Reaction-based small-molecule fluorescent probes for chemoselective bioimaging (original) (raw)
Czarnik, A. W. Chemical communication in water using fluorescent chemosensors. Acc. Chem. Res.27, 302–308 (1994). ArticleCAS Google Scholar
Kim, H. N., Lee, M. H., Kim, H. J., Kim, J. S. & Yoon, J. A new trend in rhodamine-based chemosensors: application of spirolactam ring-opening to sensing ions. Chem. Soc. Rev.37, 1465–1472 (2008). ArticleCASPubMed Google Scholar
Jun, M. E., Roy, B. & Ahn, K. H. 'Turn-on' fluorescent sensing with 'reactive' probes. Chem. Commun.47, 7583–7601 (2011). ArticleCAS Google Scholar
Du, J., Hu, M., Fan, J. & Peng, X. Fluorescent chemodosimeters using 'mild' chemical events for the detection of small anions and cations in biological and environmental media. Chem. Soc. Rev.41, 4511–4535 (2012). ArticleCASPubMed Google Scholar
Nagano, T. et al. Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins. Anal. Chem.70, 2446–2453 (1998). ArticlePubMed Google Scholar
Nagano, T., Takizawa, H. & Hirobe, M. Reactions of nitric oxide with amines in the presence of dioxygen. Tetrahedron Lett.36, 8239–8242 (1995). ArticleCAS Google Scholar
Kojima, H. et al. Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore. Anal. Chem.73, 1967–1973 (2001). ArticleCASPubMed Google Scholar
Nagano, T., Gabe, Y., Urano, Y., Kikuchi, K. & Kojima, H. Highly sensitive fluorescence probes for nitric oxide based on boron dipyrromethene chromophore-rational design of potentially useful bioimaging fluorescence probe. J. Am. Chem. Soc.126, 3357–3367 (2004). ArticleCASPubMed Google Scholar
Sasaki, E. et al. Highly sensitive near-infrared fluorescent probes for nitric oxide and their application to isolated organs. J. Am. Chem. Soc.127, 3684–3685 (2005). ArticleCASPubMed Google Scholar
Terai, T., Urano, Y., Izumi, S., Kojima, H. & Nagano, T. A practical strategy to create near-infrared luminescent probes: conversion from fluorescein-based sensors. Chem. Commun.48, 2840–2842 (2012). ArticleCAS Google Scholar
Yang, Y. J. et al. A highly selective low-background fluorescent imaging agent for nitric oxide. J. Am. Chem. Soc.132, 13114–13116 (2010). ArticleCASPubMed Google Scholar
Song, B., Wang, G. L., Tan, M. Q. & Yuan, J. L. A europium(III) complex as an efficient singlet oxygen luminescence probe. J. Am. Chem. Soc.128, 13442–13450 (2006). ArticleCASPubMed Google Scholar
Lippert, A. R., De Bittner, G. C. V. & Chang, C. J. Boronate oxidation as a bioorthogonal reaction approach for studying the chemistry of hydrogen peroxide in living systems. Acc. Chem. Res.44, 793–804 (2011). ArticleCASPubMedPubMed Central Google Scholar
Chang, M. C. Y., Pralle, A., Isacoff, E. Y. & Chang, C. J. A selective, cell-permeable optical probe for hydrogen peroxide in living cells. J. Am. Chem. Soc.126, 15392–15393 (2004). ArticleCASPubMedPubMed Central Google Scholar
Lo, L. C. & Chu, C. Y. Development of highly selective and sensitive probes for hydrogen peroxide. Chem. Commun. 2728–2729 (2003).
Miller, E. W., Tulyathan, O., Isacoff, E. Y. & Chang, C. J. Molecular imaging of hydrogen peroxide produced for cell signaling. Nature Chem. Biol.3, 349–349 (2007). ArticleCAS Google Scholar
Du, L. P., Li, M. Y., Zheng, S. L. & Wang, B. H. Rational design of a fluorescent hydrogen peroxide probe based on the umbelliferone fluorophore. Tetrahedron Lett.49, 3045–3048 (2008). ArticleCASPubMedPubMed Central Google Scholar
Dickinson, B. C., Huynh, C. & Chang, C. J. A palette of fluorescent probes with varying emission colors for imaging hydrogen peroxide signaling in living cells. J. Am. Chem. Soc.132, 5906–5915 (2010). ArticleCASPubMedPubMed Central Google Scholar
Karton-Lifshin, N. et al. A unique paradigm for a turn-on near-infrared cyanine-based probe: noninvasive intravital optical imaging of hydrogen peroxide. J. Am. Chem. Soc.133, 10960–10965 (2011). ArticleCASPubMed Google Scholar
Srikun, D., Miller, E. W., Dornaille, D. W. & Chang, C. J. An ICT-based approach to ratiometric fluorescence imaging of hydrogen peroxide produced in living cells. J. Am. Chem. Soc.130, 4596–4597 (2008). ArticleCASPubMed Google Scholar
Dickinson, B. C. & Chang, C. J. A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells. J. Am. Chem. Soc.130, 9638–9639 (2008). ArticleCASPubMedPubMed Central Google Scholar
Srikun, D., Albers, A. E., Nam, C. I., Iavaron, A. T. & Chang, C. J. Organelle-targetable fluorescent probes for imaging hydrogen peroxide in living cells via SNAP-tag protein labeling. J. Am. Chem. Soc.132, 4455–4465 (2010). ArticleCASPubMedPubMed Central Google Scholar
Miller, E. W., Dickinson, B. C. & Chang, C. J. Aquaporin-3 mediates hydrogen peroxide uptake to regulate downstream intracellular signaling. Proc. Natl Acad. Sci. USA107, 15681–15686 (2010). ArticlePubMed Google Scholar
Dickinson, B. C., Peltier, J., Stone, D., Schaffer, D. V. & Chang, C. J. Nox2 redox signaling maintains essential cell populations in the brain. Nature Chem. Biol.7, 106–112 (2011). ArticleCAS Google Scholar
Van de Bittner, G. C., Dubikovskaya, E. A., Bertozzi, C. R. & Chang, C. J. In vivo imaging of hydrogen peroxide production in a murine tumor model with a chemoselective bioluminescent reporter. Proc. Natl Acad. Sci. USA107, 21316–21321 (2010). ArticlePubMed Google Scholar
Lippert, A. R., Gschneidtner, T. & Chang, C. J. Lanthanide-based luminescent probes for selective time-gated detection of hydrogen peroxide in water and in living cells. Chem. Commun.46, 7510–7512 (2010). ArticleCAS Google Scholar
Du, L. P. Y., Ni, N. T. Y., Li, M. Y. & Wang, B. H. A fluorescent hydrogen peroxide probe based on a 'click' modified coumarin fluorophore. Tetrahedron Lett.51, 1152–1154 (2010). ArticleCASPubMedPubMed Central Google Scholar
Charkoudian, L. K., Pham, D. M. & Franz, K. J. A pro-chelator triggered by hydrogen peroxide inhibits iron-promoted hydroxyl radical formation. J. Am. Chem. Soc.128, 12424–12425 (2006). ArticleCASPubMed Google Scholar
Wei, Y. & Guo, M. Hydrogen peroxide triggered prochelator activation, subsequent metal chelation, and attenuation of the fenton reaction. Angew. Chem. Int. Ed.46, 4722–4725 (2007). Article Google Scholar
Jourden, J. L. M. & Cohen, S. M. Hydrogen peroxide activated matrix metalloproteinase inhibitors: a prodrug approach. Angew. Chem. Int. Ed.49, 6795–6797 (2010). ArticleCAS Google Scholar
Kuang, Y. Y., Baakrishnan, K., Gandhi, V. & Peng, X. H. Hydrogen peroxide inducible DNA cross-linking agents: targeted anticancer prodrugs. J. Am. Chem. Soc.133, 19278–19281 (2011). ArticleCASPubMedPubMed Central Google Scholar
Sella, E. & Shabat, D. Self-immolative dendritic probe for direct detection of triacetone triperoxide. Chem. Commun. 5701–5703 (2008).
Broaders, K. E., Grandhe, S. & Frechet, J. M. J. A biocompatible oxidation-triggered carrier polymer with potential in therapeutics. J. Am. Chem. Soc.133, 756–758 (2011). ArticleCASPubMed Google Scholar
Cocheme, H. M. et al. Measurement of H2O2 within living Drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix. Cell Metab.13, 340–350 (2011). ArticleCASPubMedPubMed Central Google Scholar
Abo, M. et al. Development of a highly sensitive fluorescence probe for hydrogen peroxide. J. Am. Chem. Soc.133, 10629–10637 (2011). ArticleCASPubMed Google Scholar
Lippert, A. R., Keshari, K. R., Kurhanewicz, J. & Chang, C. J. A hydrogen peroxide-responsive hyperpolarized 13C MRI contrast agent. J. Am. Chem. Soc.133, 3776–3779 (2011). ArticleCASPubMedPubMed Central Google Scholar
Yang, D., Wang, H. L., Sun, Z. N., Chung, N. W. & Shen, J. G. A highly selective fluorescent probe for the detection and imaging of peroxynitrite in living cells. J. Am. Chem. Soc.128, 6004–6005 (2006). ArticleCASPubMed Google Scholar
Sun, Z. N. et al. BODIPY-based fluorescent probe for peroxynitrite detection and imaging in living cells. Org. Lett.11, 1887–1890 (2009). ArticleCASPubMed Google Scholar
Peng, T. & Yang, D. HKGreen-3: a rhodol-based fluorescent probe for peroxynitrite. Org. Lett.12, 4932–4935 (2010). ArticleCASPubMed Google Scholar
Zhang, W. J., Guo, C., Liu, L. H., Qin, J. G. & Yang, C. L. Naked-eye visible and fluorometric dual-signaling chemodosimeter for hypochlorous acid based on water-soluble _p_-methoxyphenol derivative. Org. Biomol. Chem.9, 5560–5563 (2011). ArticleCASPubMed Google Scholar
Koide, Y., Urano, Y., Hanaoka, K., Terai, T. & Nagano, T. Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging. J. Am. Chem. Soc.133, 5680–5682 (2011). ArticleCASPubMed Google Scholar
Setsukinai, K., Urano, Y., Kakinuma, K., Majima, H. J. & Nagano, T. Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species. J. Biol. Chem.278, 3170–3175 (2003). ArticlePubMed Google Scholar
Shepherd, J. et al. A fluorescent probe for the detection of myeloperoxidase activity in atherosclerosis-associated macrophages. Chem. Biol.14, 1221–1231 (2007). ArticleCASPubMedPubMed Central Google Scholar
Yu, F. B. A. et al. A near-IR reversible fluorescent probe modulated by selenium for monitoring peroxynitrite and imaging in living cells. J. Am. Chem. Soc.133, 11030–11033 (2011). ArticleCASPubMed Google Scholar
Garner, A. L. et al. Specific fluorogenic probes for ozone in biological and atmospheric samples. Nature Chem.1, 316–321 (2009). ArticleCAS Google Scholar
Lippert, A. R., New, E. J. & Chang, C. J. Reaction-based fluorescent probes for selective imaging of hydrogen sulfide in living cells. J. Am. Chem. Soc.133, 10078–10080 (2011). ArticleCASPubMed Google Scholar
Peng, H. J. et al. A fluorescent probe for fast and quantitative detection of hydrogen sulfide in blood. Angew. Chem. Int. Ed.50, 9672–9675 (2011). ArticleCAS Google Scholar
Yu, F. B. A. et al. An ICT-based strategy to a colorimetric and ratiometric fluorescence probe for hydrogen sulfide in living cells. Chem. Commun.48, 2852–2854 (2012). ArticleCAS Google Scholar
Montoya, L. A. & Pluth, M. D. Selective turn-on fluorescent probes for imaging hydrogen sulfide in living cells. Chem. Commun.48, 4767–4769 (2012). ArticleCAS Google Scholar
Chen, S., Chen, Z. J., Ren, W. & Ai, H. W. Reaction-based genetically encoded fluorescent hydrogen sulfide sensors. J. Am. Chem. Soc.134, 9589–9592 (2012). ArticleCASPubMed Google Scholar
Qian, Y. et al. Selective fluorescent probes for live-cell monitoring of sulphide. Nature Commun.2, 495 (2011). ArticleCAS Google Scholar
Liu, C. R. et al. Capture and visualization of hydrogen sulfide by a fluorescent probe. Angew. Chem. Int. Ed.50, 10327–10329 (2011). ArticleCAS Google Scholar
Maeda, H. et al. 2,4-Dinitrobenzenesulfonyl fluoresceins as fluorescent alternatives to Ellman's reagent in thiol-quantification enzyme assays. Angew. Chem. Int. Ed.44, 2922–2925 (2005). ArticleCAS Google Scholar
Maeda, H. et al. A design of fluorescent probes for superoxide based on a nonredox mechanism. J. Am. Chem. Soc.127, 68–69 (2005). ArticleCASPubMed Google Scholar
Jiang, W., Fu, Q. Q., Fan, H. Y., Ho, J. & Wang, W. A highly selective fluorescent probe for thiophenols. Angew. Chem. Int. Ed.46, 8445–8448 (2007). ArticleCAS Google Scholar
Bouffard, J., Kim, Y., Swager, T. M., Weissleder, R. & Hilderbrand, S. A. A highly selective fluorescent probe for thiol bioimaging. Org. Lett.10, 37–40 (2008). ArticleCASPubMed Google Scholar
Pires, M. M. & Chmielewski, J. Fluorescence imaging of cellular glutathione using a latent rhodamine. Org. Lett.10, 837–840 (2008). ArticleCASPubMed Google Scholar
Nguyen, B. T. & Anslyn, E. V. Indicator-displacement assays. Coord. Chem. Rev.250, 3118–3127 (2006). ArticleCAS Google Scholar
Fabbrizzi, L., Licchelli, M., Pallavicini, P., Sacchi, D. & Taglietti, A. Sensing of transition metals through fluorescence quenching or enhancement—a review. Analyst121, 1763–1768 (1996). ArticleCAS Google Scholar
Lim, M. H. & Lippard, S. J. Fluorescence-based nitric oxide detection by ruthenium porphyrin fluorophore complexes. Inorg. Chem.43, 6366–6370 (2004). ArticleCASPubMed Google Scholar
Katayama, Y., Takahashi, S. & Maeda, M. Design, synthesis and characterization of a novel fluorescent probe for nitric oxide (nitrogen monoxide). Anal. Chim. Acta365, 159–167 (1998). ArticleCAS Google Scholar
Franz, K. J., Singh, N. & Lippard, S. J. Metal-based NO sensing by selective ligand dissociation. Angew. Chem. Int. Ed.39, 2120–2122 (2000). ArticleCAS Google Scholar
Hilderbrand, S. A., Lim, M. H. & Lippard, S. J. Dirhodium tetracarboxylate scaffolds as reversible fluorescence-based nitric oxide sensors. J. Am. Chem. Soc.126, 4972–4978 (2004). ArticleCASPubMed Google Scholar
Royzen, M., Dai, Z. H. & Canary, J. W. Ratiometric displacement approach to Cu(II) sensing by fluorescence. J. Am. Chem. Soc.127, 1612–1613 (2005). ArticleCASPubMed Google Scholar
Wu, Q. Y. & Anslyn, E. V. Catalytic signal amplification using a Heck reaction. an example in the fluorescence sensing of Cu(II). J. Am. Chem. Soc.126, 14682–14683 (2004). ArticleCASPubMed Google Scholar
Ojida, A. et al. Bis(Dpa-Zn-II) appended xanthone: excitation ratiometric chemosensor for phosphate anions. Angew. Chem. Int. Ed.45, 5518–5521 (2006). ArticleCAS Google Scholar
Ojida, A. et al. Design of dual-emission chemosensors for ratiometric detection of ATP derivatives. Chem. Asian J.1, 555–563 (2006). ArticleCASPubMed Google Scholar
Choi, M. G., Cha, S., Lee, H., Jeon, H. L. & Chang, S. K. Sulfide-selective chemosignaling by a Cu2 complex of dipicolylamine appended fluorescein. Chem. Commun. 7390–7392 (2009).
Sasakura, K. et al. Development of a highly selective fluorescence probe for hydrogen sulfide. J. Am. Chem. Soc.133, 18003–18005 (2011). ArticleCASPubMed Google Scholar
Tsuge, K., DeRosa, F., Lim, M. D. & Ford, P. C. Intramolecular reductive nitrosylation: reaction of nitric oxide and a copper(II) complex of a cyclam derivative with pendant luminescent chromophores. J. Am. Chem. Soc.126, 6564–6565 (2004). ArticleCASPubMed Google Scholar
Lim, M. H., Xu, D. & Lippard, S. J. Visualization of nitric oxide in living cells by a copper-based fluorescent probe. Nature Chem. Biol.2, 375–380 (2006). ArticleCAS Google Scholar
McQuade, L. E. et al. Visualization of nitric oxide production in the mouse main olfactory bulb by a cell-trappable copper(II) fluorescent probe. Proc. Natl Acad. Sci. USA107, 8525–8530 (2010). ArticlePubMed Google Scholar
Pluth, M. D., Chan, M. R., McQuade, L. E. & Lippard, S. J. Seminaphthofluorescein-based fluorescent probes for imaging nitric oxide in live cells. Inorg. Chem.50, 9385–9392 (2011). ArticleCASPubMedPubMed Central Google Scholar
Hitomi, Y., Takeyasu, T., Funabiki, T. & Kodera, M. Detection of enzymatically generated hydrogen peroxide by metal-based fluorescent probe. Anal. Chem.83, 9213–9216 (2011). ArticleCASPubMed Google Scholar
Song, D. et al. A fluorescence turn-on H2O2 probe exhibits lysosome-localized fluorescence signals. Chem. Commun.48, 5449–5451 (2012). ArticleCAS Google Scholar
Domaille, D. W., Que, E. L. & Chang, C. J. Synthetic fluorescent sensors for studying the cell biology of metals. Nature Chem. Biol.4, 168–175 (2008). ArticleCAS Google Scholar
Que, E. L., Domaille, D. W. & Chang, C. J. Metals in neurobiology: probing their chemistry and biology with molecular imaging. Chem. Rev.108, 1517–1549 (2008). ArticleCASPubMed Google Scholar
Chae, M. Y. & Czarnik, A. W. Fluorometric chemodosimetry. Mercury(II) and silver(I) indication in water via enhanced fluorescence signaling. J. Am. Chem. Soc.114, 9704–9705 (1992). ArticleCAS Google Scholar
Dujols, V., Ford, F. & Czarnik, A. W. A long-wavelength fluorescent chemodosimeter selective for Cu(II) ion in water. J. Am. Chem. Soc.119, 7386–7387 (1997). ArticleCAS Google Scholar
Guo, Z., Zhu, W. H., Zhu, M. M., Wu, X. M. & Tian, H. Near-infrared cell-permeable Hg2-selective ratiometric fluorescent chemodosimeters and fast indicator paper for MeHg+ based on tricarbocyanines. Chem. Eur. J.16, 14424–14432 (2010). ArticleCASPubMed Google Scholar
Ko, S. K., Yang, Y. K., Tae, J. & Shin, I. In vivo monitoring of mercury ions using a rhodamine-based molecular probe. J. Am. Chem. Soc.128, 14150–14155 (2006). ArticleCASPubMed Google Scholar
Zhang, X. L., Xiao, Y. & Qian, X. H. A ratiometric fluorescent probe based on FRET for imaging Hg2 ions in living cells. Angew. Chem. Int. Ed.47, 8025–8029 (2008). ArticleCAS Google Scholar
Lee, M. H., Lee, S. W., Kim, S. H., Kang, C. & Kim, J. S. Nanomolar Hg(II) detection using Nile blue chemodosimeter in biological media. Org. Lett.11, 2101–2104 (2009). ArticleCASPubMed Google Scholar
Shi, W. & Ma, H. Rhodamine B thiolactone: a simple chemosensor for Hg2 in aqueous media. Chem. Commun. 1856–1858 (2008).
Zhan, X.-Q., Qian, Z.-H., Zheng, H., Su, B.-Y., Lan, Z. & Xu, J.-G. Rhodamine thiospirolactone. highly selective and sensitive reversible sensing of Hg(II). Chem. Commun. 1859–1861 (2008).
Kim, J. H. et al. Fluorescent coumarinyldithiane as a selective chemodosimeter for mercury(II) ion in aqueous solution. Tetrahedron Lett.50, 5958–5961 (2009). ArticleCAS Google Scholar
Rao, A. S. et al. Reaction-based two-photon probes for mercury ions: fluorescence imaging with dual optical windows. Org. Lett.14, 2598–2601 (2012). ArticleCASPubMed Google Scholar
Kierat, R. M. & Kramer, R. A fluorogenic and chromogenic probe that detects the esterase activity of trace copper(II). Bioorg. Med. Chem. Lett.15, 4824–4827 (2005). ArticleCASPubMed Google Scholar
Kovacs, J. & Mokhir, A. Catalytic hydrolysis of esters of 2-hydroxypyridine derivatives for Cu2 detection. Inorg. Chem.47, 1880–1882 (2008). ArticleCASPubMed Google Scholar
Chatterjee, A. et al. Selective fluorogenic and chromogenic probe for detection of silver ions and silver nanoparticles in aqueous media. J. Am. Chem. Soc.131, 2040–2041 (2009). ArticleCASPubMed Google Scholar
Zhou, Z. & Fahrni, C. J. A fluorogenic probe for the copper(I)-catalyzed azide-alkyne ligation reaction: modulation of the fluorescence emission via 3(n,π*)-1 (π,π*) inversion. J. Am. Chem. Soc.126, 8862–8863 (2004). ArticleCASPubMed Google Scholar
Le Droumaguet, C., Wang, C. & Wang, Q. Fluorogenic click reaction. Chem. Soc. Rev.39, 1233–1239 (2010). ArticleCASPubMed Google Scholar
Viguier, R. F. H. & Hulme, A. N. A sensitized europium complex generated by micromolar concentrations of copper(I): toward the detection of copper(I) in biology. J. Am. Chem. Soc.128, 11370–11371 (2006). ArticleCASPubMed Google Scholar
Garner, A. L. & Koide, K. Studies of a fluorogenic probe for palladium and platinum leading to a palladium-specific detection method. Chem. Commun. 86–88 (2009).
Garner, A. L. & Koide, K. Oxidation state-specific fluorescent method for palladium(II) and platinum(IV) based on the catalyzed aromatic Claisen rearrangement. J. Am. Chem. Soc.130, 16472–16473 (2008). ArticleCASPubMed Google Scholar
Santra, M., Ko, S. K., Shin, I. & Ahn, K. H. Fluorescent detection of palladium species with an _O_-propargylated fluorescein. Chem. Commun.46, 3964–3966 (2010). ArticleCAS Google Scholar
Zhu, B. C. et al. A 4-hydroxynaphthalimide-derived ratiometric fluorescent chemodosimeter for imaging palladium in living cells. Chem. Commun.47, 8656–8658 (2011). ArticleCAS Google Scholar
Song, F. L., Watanabe, S., Floreancig, P. E. & Koide, K. Oxidation-resistant fluorogenic probe for mercury based on alkyne oxymercuration. J. Am. Chem. Soc.130, 16460–16461 (2008). ArticleCASPubMed Google Scholar
Ando, S. & Koide, K. Development and applications of fluorogenic probes for mercury(II) based on vinyl ether oxymercuration. J. Am. Chem. Soc.133, 2556–2566 (2011). ArticleCASPubMedPubMed Central Google Scholar
Santra, M. et al. A chemodosimeter approach to fluorescent sensing and imaging of inorganic and methylmercury species. Chem. Commun. 2115–2117 (2009).
Lin, W. Y., Cao, X. W., Ding, Y. D., Yuan, L. & Long, L. L. A highly selective and sensitive fluorescent probe for Hg2 imaging in live cells based on a rhodamine-thioamide–alkyne scaffold. Chem. Commun.46, 3529–3531 (2010). ArticleCAS Google Scholar
Taki, M., Iyoshi, S., Ojida, A., Hamachi, I. & Yamamoto, Y. Development of highly sensitive fluorescent probes for detection of intracellular copper(I) in living systems. J. Am. Chem. Soc.132, 5938–5939 (2010). ArticleCASPubMed Google Scholar
Au-Yeung, H. Y., New, E. J. & Chang, C. J. A selective reaction-based fluorescent probe for detecting cobalt in living cells. Chem. Commun.48, 5268–5270 (2012). ArticleCAS Google Scholar
De Silva, A. P. et al. Signaling recognition events with fluorescent sensors and switches. Chem. Rev.97, 1515–1566 (1997). ArticleCASPubMed Google Scholar
Palmer, A. E. & Tsien, R. Y. Measuring calcium signaling using genetically targetable fluorescent indicators. Nature Protoc.1, 1057–1065 (2006). ArticleCAS Google Scholar