc. Natl Acad. Sci. USA 91, 2664–2668 (1994).
    Article CAS PubMed PubMed Central Google Scholar
  5. Lamb, R. F. et al. Essential functions of ezrin in maintenance of cell shape and lamellipodial extension in normal and transformed fibroblasts. Curr. Biol. 7, 682–688 (1997).
    Article CAS PubMed Google Scholar
  6. Chang, H. Y. et al. Asymmetric retraction of growth cone filopodia following focal inactivation of calcineurin. Nature 376, 686–690 (1995).
    Article CAS PubMed Google Scholar
  7. Surrey, T. et al. Chromophore-assisted light inactivation and self-organization of microtubules and motors. Proc. Natl Acad. Sci. USA 95, 4293–4298 (1998).
    Article CAS PubMed PubMed Central Google Scholar
  8. Lazarides, E. & Burridge, K. Alpha-actinin: immunofluorescent localization of a muscle structural protein in nonmuscle cell. Cell 6, 289–298 (1975).
    Article CAS PubMed Google Scholar
  9. Gilmore, A. P. & Romer, L. H. Inhibition of focal adhesion kinase (FAK) signalling in focal adhesions decreases cell motility and proliferation. Mol. Biol. Cell 7, 1209–1224 (1996).
    Article CAS PubMed PubMed Central Google Scholar
  10. Otey, C. A. pp125FAK in the focal adhesion. Int. Rev. Cytol. 167, 161–183 (1996).
    Article CAS PubMed Google Scholar
  11. Edlund, M., Lotano, M. A. & Otey, C. A. Dynamics of α-actinin in focal adhesions and stress fibres visualized with α-actinin–green fluorescent protein. Cell Motil. Cytoskeleton 48, 175–189 (2001).
    Article Google Scholar
  12. Schaller, M. D. et al. pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions. Proc. Natl Acad. Sci. USA 89, 5192–5196 (1992).
    Article CAS PubMed PubMed Central Google Scholar
  13. Hanks, S. K., Calalb, M. B., Harper, M. C. & Patel, S. K. Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment to fibronectin. Proc. Natl Acad. Sci. USA 89, 8487–8491 (1992).
    Article CAS PubMed PubMed Central Google Scholar
  14. Burridge, K., Turner, C. E. & Romer, L. H. Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. J. Cell Biol. 119, 893–903 (1992).
    Article CAS PubMed Google Scholar
  15. Guan, J. L. & Shalloway, D. Regulation of focal adhesion-associated protein tyrosine kinase by both cellular adhesion and oncogenic transformation. Nature 358, 690–692 (1992).
    Article CAS PubMed Google Scholar
  16. Kornberg, L., Earp, H. S., Parsons, J. T., Schaller, M. & Juliano, R. L. Cell adhesion or integrin clustering increases phosphorylation of a focal adhesion-associated tyrosine kinase. J. Biol. Chem. 267, 23439–23442 (1992).
    CAS PubMed Google Scholar
  17. Romer, L. H., McLean, N., Turner, C. E. & Burridge, K. Tyrosine kinase activity, cytoskeletal organization, and motility in human vascular endothelial cells. Mol. Biol. Cell 5, 349–361 (1994).
    Article CAS PubMed PubMed Central Google Scholar
  18. Richardson, A. & Parsons, T. A mechanism for regulation of the adhesion-associated protein tyrosine kinase pp125FAK. Nature 380, 538–540 (1996).
    Article CAS PubMed Google Scholar
  19. Ilic, D. et al. Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice. Nature 377, 539–544 (1995).
    Article CAS PubMed Google Scholar
  20. Schaller, M. D. Biochemical signals and biological responses elicited by the focal adhesion kinase. Biochim. Biophys. Acta 1540, 1–21 (2001).
    Article CAS PubMed Google Scholar
  21. Cooley, M. A., Broome, J. M., Ohngemach, C., Romer, L. H. & Schaller, M. D. Paxillin binding is not sole determinant of focal adhesion localization or dominant-negative activity of focal adhesion kinase/focal adhesion kinase-related nonkinase. Mol. Biol. Cell 11, 3247–3263 (2000).
    Article CAS PubMed PubMed Central Google Scholar
  22. Pavalko, F. M., Otey, C. A., Simon, K. O. & Burridge, K. Alpha-actinin: a direct link between actin and integrins. Biochem. Soc. Trans. 19, 1065–1069 (1991).
    Article CAS PubMed Google Scholar
  23. Otey, C. A., Vasquez, G. B., Burridge, K. & Erickson, B. W. Mapping of the α-actinin binding site with the β1 integrin cytoplasmic domain. J. Biol. Chem. 286, 21193–21197 (1993).
    Google Scholar
  24. Sampath, R., Gallagher, P. J. & Pavalko, F. M. Cytoskeletal interactions with the leukocyte integrin β2 cytoplasmic tail. J. Biol. Chem. 273, 33588–33594 (1998).
    Article CAS PubMed Google Scholar
  25. Greenwood, J. A., Theibert, A. B., Prestwich, G. D. & Murphy-Ulrich, J. E. Restructuring of focal adhesion plaques by PI 3-kinase: Regulation by PtdIns(3,4,5)-P3 binding to α-actinin. J. Cell Biol. 150, 627–641 (2000).
    Article CAS PubMed PubMed Central Google Scholar
  26. Otey, C. A., Pavalko, F. M. & Burridge, K. An interaction between α-actinin and the β1 integrin subunit in vitro. J. Cell Biol. 111, 721–729 (1990).
    Article CAS PubMed Google Scholar
  27. Pavalko, F. M. & LaRoche, S. M. Activation of human neutrophils induces an interaction between the integrin β2-subunit (CD18) and the actin binding protein α-actinin. J. Immunol. 151, 3795–3807 (1993).
    CAS PubMed Google Scholar
  28. Pavalko, F. M. & Burridge, K. Disruption of the actin cytoskeleton after microinjection of proteolytic fragments of α-actinin. J. Cell Biol. 114, 481–491 (1991).
    Article CAS PubMed Google Scholar
  29. Burridge, K. & Wodnicka-Chrzanowska, M. Focal adhesions, contractility, and signalling. Annu. Rev. Cell Dev. Biol. 12, 463–519 (1996).
    Article CAS PubMed Google Scholar
  30. Koppel, D. Fluorescence redistribution after photobleaching. A new multipoint analysis of membrane translational dynamics. Biophys. J. 28, 281–292 (1979).
    Article CAS PubMed PubMed Central Google Scholar

Download references